Attachment and invasion ofToxoplasma gondiiandNeospora caninumto epithelial and fibroblast cell linesin vitro

Abstract

Attachment and invasion of Toxoplasma gondii and Neospora caninum to a cat and a dog fibroblast cell line and 2 epithelial cell lines (a cat kidney and Vero) were compared in vitro using fluorescence antibody methodology. In addition, trypsin treatment of tachyzoites was used to determine whether protein molecules were essential to the process of invasion. The results show that both T. gondii and N. caninum invaded all 4 cell lines, and that pre-treatment of T. gondii tachyzoites with trypsin caused an increase in the ability of the parasite to invade these host cells. Furthermore T. gondii, in comparison to N. caninum, invaded all 4 cell lines at greater levels. The results here support the conclusion that both T. gondii and N. caninum have the ability to invade a variety of cell types including both dog and cat cells, and questions the utility of Vero cells as an appropriate host cell for in vitro studies on the biology of these taxa.

Seroprevalence of Toxoplasma gondii in wild kangaroos using an ELISA

“…Antigen for the ELISA was prepared from the Type I RH strain of T. gondii tachyzoites grown in Vero cell culture adapted from [35]. After infected cell cultures were harvested, tachyzoites were purified from the Vero cells by shearing through a 30G needle then filtration with a 5μm syringe filter.…”

Congenital Transmission of Apicomplexan Parasites: A Review

“…Neospora caninum can actively invade a large variety of nucleated cells in vitro and in vivo conditions (Naguleswaran et al, 2002;Hemphill et al, 2004;Lei et al, 2005); its mechanisms are very similar to those proposed for T. gondii, where a first stage of low-affinity adhesion and a second stage of firm apical attachment can be observed (Hemphill et al, 2004;Carruthers and Boothroyd, 2007).…”

Virulência e multiplicação de isolados de Toxoplasma gondii da região central do Rio Grande do Sul

“…As células de linhagem Vero, são comumente utilizadas para multiplicação e manutenção laboratorial de T. gondii (De Meerschman et al 2002, Saadatnia et al 2010, sendo o tipo celular escolhido para avaliação deste trabalho. Taquizoítos de T. gondii, conhecidamente possuem a capacidade de invadir diversos tipos de células nucleadas, em diferentes espécies animais (Miller et al 1972, Canfield et al 1990, Inskeep 2nd et al 1990, Hartley & Dubey 1991, Dubey et al 1998, Evans et al 1999, Chatterton et al 2002, Lei et al 2005 e o comportamento referente a quantidade de multiplicação pode estar ligado a uma maior adaptação do tipo de isolado ou tipo celular utilizado para sua manutenção (Diab & El-Bahy 2008).…”