Growth factors accelerate epithelial repair in sheep trachea

Abstract

Toxic gases and fumes have been shown to be injurious to the upper airways. Repair of this injury involves proliferation and migration of surviving nonciliated cells, followed by differentiation to a normal phenotype. Because recent results suggested that growth factors could improve the outcome of an airway injury, we undertook this study to determine the efficacy of these materials as an initial treatment to accelerate the healing process. In 24 anesthetized sheep, a portion of the trachea was exposed to smoke from smouldering cotton cooled to 37 degrees C. Twelve received aerosolized epidermal growth factor plus platelet derived growth factor, while twelve received placebo. At 10 days after injury, nonciliated and ciliated cells were totally absent in the injured trachea receiving the placebo. In animals receiving growth factors, nonciliated and ciliated cells, however, were present (56% and 31% of uninjured trachea, respectively). At 13 days after injury, nonciliated and ciliated cell counts in those receiving placebo were 67% and 33% of uninjured, respectively. In sheep receiving growth factors, tracheal nonciliated and ciliated cell counts had increased to 105% and 64% of uninjured trachea, respectively. We conclude that growth factors therapy after airway injury stimulates cell proliferation and differentiation, and this therapeutic intervention to accelerate the repair process in acute airway injury is an approach applicable to humans.

Epidermal growth factor (EGF) and EGF receptor in hypospadias

“…EGF has been reported to play a major role in epithelial The differentiation of the mammalian reproductive tract into the male phenotype depends on the presence of regeneration and wound healing; it has been shown to accelerate corneal wound healing and healing of the functional testes, secreting testosterone. Of significant interest are the recent reports of Gupta et al [7,8] who intestinal mucosa and respiratory epithelial tracts in experimental animals [9][10][11][12][13][14][15][16][17]. The inadequate expression found that the masculinization of the genitourinary tract may be mediated by EGF.…”

“…EGF plays an essential role in the normal regeneration had penoscrotal hypospadias, who had undergone two previous procedures complicated by fistula formation. and healing of epithelial surfaces, including respiratory, gastrointestinal, genitourinary and corneal epithelia [10][11][12][13][14][15][16][17]. EGF receptor (EGFR) has been identified on Immunohistochemistry cells of various origin, including fibroblasts, corneal cells, breast cells and cells derived from some tumours Tissues were collected in normal saline, fixed in 10% formalin for 24 h and embedded in paraffin wax.…”

Induction of the epidermal growth factor receptor and its ligands in nasal epithelium by ozone

“…19 EGF also promotes cell survival and can protect against smoke-induced injury in vivo. 20 The mechanisms underlying the stress/repair responses after ozone exposure in vivo are not well understood. In previous studies we have shown that nasal epithelium co-expresses 3 members of the c-erbB family (EGFR, cerbB2, and c-erbB3).…”

Réparation et régénération de l’épithélium respiratoire

“…De plus, Barrow et al ont suggéré que l'EGF et le PDGF (platelet-derived growth factor) favorisent la régénération de l'épithélium respiratoire en stimulant la prolifération et la différenciation des cellules épithéliales respiratoires [38]. L'HGF (hepatocyte growth factor), le KGF (keratinocyte growth factor) et les IL-1α et β régulent la migration cellulaire, mais peuvent également avoir une action mitogénique [39][40][41].…”