Biomechanical properties of the mandible, as assessed by bending test, in rats fed a low-quality protein

Bozzini, Carlos E.; Champin, Graciela; Alippi, Rosa M.; Bozzini, Clarisa

doi:10.1016/j.archoralbio.2012.08.007

Cited by 11 publications

(16 citation statements)

References 27 publications

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“…Regarding development of our mechanical assay, rat mandibles are relatively small making fixture in mechanical testing devices a challenge. Typically, mandibles are tested in tension 33,34 or 3-point bending [35][36][37] configurations. We are interested in testing the mandibles in a bending mode since that is more similar to the in vivo biomechanical load.…”

Section: Discussionmentioning

confidence: 99%

Nanophase bone substitute for craniofacial load bearing application: Pilot study in the rodent

et al. 2017

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An exploratory pilot study shows that a rodent mandibular defect model is useful in determining the biological response to a nanophase collagen/apatite composite designed as a biomimetic load-bearing bone substitute. Using a critical size defect, eight groups of rats (n = 3) were implanted with four renditions of the nanophase bone substitute (NBS) biomaterial. Each rendition was tested with and without recombinant human bone morphogenetic protein 2 (BMP2). NBS biomaterial renditions were: baseline, hyper-densified, d-ribose crosslinked, and d-ribose crosslinked and hyper-densified. Biological outcomes were assessed surgically, radiologically, and histologically. With the limited power available due to the small N's involved, some interesting hypotheses were generated that will be more fully investigated in future studies. BMP2 loaded NBS, when uncrosslinked, resulted in robust bone formation in the entire defect volume (regardless of porosity). Unloaded NBS were well tolerated but did not cause significant new bone formation in the defect volume. Densification alone had little effect on in vivo performance. Crosslinking thwarted implant uptake of BMP2 and resulted in fibrous encapsulation. It is concluded that the nanophase bone substitute is well tolerated in this bone defect model. When loaded with BMP2, implantation resulted in complete bony healing and defect closure with implant density (porosity) having little effect on bone healing or remodeling. Without BMP2 the biomaterial did not result in defect closure. Crosslinking, necessary to increase mechanical properties in an aqueous environment, disrupts osteointegration and BMP2 uptake. Alternate implant fabrication strategies will be necessary to achieve an improved balance between material strength and osteointegration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 520-532, 2018.

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Section: Discussionmentioning

confidence: 99%

Nanophase bone substitute for craniofacial load bearing application: Pilot study in the rodent

et al. 2017

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“…For long bones, three‐point and four‐point bending tests are the most frequently used methods to evaluate extrinsic bone mechanical properties. Because of the weight‐loss or growth‐retarding effects of CR, PR, and MR, the correspondingly smaller bones generally have lower whole‐bone strength indices, such as the bending load (Nt), bending energy (mJ), and stiffness (Nt/mm) . This size‐related compromise is considered the primary negative effect of dietary restrictions on health, with increased risk for individuals with osteoporosis or osteopenia tendencies .…”

Section: Diet Restrictions and Bone Qualitymentioning

confidence: 99%

“…For growing young rats, the animals on a CR diet were smaller but had developed a bone strength that was sufficient for their size . Under ad libitum conditions, growing male rats that were fed MR diets or low‐quality protein were smaller in bone size, but they exhibited higher or unchanged material‐level strength in the femora . Van Wyhe et al .…”

Section: Diet Restrictions and Bone Qualitymentioning

confidence: 99%

“…Because of the weightloss or growth-retarding effects of CR, PR, and MR, the correspondingly smaller bones generally have lower whole-bone strength indices, such as the bending load (Nt), bending energy (mJ), and stiffness (Nt/mm). 23,31,33,139,140 This size-related compromise is considered the primary negative effect of dietary restrictions on health, with increased risk for individuals with osteoporosis or osteopenia tendencies. 20,31 However, according to Biewener's theory and previous studies, [64][65][66] body mass can induce a bias effect in dietary restriction studies.…”

Section: Viewpoints From Energy Metabolism Growth/aging and Bone Qumentioning

confidence: 99%

“…150,151 Under ad libitum conditions, growing male rats that were fed MR diets or low-quality protein were smaller in bone size, but they exhibited higher or unchanged material-level strength in the femora. 23,139,140 Van Wyhe et al fed commercial turkeys diets consisting of 80% and 60% calories and protein content, respectively. 143 Instead of conventionally comparing animals from different groups at the same age, they collected bone tissues of the control groups at 4, 8, 12, 16, and 20 weeks, then sampled the bone tissues of the 80% and 60% diet groups when the birds developed to body weights similar to those of the control birds at serial time points.…”

Section: Viewpoints From Energy Metabolism Growth/aging and Bone Qumentioning

confidence: 99%

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Dietary restrictions, bone density, and bone quality

Huang

Ables

2016

Annals of the New York Academy of Sciences

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Caloric restriction (CR), protein restriction (PR), and specific amino acid restriction (e.g., methionine restriction (MR)) are different dietary interventions that have been confirmed with regard to their comprehensive benefits to metabolism and health. Based on bone densitometric measurements, weight loss induced by dietary restriction is known to be accompanied by reduced areal bone mineral density, bone mass, and/or bone size, and it is considered harmful to bone health. However, because of technological advancements in bone densitometric instruments (e.g., high-resolution X-ray tomography), dietary restrictions have been found to cause a reduction in bone mass/size rather than volumetric bone mineral density. Furthermore, when considering bone quality, bone health consists of diverse indices that cannot be fully represented by densitometric measurements alone. Indeed, there is evidence that moderate dietary restrictions do not impair intrinsic bone material properties, despite the reduction in whole-bone strength because of a smaller bone size. In the present review, we integrate research evidence from traditional densitometric measurements, metabolic status assays (e.g., energy metabolism, oxidative stresses, and inflammatory responses), and biomaterial analyses to provide revised conclusions regarding the effects of CR, PR, and MR on the skeleton.

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Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring

Akyol

Çetin

Güleç

et al. 2018

J Dev Orig Health Dis

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Nutrition during pregnancy and lactation is a critical factor in the development of the offspring. Both protein content and source in maternal diet affect neonatal health, but the long-term effects of maternal low-quality protein diet on the offspring are less clear. This study aimed to examine the effects of maternal low-quality protein diet on offspring's growth, development, circulating metabolites and hepatic expression of methyltransferases. Virgin Wistar rats were mated at 11 weeks of age. Dams were then maintained on either a chow diet with 20% casein as the control group (C), or a low-quality protein diet with 20% wheat gluten as the experimental group (WG) throughout gestation and lactation. After weaning, all offspring were fed a control chow diet until the age of 20 weeks. Male WG offspring had significantly lower body weight and energy intake, whereas female WG offspring had significantly higher body weight and energy intake when compared with controls. Early life exposure to WG diet had no significant effect on circulating metabolites. However, fasting insulin concentrations and homoeostasis model assessment-insulin resistance were decreased in WG male and female offspring. Maternal low-quality protein diet increased plasma aspartic acid, glutamic acid, histidine, cystathione and decreased lysine in male WG offspring. Conversely, the same amino acids were reduced in female WG offspring. Adult offspring exposed to WG diet had significantly upregulated hepatic DNMT3a and DNMT3b expressions. Our study showed that there were differential effects of maternal poor-quality protein diet upon adult offspring's metabolism.

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Biomechanical properties of the mandible, as assessed by bending test, in rats fed a low-quality protein

Cited by 11 publications

References 27 publications

Nanophase bone substitute for craniofacial load bearing application: Pilot study in the rodent

Nanophase bone substitute for craniofacial load bearing application: Pilot study in the rodent

Dietary restrictions, bone density, and bone quality

Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring

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