Growth of a bacterial consortium on triclosan

Abstract

Triclosan is a polychlorinated hydroxy diphenylether that has been widely used as an antimicrobial compound. An enrichment using triclosan as a sole source of carbon and energy yielded a consortium of bacteria capable of growing on this compound. The dichloro ring was partially mineralized, resulting in the conversion of approximately 35% of the [(14)C]triclosan to [(14)C]CO(2). Use of molecular fingerprinting techniques and 16S rDNA cloning and sequencing aided in the identification and eventual isolation of an auxotrophic Sphingomonas-like organism, strain Rd1, which was able to partially mineralize triclosan when grown on complex media.

New insights into a bacterial metabolic and detoxifying association responsible for the mineralization of the thiocarbamate herbicide molinate

“…Feigel & Knackmuss, 1993;Pelz et al, 1999) or supply of nutritional requirements (e.g. Hay et al, 2001;Sorensen et al, 2002).…”

Global analysis of candidate genes important for fitness in a competitive biofilm using DNA-array-based transposon mapping

“…Blue-white screening was performed on LB Amp plates containing 40 mg X-Gal l 21 (International Biotechnologies). Minimal salts medium (MSM) (Hay et al, 2001) supplemented with 0?2 % (w/v) glucose, with or without 0?2 % (w/v) Casamino acids, was also used. Table 2 lists the oligomers used for PCR and adaptamer synthesis, which were purchased from Integrated DNA Technologies.…”

“…Table 2 lists the oligomers used for PCR and adaptamer synthesis, which were purchased from Integrated DNA Technologies. PCR amplification was done using ThermoStart Master mix (ABgene) and a modified touchdown thermoprofile (Hay et al, 2001), unless otherwise specified. Briefly, the initial denaturation was 15 min at 95 uC, followed by 10 cycles with denaturation at 95 uC for 45 s, annealing at 65 uC for 15 s, and extension at 70 uC for 60 s. The annealing temperature was decreased by 1 uC per cycle for the first 10 cycles and then maintained at 55 uC for the remaining 20 cycles.…”

Biodegradation, Biotransformation, and Biocatalysis (B3)

“…Anthony Hay (Cornell University) enriched for triclosan-degrading microorganisms by using samples from a municipal wastewater treatment plant. A stable bacterial consortium consisting of six microorganisms was capable of using triclosan as the sole source of carbon and energy, removing over 90% of the supplied triclosan with 35% mineralization of the compound (39).…”