Growth inhibitory effect of an injectable hyaluronic acid–tyramine hydrogels incorporating human natural interferon-α and sorafenib on renal cell carcinoma cells

Ueda, Kosuke; Akiba, Jun; Ogasawara, Satoshi; Todoroki, Keita; Nakayama, Masamichi; Sumi, Akiko; Kusano, Hironori; Sanada, Sakiko; Suekane, Shigetaka; Xu, Keming; Bae, Ki Hyun; Kurisawa, Motoichi; Igawa, Tsukasa; Yano, Hirohisa

doi:10.1016/j.actbio.2015.10.024

Cited by 47 publications

(33 citation statements)

References 30 publications

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“…We found that AL–HA–Tyr significantly reduced the ratios of Ki67- and VEGF-A positive cells in transplanted tumors and significantly prolonged the survival of tumor-bearing mice. As reported in other studies, HA–Tyr hydrogels enhanced therapeutic effectiveness and allowed for continuous release (Xu et al., 2013 , 2015 ; Ueda et al., 2016 ; Tang et al., 2019 ).…”

Section: Discussionsupporting

confidence: 64%

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

et al. 2020

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This study was conducted to determine the antitumor effects and ability of an anlotinib (AL) hydrogel (AL–HA–Tyr) to reduce toxicity in a mouse model of Lewis lung cancer (LLC). We constructed a drug carrier system for AL, verified its effectiveness and systemic safety, and provided a preliminary experimental foundation for clinical carrier transformation. AL–HA–Tyr was prepared by encapsulating AL with hyaluronic acid–tyramine (HA–Tyr) conjugates. Colony and tube formation assays showed that AL–HA–Tyr restrained the proliferation of human umbilical vein endothelial cells (HUVECs) and LLC cells, respectively, in vitro , and that AL exerted significant anti-angiogenesis and anti-tumor effects. The invasion and migration of HUVECs and LLC cells were efficiently suppressed by AL according to transwell assays. HUVEC and LLC cell-cycle and apoptosis analysis clarified the direct anti-tumor effects of AL–HA–Tyr. Mice engrafted with LLC cells in vivo were administered oral saline, oral AL, or an intratumoral injection of HA–Tyr or AL–HA–Tyr. The results showed that AL–HA–Tyr obviously reduced visceral toxicity and decreased Ki67 and VEGF-A expression in tumor cells compared with AL. Furthermore, AL–HA–Tyr significantly prolonged the survival of tumor-bearing mice. Overall, AL–HA–Tyr enhanced antitumor effects and reduced toxicity in the LLC model. It provided a foundation for the clinical transformation of drug carrier systems.

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Section: Discussionsupporting

confidence: 64%

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

et al. 2020

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“…The first, HA-Tyr hydrogel had been previously investigated as a biocompatible drug delivery material. 41 The effect of crosslinking density on the behaviour of the HA-Tyr hydrogels was investigated based on the degree of Tyr substitution of HA (1 and 5%). The second, a pNIPAAM-based gel was chosen because it possesses thermoresponsive/collapsing property and was expected to sustain the release of the loaded drug.…”

Section: Preparation Of Hydrogelsmentioning

confidence: 99%

Antibody loaded collapsible hyaluronic acid hydrogels for intraocular delivery

Egbu

Brocchini

Khaw

et al. 2018

European Journal of Pharmaceutics and Biopharmaceutics

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Injectable gels have the potential to encapsulate drugs for sustained release of protein therapeutics for use in the eye. Hyaluronic acid (HA) is a biodegradable clinically used material and poly N-isopropylacrylamide (pNIPAAM) is a stimuli responsive polymer that can display a lower critical solution temperature (LCST) at physiological conditions. Two gel systems incorporating HA were prepared in the presence of the antibody infliximab (INF): i) 1% and 5% tyramine-substituted HA (HA-Tyr) was enzymatically crosslinked in the presence of INF to form HA-Tyr-INF and ii) NIPAAM was chemically crosslinked in the presence of HA and INF with 1 and 3% poly(ethylene glycol) diacrylate (PEGDA) to form PEGDA-pNIPAAM-HA-INF. The PEGDA-pNIPAAM-HA-INF hydrogels displayed LCSTs at temperatures ranging from 31.4 ± 0.2 to 35.7 ± 0.3 °C. Although all the gels prepared were injectable, INF-loaded gels with lower crosslinking density (1% PEGDA-pNIPAAM-HA and 1% HA-Tyr) showed lower elastic (G') and viscous (G″) moduli compared to higher crosslinked gels (3% PEGDA-pNIPAAM-HA-INF and 5% HA-Tyr-INF) resulting in differences in swelling ratio (SR). Moduli may be correlated with overall stiffness of the gel. All hydrogels demonstrated sustained release of INF in a two-compartment in vitro outflow model of the human eye called the PK-Eye. The 1% PEGDA-pNIPAAM-HA-INF hydrogel displayed the slowest release (24.9 ± 0.4% INF release by day 9) in phosphate buffered saline (PBS, pH 7.4), which is a better release profile than the free drug alone (tested under the same conditions). These results suggest that PEGDA-pNIPAAM-HA has potential for the continued development of formulations to prolong the intraocular release of proteins.

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“…Hyaluronic acid–tyramine-based hydrogels have been used to deliver IFN-α to the injection site and to inhibit tumor proliferation via the coadministration of sorafenib [ 183 ]. In addition, a hyaluronic acid-pluronic F-127 hydrogel was used to prepare black phosphorus quantum dot nanovesicles (BPQD-CCNVs), GM-CSF, and LPS coloaded systems.…”

Section: Novel Biomaterials For Cancer Immunotherapymentioning

confidence: 99%

Advanced biomaterials for cancer immunotherapy

et al. 2020

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Immunotherapy, as a powerful strategy for cancer treatment, has achieved tremendous efficacy in clinical trials. Despite these advancements, there is much to do in terms of enhancing therapeutic benefits and decreasing the side effects of cancer immunotherapy. Advanced nanobiomaterials, including liposomes, polymers, and silica, play a vital role in the codelivery of drugs and immunomodulators. These nanobiomaterial-based delivery systems could effectively promote antitumor immune responses and simultaneously reduce toxic adverse effects. Furthermore, nanobiomaterials may also combine with each other or with traditional drugs via different mechanisms, thus giving rise to more accurate and efficient tumor treatment. Here, an overview of the latest advancement in these nanobiomaterials used for cancer immunotherapy is given, describing outstanding systems, including lipid-based nanoparticles, polymer-based scaffolds or micelles, inorganic nanosystems, and others.

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Growth inhibitory effect of an injectable hyaluronic acid–tyramine hydrogels incorporating human natural interferon-α and sorafenib on renal cell carcinoma cells

Cited by 47 publications

References 30 publications

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

Intratumoral injection of anlotinib hydrogel enhances antitumor effects and reduces toxicity in mouse model of lung cancer

Antibody loaded collapsible hyaluronic acid hydrogels for intraocular delivery

Advanced biomaterials for cancer immunotherapy

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