Growth factors and their receptors derived from human amniotic cells in vitro

Grzywocz, Zofia; Pius-Sadowska, Ewa; Kłos, Patrycja; Gryzik, Marek; Wasilewska, Danuta; Aleksandrowicz, Barbara; Dworczynska, Malgorzata; Sabalińska, Stanisława; Hoser, Grazyna; Machalinski, Bogdan; Kawiak, Jerzy

doi:10.5603/fhc.2014.0019

Cited by 43 publications

(33 citation statements)

References 30 publications

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“…The proliferation, migration and angiogenic properties of endothelial cells are important in the revascularization of ischemic tissue and the reperfusion of myocardial infarction. While recent studies have revealed the angiogenic properties of human amniotic membrane and mesenchymal stem cells (16,21), information concerning hAECs is rare, and comparative studies of the biological effects between hAECs and hAMSCs are lacking. Therefore, in the present study, we reported that amniotic cells possess high biological potential for endothelial cells and we compared the differences in the cellular function and biological properties between these cells.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells

Fang

Lang

et al. 2017

International Journal of Molecular Medicine

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In vivo studies have shown that amnion-produced growth factors participate in many diseases that involve angiogenesis, re-epithelialization and immunomodulation. Although human amniotic epithelial cells (hAECs) and human amniotic mesenchymal stem cells (hAMSCs) can be obtained from amniotic membranes, there is little information regarding their biological differences. The aim of the present study was to isolate and characterize cells from human amnions, to investigate the biological potential and behavior of these cells on the function of endothelial cells in vivo and in vitro and to examine variations in the expression profile of growth factors in different human amnion-derived cell types. Amnion fragments were enzymatically digested into two cell fractions, which were analyzed by mesenchymal and epithelial cell markers. Human aortic endothelial cells (hAoECs) were cultured with conditioned medium (CdM) collected from hAECs or hAMSCs. We used scratch and Transwell assays to evaluate migration ability; Cell Counting Kit-8 (CCK-8) and cell cycle analysis to evaluate proliferation ability; and a Matrigel tube formation assay to evaluate angiogenesis ability. To detect expression of angiogenesis-related genes, qPCR and enzyme-linked immunosorbent assay (ELISA) analyses were conducted. As stem cells, hAECs and hAMSCs all expressed the stem cell markers SSEA-4, OCT-4 and SOX-2. CdM obtained from hAECs promoted cell migration; CdM obtained from hAMSCs promoted cell proliferation; CdM obtained from hAECs and hAMSCs both promoted angiogenesis in hAoECs. Amnion-derived cells secreted significant amounts of angiogenic factors including HGF, IGF-1, VEGF, EGF, HB-EGF and bFGF, although differences in the cellular expression profile of these soluble factors were observed. Our results highlight that human amniotic epithelial and mesenchymal stem cells, which showed differences in their soluble factor secretion and angiogenic functions, could be ideal cell sources for regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells

Fang

Lang

et al. 2017

International Journal of Molecular Medicine

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“…Signaling through this pathway results in a potent chemotactic and mitogenic response seen in pericyte recruitment, both during development 69, 70, and following tissue injury 71, 72. In cell culture, it has been reported that amniotic epithelial cells produce PDGF‐B 73, 74, and therefore, could potentially serve as a stimulus for perivascular migration in vivo. Whether such a response does truly occur during development, however, has never been investigated.…”

Section: Are Wharton's Jelly Mscs Perivascular In Origin?mentioning

confidence: 99%

Concise Review: Wharton's Jelly: The Rich, but Enigmatic, Source of Mesenchymal Stromal Cells

Davies

Walker

Keating

2017

Stem Cells Translational Medicine

153

138

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The umbilical cord has become an increasingly used source of mesenchymal stromal cells for preclinical and, more recently, clinical studies. Despite the increased activity, several aspects of this cell population have been under‐appreciated. Key issues are that consensus on the anatomical structures within the cord is lacking, and potentially different populations are identified as arising from a single source. To help address these points, we propose a histologically based nomenclature for cord structures and provide an analysis of their developmental origins and composition. Methods of cell isolation from Wharton's jelly are discussed and the immunophenotypic and clonal characteristics of the cells are evaluated. The perivascular origin of the cells is also addressed. Finally, clinical trials with umbilical cord cells are briefly reviewed. Interpreting the outcomes of the many clinical studies that have been undertaken with mesenchymal stromal cells from different tissue sources has been challenging, for many reasons. It is, therefore, particularly important that as umbilical cord cells are increasingly deployed therapeutically, we strive to better understand the derivation and functional characteristics of the cells from this important tissue source. Stem Cells Translational Medicine 2017;6:1620–1630

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“…Additionally, transplanted hAECs may also secret anti-inflammatory factors via a paracrine pathway, and promoting the secretions of growth factors may lead to a local microenvironment more conducive for follicle growth and development. Recently, research have reported that cultured human amnion secreted various growth factors, such as fibroblast growth factor-6, neurotrophin-4, vascular endothelial growth factor receptor-3, macrophage colony-stimulating factor receptor and heterodimer of platelet derived growth factors AB, which may have effects within regenerating damaged tissue [ 35 ]. While the current study describes an interesting phenomenon and hints at a partial mechanism for hAEC-induced ovarian regeneration after chemotherapy-induced POF, there is still much to be understood about the effect of cytokines secreted by hAEC on GCs and follicular development of chemoablated mice using the co-culture system.…”

Section: Discussionmentioning

confidence: 99%

Human amniotic epithelial cells inhibit granulosa cell apoptosis induced by chemotherapy and restore the fertility

Zhang

Yao

et al. 2015

Stem Cell Res Ther

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IntroductionPremature ovarian failure and insufficiency are significant long-term side-effects of chemotherapy for female cancer patients. Recently, stem cell transplantation has been identified as a promising treatment for premature ovarian failure and insufficiency. We have previously demonstrated that human amniotic epithelial cells (hAECs) migrate into injured tissue and promote the recovery of ovarian function in chemoablated mice. However, the molecular mechanism guiding this process remains unclear.MethodsTo further investigate the effect of hAECs on chemotherapy-induced apoptosis, cultured primary hAECs were injected intravenously into mice treated with cyclophosphamide and busulphan. Apoptosis of granulosa cells was observed by TUNEL staining, and apoptosis-related gene expression was performed on ovarian tissue by real-time PCR and Western blot 7 days after hAEC transplantation. Additionally, the ovarian function and fertility of mice were assessed via counts of follicles and mating experiments at 4 weeks after hAEC transplantation.ResultshAECs significantly inhibited tumor necrosis factor-alpha-mediated granulosa cell apoptosis induced by chemotherapeutics and reduced the inflammatory reaction in ovaries at 7 days after transplantation. In addition, 4 weeks after transplantation, hAECs promoted the development of follicles and increased the number of cumulus oocyte complexes in chemoablated mice. Furthermore, hAECs improved ovarian mass and increased the number of follicles compared to those of the chemoablated group, and hAEC transplantation partially rescued the fertility of chemoablated mice.ConclusionshAEC transplantation promotes ovarian function by inhibiting tumor necrosis factor-alpha-mediated cell apoptosis and reducing inflammation in chemotherapy-induced premature ovarian failure. These results suggest a potential molecular mechanism for the effective therapy of hAEC transplantation in chemotherapy-induced premature ovarian failure and insufficiency.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-015-0148-4) contains supplementary material, which is available to authorized users.

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Growth factors and their receptors derived from human amniotic cells in vitro

Cited by 43 publications

References 30 publications

Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells

Comparison of the proliferation, migration and angiogenic properties of human amniotic epithelial and mesenchymal stem cells and their effects on endothelial cells

Concise Review: Wharton's Jelly: The Rich, but Enigmatic, Source of Mesenchymal Stromal Cells

Human amniotic epithelial cells inhibit granulosa cell apoptosis induced by chemotherapy and restore the fertility

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