Growth Curves in Muscle Nucleic Acid and Protein: Problems of Interpretation at the Level of the Muscle Cell

Burleigh, I. G.

doi:10.1016/b978-0-408-10638-2.50011-2

Cited by 12 publications

(12 citation statements)

References 84 publications

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“…Partly this could be explained by the number of nuclei per unit area, which also seemed to be higher in the children and lower among the postmenopausal women, respectively, as compared to the adult women. This is in accordance with the known principle of muscle growth characterized by the age-related increase in the size of the nuclear domain (Burleigh 1980). However, in the present study the numbers of subjects were too few for meaningful analysis, so further studies are needed.…”

Section: Er Expressionsupporting

confidence: 86%

Expression of both oestrogen receptor alpha and beta in human skeletal muscle tissue

Wiik

Ekman

Johansson

et al. 2008

Histochem Cell Biol

149

100

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There are two oestrogen receptors (ERs), ERalpha and ERbeta. ERbeta protein is expressed in human skeletal muscle in the nuclei of both myofibres and endothelial cells, whether ERalpha protein is present in this tissue is unknown. We studied the expression of ERalpha protein in human skeletal muscle biopsies taken from vastus lateralis from four men, four women, two children and two postmenopausal women. Immunohistochemistry was used to determine the proportions of nuclei that were positively stained for ERalpha, the proportion of ERalpha-positive nuclei located in the muscle fibres and in capillaries and to test for possible co-expression of ERalpha and ERbeta. Both ERs were expressed in all subjects. Of all nuclei, 63% stained for ERalpha with no sex difference. ERalpha was localised both in myofibres and in endothelial cells of the capillaries, 25% of the ERalpha-positive nuclei were located in the capillaries. ERalpha and ERbeta were generally expressed in the same nuclei. The present study shows for the first time the expression of ERalpha protein in human skeletal muscle independently of age and sex. These results might improve understanding of the physiological role of oestrogen in human skeletal muscle and raise new questions about activation of ERs in skeletal muscle.

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Section: Er Expressionsupporting

confidence: 86%

Expression of both oestrogen receptor alpha and beta in human skeletal muscle tissue

Wiik

Ekman

Johansson

et al. 2008

Histochem Cell Biol

149

100

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“…On a envisagé l'étude de l'action du mé-taprotérénol sur les protéines qui sont soumises constamment à des processus de synthèse et de dégradation, dans un équi-libre dynamique (Waterlow et al, 1978 (Burleigh, 1980). Par contre, la quantité d'ARN est représentative du processus de synthèse; plus précisément, la relation ARN/protéine est une mesure indirecte de la capacité de synthèse, c'est-à-dire de la quantité d'outils biosynthétiques, mais elle n'informe pas sur l'activité de synthèse (Waterlow et al, 1978).…”

Section: Introductionunclassified

“…Par contre, la quantité d'ARN est représentative du processus de synthèse; plus précisément, la relation ARN/protéine est une mesure indirecte de la capacité de synthèse, c'est-à-dire de la quantité d'outils biosynthétiques, mais elle n'informe pas sur l'activité de synthèse (Waterlow et al, 1978). Les (Burleigh, 1974(Burleigh, , 1980 (Obled et al, 1980). L'activité de cette enzyme était plus faible chez les animaux traités.…”

Section: Introductionunclassified

Modifications homéorhétiques produites par un β-agoniste dans les tissus musculaires et adipeux chez le rat

Portillo

Martínez²,

Larralde³

1991

Reprod. Nutr. Dev.

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Résumé ― Un agoniste p-adrénergique non sélectif, le métaprotérénol, administré par voie souscutanée (2 mg.kg-1 .j-1 ) pendant 23 j, induit une modification de la composition corporelle chez le rat. À cause de son effet «répartiteur», il produit une augmentation de la masse protéique de la carcasse (P < 0,05) et une diminution des dépôts adipeux (P < 0,01 ). Celle-ci s'accompagne d'un accroissement de la consommation d'oxygène dans le tissu adipeux scapulaire (P < 0,05) chez les animaux traités. Les résultats montrent que l'accroissement de la masse musculaire est dû à une diminution de la dégradation des protéines, mise en évidence par la diminution de l'activité de la cathepsine A plutôt qu'à un effet sur le processus de synthèse, mesuré par l'incorporation de phényla-lanine tritiée. Les résultats obtenus suggèrent une possible implication de la prostaglandine E 2 et de l'état rédox, comme médiateurs dans le mécanisme d'action des agonistes p-adrénergiques. métaprotérénol / homéorhesis / croissance / composition corporelle / métabolisme protéique Summary ― Homeorhetic changes produced by a p-agonist on adipose and muscle tissues In rat. A repartitioning effect was observed in young rats after a chronic treatment with a nonselective /3-agonist administered subcutaneously. An increase in carcass protein (P < 0.05) accompanied by a reduction in fat stores (P < 0.01) and an increase in back fat oxygen consumption (P < 0.05) were found in treated animals. The muscle mass accretion should be attributed to a reduction in muscle protein degradation, based on lower activity of the proteolytic enzyme cathepsin A in treated rats, rather than to changes in protein synthesis, assessed by an amino-acid incorporation technique. The

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“…Our measurements show that the growth-promoting effects on muscle are associated to a rise in the DNA content. Muscle DNA has been found to be increased in most circumstances in which sex or exogenous treatments enhanced muscle growth, which was inversely related to protein/DNA ratios (Burleigh 1980), although a recent report has indicated that the effect may be specific to certain fibre types (Maltin, Hay, Delday, Lobley and Reeds 1989).…”

Section: Discussionmentioning

confidence: 98%

Anabolic Actions of a Mixed β-Adrenergic Agonist on Nitrogen Retention and Protein Turnover

Ja¹,

Portillo²,

Larralde³

1991

Horm Metab Res

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Subcutaneous administration of a mixed beta-agonist induced increases in muscle (+13%) and heart (+17%) weights, which were accompanied by a reduction in back (-13%) and perirenal (-27%) fat stores in young male rats, while no changes in liver were observed. The values of nitrogen retention (mg/day) were significantly higher in the beta-agonist treated animals (+16%) as well as those of muscle DNA content (+8%). On the other hand, no statistically significant changes in muscle protein synthetic activity (g protein synthetized/g RNA) were detected (control: 13.4 vs treated: 14.6), while muscle proteolytic activity was decreased (-9%) in those rats administered with the repartitioning agent. In this context, it is suggested that the anabolic effect of metaproterenol should be attributed to a reduction in muscle protein degradation rather than to changes in protein synthesis.

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Growth Curves in Muscle Nucleic Acid and Protein: Problems of Interpretation at the Level of the Muscle Cell

Cited by 12 publications

References 84 publications

Expression of both oestrogen receptor alpha and beta in human skeletal muscle tissue

Expression of both oestrogen receptor alpha and beta in human skeletal muscle tissue

Modifications homéorhétiques produites par un β-agoniste dans les tissus musculaires et adipeux chez le rat

Anabolic Actions of a Mixed β-Adrenergic Agonist on Nitrogen Retention and Protein Turnover

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