Growth of Candida albicans in Saliva: Stimulation by Glucose Associated with Antibiotics, Corticosteroids, and Diabetes Mellitus

Knight, L S; Fletcher, J.

doi:10.1093/infdis/123.4.371

Cited by 221 publications

(124 citation statements)

References 14 publications

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“…In clinical terms, an elevated intra-oral concentration of dietary sugars could suppress the formation of phospholipase, thereby reducing the pathogenic potential of this yeast. While this effect may be beneficial to the host, it could on the other hand lead to increased proliferation of oral yeast (15) and augmented adhesion of yeast to the epithelium (31).…”

Section: Discussionmentioning

confidence: 99%

Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children

Mardegan¹,

Klein²,

Golvea³

et al. 2006

Braz. J. Microbiol.

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Candida albicans oral strains collected from caries-free and caries-active healthy children ranging from 24 to 36 months old, were studied. The aim of the study was to determine proteinase and phospholipase activities produced by Candida albicans in the two groups and to determine the phenotypic diversity of these enzymes based on genetic polymorphism using the AP-PCR method. Strains identified by morphological and fermentation tests as C. albicans were grown in proteinase and phospholipase agar media at 37ºC for 7 and 4 days, respectively. After the incubation period, the enzyme activity of the proteinase and phospholipase positive strains was measured. All strains were subjected to AP-PCR, using the arbitrary primer AP-3. The enzymatic analysis showed no differences between the two groups. The AP-PCR method was effective in demonstrating intra-individual genetic polymorphism in C. albicans, showing a greater clonal diversity in caries-active versus caries-free children. Dendograms of similarity showed only intra-individual clonal lineage. The results suggest that the enzymatic profile does not depend on the genotypic characteristics of the strains.

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Section: Discussionmentioning

confidence: 99%

Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children

Mardegan¹,

Klein²,

Golvea³

et al. 2006

Braz. J. Microbiol.

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“…Not only would such an environment stimulate candidal proteases but high dietary carbohydrate could enhance the proliferation (Knight and Fletcher, 1971) and adhesion of C. albicans to palatal epithelial cells (Douglas, Houston and McCourtie, 198 1;Samaranayake and MacFarlane, 198 1, 1982) and acrylic denture surfaces (Samaranayake, McCourtie and MacFarlane, 1980;McCourtie and Douglas, 198 1).…”

Section: Discussionmentioning

confidence: 99%

The proteolytic potential of Candida albicans in human saliva supplemented with glucose

Samaranayake

Hughes

MacFarlane

1984

Journal of Medical Microbiology

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“…Transformation from the yeast phase to the mycelial phase is also correlated with increased adhesion (7,10). Elevated concentrations of glucose in saliva or vaginal fluids dramatically increase the ability of candida to adhere or to replicate actively at local tissue sites (12,13); indeed, in one study, increased concentrations of salivary glucose in diabetic patients were the single most important influence upon candidal replication ( 12).…”

Section: Introductionmentioning

confidence: 99%

Molecular mimicry in Candida albicans. Role of an integrin analogue in adhesion of the yeast to human endothelium.

Gustafson¹,

Vercellotti²,

Bendel³

et al. 1991

J. Clin. Invest.

110

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Hematogenous infection with the yeast Candida albicans now occurs with increasing frequency in the neonate, the immunocompromised patient, and the hyperglycemic or hyperalimented host. Yeast-phase C. albicans expresses a protein that is antigenically and structurally related to CD11b/CD18, a member of the 2 integrins and a well-characterized adhesin for mammalian neutrophils. Both the neutrophil protein and its analogue in C. albicans have an identical affinity for the C3 ligand iC3b, and both proteins are significantly increased in expression at 370C. Given these several similarities, we therefore studied the role of the integrin analogue on C. albicans in the adhesion of the yeast to human umbilical vein endothelium (HUVE).After growth of C. albicans in 20 mM D-glucose, as opposed to 20 mM L-glutamate, flow cytometric analysis with monoclonal antibodies recognizing the a-subunit of CDMMb/ CD18 demonstrated a 25.0% increase in mean channel fluorescence (range 18.4-31.8%), as well as an increased percentage of yeasts fluorescing (P < 0.02). This increased intensity of fluorescence, which corresponds to increased expression of the integrin analogue, also correlated with a significant increase of 30-80% in adhesion of glucose-grown C. albicans to HUVE (P < 0.02). Blockade of the integrin analogue on C. albicans by monoclonal antibodies recognizing adhesive epitopes on neutrophil CD11b/CD18 inhibited glucose-enhanced adhesion of C. albicans to HUVE. Incubation of glucose-grown C. albicans with saturating concentrations of purified human iC3b, the ligand for CD1lb/CD18, reduced adhesion of the yeast to HUVE by 49.7%, whereas BSA in equimolar concentration had no effect (P < 0.001). These results identify a glucose-responsive integrin analogue on C. albicans as one of possibly several cellular structures that mediate adhesion of the yeast to human endothelium. (J. Clin. Invest. 1991. 87:1896-1902

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Growth of Candida albicans in Saliva: Stimulation by Glucose Associated with Antibiotics, Corticosteroids, and Diabetes Mellitus

Cited by 221 publications

References 14 publications

Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children

Biotyping and genotypic diversity among oral Candida albicans strains from caries-free and caries-active healthy children

The proteolytic potential of Candida albicans in human saliva supplemented with glucose

Molecular mimicry in Candida albicans. Role of an integrin analogue in adhesion of the yeast to human endothelium.

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