Growth Medium Optimization for Biomass Production of a Probiotic Bacterium,Lactobacillus rhamnosus ATCC 7469

Abstract

This study was undertaken to determine the glucose, yeast extract, tryptone and Tween 80 concentrations in a cultivation medium for optimal biomass production of a probiotic bacterium, Lactobacillus rhamnosus ATCC 7469. Using response surface methodology, a central composite rotatable design was applied to allocate treatment combinations. A polynomial regression model containing linear, quadratic, cubic and quartic terms was used for analysis of the experimental data. Effects involving all four factors evaluated were found to be significant and the strongest effect was given by tryptone concentration. Estimated optimum conditions of the factors for the growth of L. rhamnosus were as follows: glucose = 4.4% (w/v), yeast extract = 6.0% (w/v), tryptone = 6.0% (w/v) and Tween 80 = 1.1 mL/L. The optimum‐point medium gave higher counts of viable cells, specific growth rate and biomass productivity than de Man, Rogosa and Sharpe and the center‐point media. Practical Applications With increasing demand for food products containing probiotic microorganisms from health‐conscious consumers, there arises a need to establish effective fermentation techniques for probiotic biomass production. Medium formulation, fermentation parameters and fermentor design play important roles to ensure optimal results. This study highlights a statistical approach to optimizing medium formulation for a probiotic lactic acid bacteria strain, which is known for its high nutritional demands. The optimal medium formulation obtained from this study can be used for improving Lactobacillus rhamnosus fermentation as it is capable of producing more viable cells than de Man, Rogosa and Sharpe medium, which is generally recognized and used as a suitable medium for lactic acid bacteria cultivation.

Deproteinated Cheese Whey Medium for Biomass Production of Probiotic Lactobacillus helveticus MTCC 5463

“…0.95% (w/v) for each, were slightly higher than the level of supplementation reported by Masuda et al, (2006). On the other hand, the level optimized for different media constituents in the present study are found to be less than the data reported by other researchers (Liew et al, 2005;Bevilacqua et al, 2008;Chang and Liew, 2012). The difference found in the studies regarding level of YE and PP to obtain maximum biomass production from lactobacilli may be mainly due to addition of other ingredients such as glucose, meat extract, tryptone, casein or other protein hydrolysates and salts along with YE and PP which may provide growth nutrients to lactobacilli and thereby reduces the requirement of YE and PP.…”

Wheat and Sugar Beet Coproducts for the Bioproduction of 3-Hydroxypropionic Acid by Lactobacillus reuteri DSM17938

“…The method enables to obtain a large amount of data from a reduced number of experiments and to study the influence of different input variables on a specific response, including the interactions between the studied factors. RSM has been successfully used for the optimization of culture media for different microorganisms including lactobacillus species [15][16][17][18][19].…”

Probiotics media: significance, challenges, and future perspective - a mini review

“…Probiotic lactobacilli are nutritionally fastidious organisms. Therefore, their viability and growth activity are commonly influenced by growth factors such as medium formulations, pH, temperature, and others (Chang & Liew 2013;Terpou et al 2017;Dang et al 2021). The less cell mass production of lactic acid bacteria during its industrial production using a bioreactor is attributed to the reduced growth rate of cells and high production of lactate (Du Toit et al 2013).…”