Growth of Avena coleoptiles and pH drop of protoplast suspensions induced by chlorinated indoleacetic acids

Böttger, Michael; Engvild, Kjeld C.; Soll, Hans-Jürgen

doi:10.1007/bf00389385

Cited by 67 publications

(32 citation statements)

References 15 publications

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“…Chlorinated auxin, 4-Cl-IAA, occurs naturally in a number of plants, mainly members of the Fabaceae [18][19][20][21][22][23], but it has not been found in maize [22]. 4-Cl-IAA has been tested in many different bioassays that have shown its exceptionally high level of biological activity as compared to IAA [24][25][26][27][28][29][30][31][32]. The aim of this investigation was to compare the effect of two auxins (IAA and 4-Cl-IAA), at a wide range of concentrations (10 -12 -10 -6 M), on the redox activity and proton extrusion in maize root segments.…”

Section: Introductionmentioning

confidence: 99%

The effect of auxins (IAA and 4-Cl-IAA) on the redox activity and medium pH of Zea mays L. root segments

Lekacz

Karcz

2006

Cellular and Molecular Biology Letters

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Indole-3-acetic acid (IAA) and 4-chloroindole-3-acetic acid (4-Cl-IAA) were tested at different concentrations and times for their capacity to change the redox activity and medium pH of maize root segments. The doseresponse surfaces (dose-response curves as a function of time) plotted for redox activity and changes in medium pH (expressed as ΔpH) had a similar shape for both auxins, but differed significantly at the optimal concentrations. With 4-Cl-IAA, the maximal values of redox activity and medium pH changes were observed at 10 -10 M, which was a 100-fold lower concentration than with IAA. Correlations were observed between redox activity and medium pH changes at the optimal concentrations of both IAA and 4-Cl-IAA. The results are discussed herein, taking into account both the concentration of the auxins and the effects produced by them.

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Section: Introductionmentioning

confidence: 99%

The effect of auxins (IAA and 4-Cl-IAA) on the redox activity and medium pH of Zea mays L. root segments

Lekacz

Karcz

2006

Cellular and Molecular Biology Letters

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“…30 The compound recently isolated from Pistacia integrrima may be tested for its biological activities as cholrinated compounds isolated from Pea plant are known to most active biological compounds. 31 Author is towards more experimentation to overall finalize the structure of this novel compound from Pistacia integerrima.…”

Section: Resultsmentioning

confidence: 99%

Isolation Studies From Stem Extract of Pistacia Integerrima Stew. Ex Brand

Bibi

Qayyum

Nisa

et al. 2016

J. Chil. Chem. Soc.

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Plants have been source of fantastic natural compounds that range in complexity. Chemical studies on traditional medicinal plants have led to discovery of potent bioactive components. Pistacia integerrima belonging to family Anacardiaceae has been traditionally effective for rheumatic pain, analgesic and antipyretic effects and used in treatment of coughs, phthisis, asthma and dysentery. Natural products have served as basis for compounds to be utilized in drug discovery. Stem extract of Pistacia integerrima was investigated with the aim to find some novel components that can later be used as drugs or active pharmaceutical intermediates. Plant material was collected from Margalla Hills Islamabad, shade dried and subjected to cold maceration technique for extraction. Crude extract was partitioned into different fractions on polarity basis. Repeated normal and reverse phase column chromatography of ethyl acetate fraction supported by Thin Layer Chromatography have led to the isolation of pure single compound. Isolated compound was further analyzed towards structural elucidation using LC-MS, IR and NMR spectroscopy. Present finding will be a useful and novel addition in already known chemical components from Pistacia integerrima.

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“…The two HPLC systems used in succession separated the tested auxin-related compounds from IAA. Among those 4-Cl-IAA was of special interest as it has been reported to occur naturally preferentially in immature seeds (5,7) and has been found to be more active than IAA in the Avena straight-growth test (2). Therefore, 4-Cl-IAA might have interfered in earlier investigations using bioassays to determine IAA content during dormancy release in poorly purified extracts from seeds (see 'Introduction").…”

Section: Discussionmentioning

confidence: 99%

Levels of Endogenous Indole-3-acetic Acid in Achenes of Rosa rugosa during Dormancy Release and Germination

Tillberg¹

1984

Plant Physiol.

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ABSTRAC1Indole-3-acetic acid (IAA) in highly purified extracts of rose achenes (Rosa rugosa var rubra) was quantified by means of ion-pair reversephase high performance liquid chromatography with spectrofluorimetric detection. Changes in IAA content were determined during a 14-week 4°C stratifiation, which leads to dormancy breakage, and during subsequent germination at 17C. IAA was also determined in achenes stratified in parallel at 17°C, which does not induce release from dormancy. IAA decreased during the first 2 weeks of stratification both at 4C and at 17°C. IAA remained low during the remaning 12 weeks of stratification at 4°C, whereas it continued to decrease in achenes kept at 17C. An immediate increase in IAA during germination was followed by transients in the IAA level. The results suggest that IAA is without a regulating role in dormancy release although it seems to be involved in the germination process.Hormonal regulation of dormancy breakage and germination are believed to be affected by an interplay between endogenous promotors and inhibitors. As these two processes commonly overlap each other in time, it may be difficult to judge if the observed hormonal changes are linked to one or the other process. Rose achenes, however, seem to be a suitable study material, since the period during which embryo dormancy is broken is well separated from the subsequent germination period (16), which is blocked by germination inhibitors (8). In two recent studies from our laboratory, changes in the levels of cytokinins and ABA in achenes of Rosa rugosa have been reported. A transient increase of cytokinin-like substances appeared early during cold stratification and another during germination suggesting that they are involved in both processes (9), whereas ABA was shown to play only a passive role, if any, in the regulation of dormancy release and germination (16). The present report describes changes in IAA levels in the same material and discusses its role in these processes.There are reports suggesting that IAA is involved as an inhibitor in the regulation of dormancy whereas the effect of auxin on germination has long been in dispute. Decreases in the levels of auxins in apple seeds during a time course were strikingly similar to those of growth inhibitors during cold stratification (10 IAA diminishes both in cold and warm stratified embryos of Acer platanoides and have suggested that there is more direct relationship between IAA content and germination-growth than between IAA content and dormancy breakage (17). For these reasons, the possible role of IAA in these processes is controversial. The purpose of the present investigation was to determine the dynamics of IAA during cold stratification at 4°C and during germination induced by transfer to 17°C after a 14-week stratification period. Controls were conducted in parallel at 17C, which does not break dormancy. As preliminary experiments with the indolo-a-pyrone method gave low yields of IAA, a more sensitive method, HPLC with fluorescence detector, was a...

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Growth of Avena coleoptiles and pH drop of protoplast suspensions induced by chlorinated indoleacetic acids

Cited by 67 publications

References 15 publications

The effect of auxins (IAA and 4-Cl-IAA) on the redox activity and medium pH of Zea mays L. root segments

The effect of auxins (IAA and 4-Cl-IAA) on the redox activity and medium pH of Zea mays L. root segments

Isolation Studies From Stem Extract of Pistacia Integerrima Stew. Ex Brand

Levels of Endogenous Indole-3-acetic Acid in Achenes of Rosa rugosa during Dormancy Release and Germination

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