Growth-dependent effects of dietary protein concentration and quality on the biomechanical properties of the diaphyseal rat femur

Alippi, Rosa M.; Picasso, Emilio; Huygens, Patricia; Bozzini, Carlos E.; Bozzini, Clarisa

doi:10.1016/j.endonu.2011.09.005

Cited by 15 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Undernutrition (in response to either food restriction or exposure to hypoxia), which mainly affects protein and energy availability, affects bone biomechanical properties (21,29,(32)(33)(34)(35)(36) in growing rats by adapting the growth of the bone mass to that of the body mass. Bone mechanical quality of a whole bone (structural properties) depends on the integration of the 'mechanical quality of the mineralised tissue' (material stiffness mainly associated with collagen mineralisation) and the 'architectural quality of the structural bone design' (shape, size, architectural distribution of mineralised tissue) (37) .…”

Section: Discussionmentioning

confidence: 99%

Effect of chronic undernutrition on body mass and mechanical bone quality under normoxic and altitude hypoxic conditions

et al. 2016

Self Cite

View full text Add to dashboard Cite

Both undernutrition and hypoxia exert a negative influence on both growth pattern and bone mechanical properties in developing rats. The present study explored the effects of chronic food restriction on both variables in growing rats exposed to simulated high-altitude hypoxia. Male rats (n 80) aged 28 d were divided into normoxic (Nx) and hypoxic (Hx) groups. Hx rats were exposed to hypobaric air (380 mmHg) in decompression chambers. At T 0 , Nx and Hx rats were subdivided into four equal subgroups: normoxic control and hypoxic controls, and normoxic growth-restricted and hypoxic growth-restricted received 80 % of the amount of food consumed freely by their respective controls for a 4-week period. Half of these animals were studied at the end of this period (T 4 ). The remaining rats in each group continued under the same environmental conditions, but food was offered ad libitum to explore the type of catch-up growth during 8 weeks. Structural bone properties (strength and stiffness) were evaluated in the right femur midshaft by the mechanical three-point bending test; geometric properties (length, cross-sectional area, cortical mass, bending cross-sectional moment of inertia) and intrinsic properties of the bone tissue (elastic modulus) were measured or derived from appropriate equations. Bone mineralisation was assessed by ash measurement of the left femur. These data indicate that the growth-retarded effects of diminished food intake, induced either by food restriction or hypoxia-related inhibition of appetite, generated the formation of corresponding smaller bones in which subnormal structural and geometric properties were observed. However, they seemed to be appropriate to the body mass of the animals and suggest, therefore, that the bones were not osteopenic. When food restriction was imposed in Hx rats, the combined effects of both variables were additive, inducing a further reduction of bone mass and bone load-carrying capacity. In all cases, the mechanical properties of the mineralised tissue were unaffected. This and the capacity of the treated bones to undergone complete catch-up growth with full restoration of the biomechanical properties suggest that undernutrition, under either Nx or Hx conditions, does not affect bone behaviour because it remains appropriate to its mechanical functions.Key words: Undernutrition: Hypoxia: Bone quality: Biomechanics: Catch-up growth: Body massThe growing rat appears to follow an individual size-age trajectory to its body mass end point. This characteristic growth trajectory can be altered in rate and timing by exogenous modifiers. Two important non-genetic, environmental effects on the growth pattern are undernutrition (1)(2)(3)(4)(5)(6)(7)(8) and hypoxia (defined in terms of hypoxaemia, anaemia or increased Hb-oxygen affinity at sea level) (9)(10)(11)(12)(13) .On the basis of clinical paediatric findings (13)(14)(15) , we have developed a nutritional stress model in rats (nutritional growthretarded rats): weanling male rats placed on a 20 % restricted balan...

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of chronic undernutrition on body mass and mechanical bone quality under normoxic and altitude hypoxic conditions

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…For long bones, three‐point and four‐point bending tests are the most frequently used methods to evaluate extrinsic bone mechanical properties. Because of the weight‐loss or growth‐retarding effects of CR, PR, and MR, the correspondingly smaller bones generally have lower whole‐bone strength indices, such as the bending load (Nt), bending energy (mJ), and stiffness (Nt/mm) . This size‐related compromise is considered the primary negative effect of dietary restrictions on health, with increased risk for individuals with osteoporosis or osteopenia tendencies .…”

Section: Diet Restrictions and Bone Qualitymentioning

confidence: 99%

“…For growing young rats, the animals on a CR diet were smaller but had developed a bone strength that was sufficient for their size . Under ad libitum conditions, growing male rats that were fed MR diets or low‐quality protein were smaller in bone size, but they exhibited higher or unchanged material‐level strength in the femora . Van Wyhe et al .…”

Section: Diet Restrictions and Bone Qualitymentioning

confidence: 99%

“…Because of the weightloss or growth-retarding effects of CR, PR, and MR, the correspondingly smaller bones generally have lower whole-bone strength indices, such as the bending load (Nt), bending energy (mJ), and stiffness (Nt/mm). 23,31,33,139,140 This size-related compromise is considered the primary negative effect of dietary restrictions on health, with increased risk for individuals with osteoporosis or osteopenia tendencies. 20,31 However, according to Biewener's theory and previous studies, [64][65][66] body mass can induce a bias effect in dietary restriction studies.…”

Section: Viewpoints From Energy Metabolism Growth/aging and Bone Qumentioning

confidence: 99%

“…150,151 Under ad libitum conditions, growing male rats that were fed MR diets or low-quality protein were smaller in bone size, but they exhibited higher or unchanged material-level strength in the femora. 23,139,140 Van Wyhe et al fed commercial turkeys diets consisting of 80% and 60% calories and protein content, respectively. 143 Instead of conventionally comparing animals from different groups at the same age, they collected bone tissues of the control groups at 4, 8, 12, 16, and 20 weeks, then sampled the bone tissues of the 80% and 60% diet groups when the birds developed to body weights similar to those of the control birds at serial time points.…”

Section: Viewpoints From Energy Metabolism Growth/aging and Bone Qumentioning

confidence: 99%

See 2 more Smart Citations

Dietary restrictions, bone density, and bone quality

Huang

Ables

2016

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Caloric restriction (CR), protein restriction (PR), and specific amino acid restriction (e.g., methionine restriction (MR)) are different dietary interventions that have been confirmed with regard to their comprehensive benefits to metabolism and health. Based on bone densitometric measurements, weight loss induced by dietary restriction is known to be accompanied by reduced areal bone mineral density, bone mass, and/or bone size, and it is considered harmful to bone health. However, because of technological advancements in bone densitometric instruments (e.g., high-resolution X-ray tomography), dietary restrictions have been found to cause a reduction in bone mass/size rather than volumetric bone mineral density. Furthermore, when considering bone quality, bone health consists of diverse indices that cannot be fully represented by densitometric measurements alone. Indeed, there is evidence that moderate dietary restrictions do not impair intrinsic bone material properties, despite the reduction in whole-bone strength because of a smaller bone size. In the present review, we integrate research evidence from traditional densitometric measurements, metabolic status assays (e.g., energy metabolism, oxidative stresses, and inflammatory responses), and biomaterial analyses to provide revised conclusions regarding the effects of CR, PR, and MR on the skeleton.

show abstract

Biomechanical properties of the mandible, as assessed by bending test, in rats fed a low-quality protein

Bozzini

Champin

Alippi

et al. 2013

Archives of Oral Biology

View full text Add to dashboard Cite

Growth-dependent effects of dietary protein concentration and quality on the biomechanical properties of the diaphyseal rat femur

Cited by 15 publications

References 22 publications

Effect of chronic undernutrition on body mass and mechanical bone quality under normoxic and altitude hypoxic conditions

Effect of chronic undernutrition on body mass and mechanical bone quality under normoxic and altitude hypoxic conditions

Dietary restrictions, bone density, and bone quality

Biomechanical properties of the mandible, as assessed by bending test, in rats fed a low-quality protein

Contact Info

Product

Resources

About