Growth irradiance affects the photoprotective mechanisms of the resurrection angiosperm Haberlea rhodopensis Friv. in response to desiccation and rehydration at morphological, physiological and biochemical levels

Rapparini, Francesca; Neri, Luisa; Mihailova, Gergana; Petkova, S.; Georgieva, Katya

doi:10.1016/j.envexpbot.2015.01.007

Cited by 24 publications

(25 citation statements)

References 68 publications

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“…The observed decrease in F v / F m upon desiccation of R. myconi leaves was much more moderate than what has been reported for bryophytes or lichens, but is consistent with values reported for other resurrection plants; i.e. F v / F m values >0.3 were measured in dry leaves of R. serbica (Gashi et al ), R. nathaliae (Fernández‐Marín et al ), H. rhodopensis (Rapparini et al ) or Craterostigma pumilum (Charuvi et al ); and F v / F m values >0.4 were obtained in R. nathaliae (Gashi et al ) or in dry fronds of the desiccation tolerant‐fern Hymenoglossum cruentum (Flores‐Bavestrello et al ). The drop in F v / F m shown by R. myconi was accompanied by synthesis of Z, even in the dark (Fig.…”

Section: Discussionsupporting

confidence: 88%

First evidence of freezing tolerance in a resurrection plant: insights into molecular mobility and zeaxanthin synthesis in the dark

Fernández‐Marín

Neuner

Kuprian

et al. 2018

Physiologia Plantarum

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The photoprotective mechanisms of desiccation tolerance and freezing tolerance and their relation to molecular mobility (cell vitrification) were assessed in a single model: the exceptional subalpine and resurrection plant Ramonda myconi. Dehydrated leaves showed a drop in maximal photochemical efficiency of PSII (Fv/Fm) accompanied by synthesis of zeaxanthin (Z), even in the dark, which was limited by cell vitrification after complete desiccation. The recovery of Fv/Fm after a severe drying treatment (7 days at 50% relative humidity) confirmed the tolerance of R. myconi leaves to desiccation. In winter, R. myconi plants showed a highly dynamic component of photoinhibition. Interestingly, the potential activity of the enzyme violaxanthin de‐epoxidase (VDE) occurred at −7°C, below the freezing temperature range of the leaves (−2 ± 2°C) and even in the dark. This suggests that, in nature, the enzyme can still be active in frozen leaves, as long as they are above the glass transition temperature. The drop in Fv/Fm and increase in Z were reversible upon rehydration and thawing, respectively, but were not perfectly matched, suggesting that both Z‐independent and Z‐dependent forms of sustained dissipation are occurring. Overall, our data reinforce the light‐independent activity of the VDE enzyme under stress and suggest that Z‐accumulation could occur in darkness in a scenario when temperatures drop dramatically in the night under natural conditions.

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Section: Discussionsupporting

confidence: 88%

First evidence of freezing tolerance in a resurrection plant: insights into molecular mobility and zeaxanthin synthesis in the dark

Fernández‐Marín

Neuner

Kuprian

et al. 2018

Physiologia Plantarum

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“…By contrast, J flu‐Chl exhibited relatively high values (Fig. 4c), indicating a restoration of the electron transport chain before the carbon fixation mechanism, as has been described for H. rhodopensis , 1 d after rehydration (Rapparini et al , 2015).…”

Section: Discussionsupporting

confidence: 61%

“…The photosynthetic pigment profile has very rarely been studied in desiccation‐tolerant species (Kranner et al , 2002; Georgieva et al , 2009, 2017b; Beckett et al , 2012; Rapparini et al , 2015). Several additional works conducted spectrophotometric measurements and some followed obsolete procedures (Markovska et al , 1994; Deng et al , 2003), as recently pointed out (Fernández‐Marín et al , 2015; Esteban et al , 2018; Porra & Scheer, 2019).…”

Section: Discussionmentioning

confidence: 99%

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

et al. 2020

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Summary Resurrection plants recover physiological functions after complete desiccation. Almost all of them are native to tropical warm environments. However, the Gesneriaceae include four genera, remnant of the past palaeotropical flora, which inhabit temperate mountains. One of these species is additionally freezing‐tolerant: Ramonda myconi. We hypothesise that this species has been able to persist in a colder climate thanks to some resurrection‐linked traits. To disentangle the physiological mechanisms underpinning multistress tolerance to desiccation and freezing, we conducted an exhaustive seasonal assessment of photosynthesis (gas exchange, limitations to partitioning, photochemistry and galactolipids) and primary metabolism (through metabolomics) in two natural populations at different elevations. R. myconi displayed low rates of photosynthesis, largely due to mesophyll limitation. However, plants were photosynthetically active throughout the year, excluding a reversible desiccation period. Common responses to desiccation and low temperature involved chloroplast protection: enhanced thermal energy dissipation, higher carotenoid to Chl ratio and de‐epoxidation of the xanthophyll cycle. As specific responses, antioxidants and secondary metabolic routes rose upon desiccation, while putrescine, proline and a variety of sugars rose in winter. The data suggest conserved mechanisms to cope with photo‐oxidation during desiccation and cold events, while additional metabolic mechanisms may have evolved as specific adaptations to cold during recent glaciations.

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“…Fv/Fm is a normalized ratio created by dividing variable ChlF by maximum ChlF and represents the maximal efficiency of PSII to photochemistry (Baker, 2008). While Fv/Fm values in the range of 0.79–0.84 are regarded optimal for many plant species, decreased values are frequently observed when plants are exposed to various stress conditions such as high light, chilling, and salinity (Jamil et al, 2007; Na et al, 2014; Rapparini et al, 2015). In this study, it was noted that strawberry plants grown under BF condition of low light and low temperature showed higher Fv/Fm ratios than those plants grown under UT, UF, and BT conditions (Figures 2, 3).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, environmental factors such as light (Biswal and Biswal, 1999; Zivcak et al, 2014; Rapparini et al, 2015) and temperature (Lafta and Lorenzen, 1995; Gupta et al, 2016) have a significant impact on the growth and development of plants. Low temperature (LT) is one of the most significant abiotic stresses limiting plant photosynthesis (Ashraf and Harris, 2013) and severely retards plant growth and function (Gupta et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Correlation between Strawberry (Fragaria ananassa Duch.) Productivity and Photosynthesis-Related Parameters under Various Growth Conditions

2016

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In the present study, we investigated changes in chlorophyll fluorescence, photosynthetic parameters and fruit yields, as well as fruit phytochemical accumulation of strawberry (Fragaria ananassa Duch.) that had been cultivated in a greenhouse under different combinations of light intensity and temperature. In plants grown with low light (LL) photosystem II chlorophyll fluorescence was found to increase as compared with those grown under high light (HL). When strawberry plants were grown with temperature higher than 5°C in addition to LL, they showed decrease in non-photochemical quenching (NPQ), photochemical quenching (qP), as well as chlorophyll fluorescence decrease ratio (RFd) when compared with other combinations of light and temperature. Moreover, fruit yield of strawberry was closely correlated with chlorophyll fluorescence-related parameters such as NPQ, qP, and RFd, but not with the maximum efficiency of PS II (Fv/Fm). Although plant groups grown under different combinations of light and temperature showed almost comparable levels of photosynthesis rates (Pr) when irradiated with low-intensity light, they displayed clear differences when measured with higher irradiances. Plants grown under HL with temperature above 10°C showed the highest Pr, in contrast to the plants grown under LL with temperature above 5°C. When the stomatal conductance and the transpiration rate were measured, plants of each treatment showed clear differences even when analyzed with lower irradiances. We also found that fruit production during winter season was more strongly influenced by growth temperature than light intensity. We suggest that fruit productivity of strawberry is closely associated with chlorophyll fluorescence and photosynthesis-related parameters during cultivation under different regimes of temperature and light.

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Growth irradiance affects the photoprotective mechanisms of the resurrection angiosperm Haberlea rhodopensis Friv. in response to desiccation and rehydration at morphological, physiological and biochemical levels

Cited by 24 publications

References 68 publications

First evidence of freezing tolerance in a resurrection plant: insights into molecular mobility and zeaxanthin synthesis in the dark

First evidence of freezing tolerance in a resurrection plant: insights into molecular mobility and zeaxanthin synthesis in the dark

Born to revive: molecular and physiological mechanisms of double tolerance in a paleotropical and resurrection plant

Correlation between Strawberry (Fragaria ananassa Duch.) Productivity and Photosynthesis-Related Parameters under Various Growth Conditions

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