Growth Factor Delivery to a Cartilage-Cartilage Interface Using Platelet-Rich Concentrates on a Hyaluronic Acid Scaffold

Titan, Ashley L.; Schär, Michael; Hutchinson, Ian D.; Demange, Marco Kawamura; Chen, Tony; Rodeo, Scott A.

doi:10.1016/j.arthro.2019.12.004

Cited by 14 publications

(10 citation statements)

References 44 publications

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“…In simpler terms, immunogenicity refers to the ability of a substance to initiate a cell-mediated immune response. To date, there is limited research on the role of platelet concentrates as non-autogenous biological additives 35,36 . Our experiment examined the synergetic effect of L-PRF on the proliferation of non-autogenous SCs.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors

Wang

Mudalal

Sun

et al. 2020

Sci Rep

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This study evaluates the use of L-PRF as an autologous scaffold in nerve regeneration, and Schwann cells (SCs) proliferation and secretion of neurotrophic factors and its anti-inflammatory effect on SC Porphyromonas Gingivalis-Lipopolysaccharide (PG-LPS)-induced inflammatory responses in vitro. SEM was done to investigate various features of L-PRF. L-PRF-extracts was used to investigate the release of growth factors and treatment of SCs line. ELISA was applied to examine the release of IGF-1. The proliferative effect of L-PRF on SCs was assessed with CCK-8 assay. The effect of L-PRF on the mRNA and protein expression of SC neurotrophic factors were analyzed by RT-qPCR and ELISA. CCK-8 assay and RT-qPCR were used to determine the required concentration and the action time of PG-LPS before the anti-inflammatory effect of L-PRF was determined by measuring the changes in IL-1β, IL-6, and TNF-a with RT-qPCR and ELISA. There are different features in L-PRF. Fourteen days was sufficient to release adequate GF. The mRNA expressions of the pro-inflammatory cytokines were notably raised by PG-LPS in 3-hours treatment. L-PRF can increase SC proliferation, neurotrophic factors secretion, and suppress SC PG-LPS-induced inflammatory responses in vitro. L-PRF has the potential as an autologous biological additive for peripheral nerve regeneration in the event of nerve inflammation and injuries. Recently, dental implantation has become the preferred option to replace missing teeth. However, patients who undergo implant treatment frequently suffer from severe postsurgical complications, especially nerve injury which can cast negative impacts on patients' quality of life and work 1. Clinically, nerve injury is characterized by the sensory and functional disorder in the dominant area, such as alteration in the sensation of the mucosa, lower lip, and chin and the feeling can range from slight numbness to complete anesthesia 2,3. At present, the solution for nerve injury includes intensive treatment protocols such as medications and physical therapy. Generally speaking, the first-line treatment for nerve injury combines both pharmacological intervention and physiotherapy 4. Medication used for nerve injury includes oral steroids to resolve neuritis and edema, vitamin B12 for the regeneration of nerve terminals, and adenosine triphosphate (ATP) to encourage blood flow via vasodilation 5. Physiotherapy such as the use of laser and hot pack treatment is also useful for vasodilation to increase the blood flow 5. However, Kim et al. 4 reported that these therapies are not full proof in providing positive outcomes in the treatment of nerve injury. After medication and physiotherapy for inferior alveolar nerve injury, as high as 7 in 10 patients reported no improvement in sensation. In other words, they still experience the same level of dysesthesia.

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Section: Discussionmentioning

confidence: 99%

The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors

Wang

Mudalal

Sun

et al. 2020

Sci Rep

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“…Combinations of HA with glucocorticoid or platelet-rich plasma has been used in the treatment of OA. Titan et al [ 40 ] used platelet-rich concentrate in combination with HA in bovine cartilage to enhance cartilage injury repair. Smith et al [ 41 ] reported that the intraarticular injection comprising corticosteroid and HA exhibited better pain relief than that obtained by injecting only HA.…”

Section: Discussionmentioning

confidence: 99%

Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis

Yuan

Liu

et al. 2020

BioMed Research International

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Osteoarthritis (OA) is a joint disease characterized by cartilage degeneration. Osteopontin (OPN) is involved in the initiation, repair, and maintenance of metabolic homeostasis in normal articular cartilage. This study investigated the role of OPN and its interaction with the integrin ανβ3 receptor in the expression of hyaluronic acid (HA) in OA chondrocytes. Overexpression of OPN significantly increased the expression of integrin ανβ3 and hyaluronic acid synthases (HAS) and synthesis of HA. Depleting OPN in OA chondrocytes showed the opposite trend for integrin alpha;νβ3, HAS, and HA. Nonspecifically and specifically blocking integrin receptor using GRGDSP and integrin ανβ3 antibody downregulated HAS and HA; both were inhibited to similar extents. The expression of HAS and HA was predominantly regulated by the interaction between OPN and integrin ανβ3. Taken together, we have delineated the importance of the OPN/integrin ανβ3/HAS/HA axis in OA and identified OPN as a promising candidate for molecular therapy for use in patients with OA.

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“…In the article "Growth Factor Delivery to a Bovine Cartilage Defect Using Leukocyte-Rich Platelet-Rich Concentrates on a Hyaluronic Acid Scaffold," Titan, Schär, Hutchinson, Demange, Chen, and Rodeo 4 have performed a well-executed, high-quality in vitro investigation to assess whether human leukocyte-rich tissue platelet-rich plasma (L-PRP) or platelet-rich fibrin (L-PRF) delivered on a hyaluronic acid (HA) scaffold would improve tissue formation in a bovine chondral defect model. They found that both the HA scaffold combined with L-PRP and the HA scaffold combined with L-PRF showed greater biomechanical durability in vitro than the HA scaffold alone.…”

Section: See Related Article On Page 1431mentioning

confidence: 99%

“…As with many in vitro studies evaluating the effect of PRP on cartilage, 5 the translatability of the results of Titan et al 4 may be limited by several factors: the small size of the lesions, use of several human blood donors, lack of a biomechanical evaluation of axial compressive or horizontal shearing forces, and more. Nevertheless, this study is unique in terms of the rigorous methodology used, and its outcomes suggest that HA scaffolds augmented with L-PRP or L-PRF may play a role in Tel Aviv, Israel (R.G.)…”

Section: See Related Article On Page 1431mentioning

confidence: 99%

“…More high-quality, fundamental studies, such as the one published by Titan et al, 4 will provide a deeper understanding of the true merit of orthobiologic agents used in conjunction with cartilage restoration procedures. We envision that to achieve optimal outcomes, the formulation of the orthobiologic agents will need to be precisely tailored not just to a specific pathology but also to the individual patient.…”

Section: See Related Article On Page 1431mentioning

confidence: 99%

See 1 more Smart Citation

Editorial Commentary: Orthobiologics—The Evolution From Symptom to Structural Modification in the Treatment of Articular Cartilage Defects

Gilat¹,

Cole²

2020

Arthroscopy: The Journal of Arthroscopic & Related Surgery

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The race is on to identify the optimal recipe for the treatment of focal cartilage defects. Although many attempts had been made, beyond the use of osteochondral allografts, the restoration of pristine hyaline cartilage still seems distant and out of reach. The fundamental assumption is that cartilage restoration constructs must provide both structural integrity and adequate biological features that provide the optimal environment for hyaline cartilage formation. Augmentation of biologic scaffolds and grafts with orthobiologic agents may attend to both needs by improving defect fill and integration to the host articular cartilage interface.

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Growth Factor Delivery to a Cartilage-Cartilage Interface Using Platelet-Rich Concentrates on a Hyaluronic Acid Scaffold

Cited by 14 publications

References 44 publications

The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors

The Effects of Leukocyte-Platelet Rich Fibrin (L-PRF) on Suppression of the Expressions of the Pro-Inflammatory Cytokines, and Proliferation of Schwann Cell, and Neurotrophic Factors

Mechanistic Insight on the Interaction between OPN and Integrin ανβ3 in Osteoarthritis

Editorial Commentary: Orthobiologics—The Evolution From Symptom to Structural Modification in the Treatment of Articular Cartilage Defects

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