Growth of and competition between Neospora caninum and Toxoplasma gondii in vitro

Sundermann, Christine A.; Estridge, Barbara H.

doi:10.1016/s0020-7519(99)00114-9

Cited by 16 publications

(12 citation statements)

References 16 publications

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“…This is in agreement with Sundermann and Estridges (1999) who showed that the T. gondii RH strain multiplies faster than the N. caninum NC-1 strain in vitro.…”

Section: Discussionsupporting

confidence: 93%

Transplacental transmission in cattle: is Toxoplasma gondii less potent than Neospora caninum?

et al. 2011

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We compared the transplacental-transmission ability of Toxoplasma gondii and Neospora caninum in cattle. One uninfected pregnant heifer served as control, while three were inoculated with N. caninum K9WA strain and four with T. gondii RH strain at their midgestational period. Both infected groups showed clinical signs and antibodies either to N. caninum or T. gondii, while the control animal was normal. Two (50%) Toxoplasma dams aborted on days 6 and 11 postinoculation. T. gondii tachyzoites were found in various organs of those dams that had abortions but not in their fetuses. Two Neospora dams did not abort but gave birth to subclinically infected calves. The remaining two Toxoplasma dams and one from Neospora group became recumbent. Those two dams and their fetuses showed disseminated Toxoplasma DNA, but no Neospora DNA was found. Our findings suggest that maternal toxoplasmosis could be a cause of abortion and congenital toxoplasmosis in cattle, especially when they are infected by virulent strains.

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“…This is in agreement with Sundermann and Estridges (1999) who showed that the T. gondii RH strain multiplies faster than the N. caninum NC-1 strain in vitro.…”

Section: Discussionsupporting

confidence: 93%

Transplacental transmission in cattle: is Toxoplasma gondii less potent than Neospora caninum?

et al. 2011

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“…Our results were similar to the results previously obtained for the Nc-1 isolate, which displayed a lag period of 10-12 h and a Td of 14-15 h using human foreskin fibroblasts [40]. However, we must consider, as we did for the IRs, that the proliferation kinetics may have been influenced by the host cell lines used in these assays [39].…”

Section: Discussionsupporting

confidence: 88%

In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum

Regidor‐Cerrillo

Gómez-Bautista

Sodupe

et al. 2011

Vet Res

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In this study, we examined the in vitro invasion and proliferation capacities of the Nc-Liv and ten Spanish Neospora caninum isolates (Nc-Spain 1 H - Nc-Spain 10). The invasion rate was determined as the number of tachyzoites that completed their internalisation into MARC-145 cells at 2, 4, and 6 h post-inoculation (pi). The proliferation rate was evaluated by determining the doubling time during the exponential proliferation period. Significant differences in the invasion rates of these isolates were detected at 2 and 4 h pi (P < 0.0001, Kruskal-Wallis test). At 4 h pi, the Nc-Spain 4 H and Nc-Liv isolates displayed the highest, while the Nc-Spain 3 H and Nc-Spain 1 H isolates had the lowest invasion rates (by Dunn's test). Variations in the proliferation kinetics of these isolates were also observed. Between different isolates, the lag phase, which occurs before the exponential growth phase, ranged from 8 to 44 h, and the doubling time ranged from 9.8 to 14.1 h (P = 0.0016, ANOVA test). Tachyzoite yield, which combines invasion and proliferation data, was also assessed and confirmed marked differences between the highly and less prolific isolates. Interestingly, a direct correlation between the invasion rates and tachyzoite yields, and the severity of the disease that was exhibited by infected pregnant mice in previous works could be established for the isolates in this study (Spearman's coefficient > 0.62, P < 0.05). The results of this study may help us to explain the differences in the pathogenicity that are displayed by different isolates.

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“…In contrast to our data, Sundermann and Estridge (1999) compared invasion by RH and NC-1 isolates to human foreskin fibroblasts and found that 2 to 3-fold more NC-1 tachyzoites were intracellular compared with RH at 1 or 3 h p.i. One possible explanation for the difference between our results and theirs, is that T. gondii can display different growth dynamics depending on the type of cultured cell used (Hughes, Hudson and Fleck, 1986).…”

Section: Discussioncontrasting

confidence: 99%

Attachment and invasion ofToxoplasma gondiiandNeospora caninumto epithelial and fibroblast cell linesin vitro

2005

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Attachment and invasion of Toxoplasma gondii and Neospora caninum to a cat and a dog fibroblast cell line and 2 epithelial cell lines (a cat kidney and Vero) were compared in vitro using fluorescence antibody methodology. In addition, trypsin treatment of tachyzoites was used to determine whether protein molecules were essential to the process of invasion. The results show that both T. gondii and N. caninum invaded all 4 cell lines, and that pre-treatment of T. gondii tachyzoites with trypsin caused an increase in the ability of the parasite to invade these host cells. Furthermore T. gondii, in comparison to N. caninum, invaded all 4 cell lines at greater levels. The results here support the conclusion that both T. gondii and N. caninum have the ability to invade a variety of cell types including both dog and cat cells, and questions the utility of Vero cells as an appropriate host cell for in vitro studies on the biology of these taxa.

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Growth of and competition between Neospora caninum and Toxoplasma gondii in vitro

Cited by 16 publications

References 16 publications

Transplacental transmission in cattle: is Toxoplasma gondii less potent than Neospora caninum?

Transplacental transmission in cattle: is Toxoplasma gondii less potent than Neospora caninum?

In vitro invasion efficiency and intracellular proliferation rate comprise virulence-related phenotypic traits of Neospora caninum

Attachment and invasion ofToxoplasma gondiiandNeospora caninumto epithelial and fibroblast cell linesin vitro

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