Growth of Escherichia coli Under Extremely Low-Frequency Electromagnetic Fields

Justo, Oselys Rodríguez; Pérez, Víctor Haber; Alvarez, David Chacón; Alegre, Ranulfo Monte

doi:10.1385/abab:134:2:155

Cited by 66 publications

(39 citation statements)

References 10 publications

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“…11 Therefore, in this work an extremely low-frequency electromagnetic field (ELF) was applied to an E. coli cell suspension during the fed-batch fermentation process to verify its influence on cell proliferation and recombinant gp41 protein production. The culture medium was recycled continuously through an external recycle loop coupled with a spiral U-shaped tube disposed inside the magnetic field generator.…”

Section: Introductionmentioning

confidence: 99%

gp‐41 protein production by E. coli in unconventional bioreactor coupled with magnetic field generator

Justo¹,

Pérez²,

Alvarez³

2007

J of Chemical Tech & Biotech

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BACKGROUND: In this work recombinant gp-41 protein production by E. coli in an unconventional bioreactor coupled with a magnetic field generator was studied. The submerged fermentation process was carried out by fed-batch operation, recycling the culture medium externally through a stainless steel spiral U-shape tube inserted on the inside of a magnetic field generator. The exposure time and magnetic field induction were varied in a range from 1 to 12 h and 10 to 100 mT, respectively, according to a central composite design with centered face.

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Section: Introductionmentioning

confidence: 99%

gp‐41 protein production by E. coli in unconventional bioreactor coupled with magnetic field generator

Justo¹,

Pérez²,

Alvarez³

2007

J of Chemical Tech & Biotech

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“…In terms of electromagnetic field action in bacteria, several papers have reported alterations on growth, DNA molecules and gene expression Potenza et al 2004b;El May et al 2009). Escherichia coli cells exposed to an extremely low frequency magnetic field (0.1 T) for 6.5 h exhibited changes in viability compared to unexposed cells, which had a viability 100 times higher than the control (Justo et al 2006). El May et al (2009 reported that a static magnetic field (200 mT) induced a decrease of colony-forming units (CFU) of Salmonella enterica subsp.…”

Section: Introductionmentioning

confidence: 91%

Static magnetic field increases the sensitivity of Salmonella to gentamicin

et al. 2010

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The present study was carried out to evaluate the effects of static magnetic field (SMF) on antibiotic sensitivity of Salmonella enterica subsp. enterica serovar Hadar. We have evaluated antibiotic susceptibility using the disc diffusion method following exposure to SMF. Our results showed that exposure to a 200-mT SMF static magnetic field increased the efficiency (p<0.01) of gentamicin against Salmonella Hadar but did not affect the diameter of the inhibition zone of some other antibiotics actives on Enterobacteria: penicillin, oxacillin, cephalotin, neomycin, amikacin, tetracyclin, erythromycin, spiramycin, chloramphenicol, nalidixic acid and vancomycin.

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“…A wide variety of responses involving magnetic field predominance have been summarized in Table 1. [6] AC MF 16, 60 Hz Enolase activity stimulation; Suppression of enolase activity [7] 0.05-1 mT Reduced transposition activity & enhanced cell viability [6] OMF 100 mT Exposure time dependent stimulation or inhibition of cell viability [8] 30 µT Cell density dependent changes in AVTD [9] DC EF NA Increase in growth, removal of inhibitory compounds in medium [10] AC MF 0. Growth increase and interestingly a loss of intercellular cohesion [16] Paper to be seen AC MF 0-0.3 Hz @ Elevated or even diminished growth rates for Bacillus subtilis, Candida albicans, Halobacterium, [17] 5−90 mT Salmonella typhimurium, and Staphylococci…”

Section: Group I: Treatments Involving Magnetic Field Predominancementioning

confidence: 99%

Electromagnetic Biostimulation of Living Cultures for Biotechnology, Biofuel and Bioenergy Applications

Hunt¹,

Zavalin²,

Bhatnagar³

et al. 2013

Advances in Biofuel Production

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Abstract:The surge of interest in bioenergy has been marked with increasing efforts in research and development to identify new sources of biomass and to incorporate cuttingedge biotechnology to improve efficiency and increase yields. It is evident that various microorganisms will play an integral role in the development of this newly emerging industry, such as yeast for ethanol and Escherichia coli for fine chemical fermentation.However, it appears that microalgae have become the most promising prospect for biomass production due to their ability to grow fast, produce large quantities of lipids, carbohydrates and proteins, thrive in poor quality waters, sequester and recycle carbon dioxide from industrial flue gases and remove pollutants from industrial, agricultural and municipal wastewaters. In an attempt to better understand and manipulate microorganisms for optimum production capacity, many researchers have investigated alternative methods for stimulating their growth and metabolic behavior. One such novel approach is the use of electromagnetic fields for the stimulation of growth and metabolic cascades and controlling biochemical pathways. An effort has been made in this review to consolidate the information on the current status of biostimulation research to enhance microbial growth and metabolism using electromagnetic fields. It summarizes information on the biostimulatory effects on growth and other biological processes to obtain insight regarding OPEN ACCESS Int. J. Mol. Sci. 2009, 104516 factors and dosages that lead to the stimulation and also what kind of processes have been reportedly affected. Diverse mechanistic theories and explanations for biological effects of electromagnetic fields on intra and extracellular environment have been discussed. The foundations of biophysical interactions such as bioelectromagnetic and biophotonic communication and organization within living systems are expounded with special consideration for spatiotemporal aspects of electromagnetic topology, leading to the potential of multipolar electromagnetic systems. The future direction for the use of biostimulation using bioelectromagnetic, biophotonic and electrochemical methods have been proposed for biotechnology industries in general with emphasis on an holistic biofuel system encompassing production of algal biomass, its processing and conversion to biofuel.

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Growth of Escherichia coli Under Extremely Low-Frequency Electromagnetic Fields

Cited by 66 publications

References 10 publications

gp‐41 protein production by E. coli in unconventional bioreactor coupled with magnetic field generator

gp‐41 protein production by E. coli in unconventional bioreactor coupled with magnetic field generator

Static magnetic field increases the sensitivity of Salmonella to gentamicin

Electromagnetic Biostimulation of Living Cultures for Biotechnology, Biofuel and Bioenergy Applications

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