Growth curve, biochemical profile and phytochemical analyses in calli obtained from the procambium segments of Bacupari

Abstract

Garcinia brasiliensis, popularly known as Bacupari, is native to the Amazon and commonly used in folk medicine Fukugetin,

Proteic and phenolics compounds contents in Bacupari callus cultured with glutamine and nitrogen sources

“…As for the other phenols, the proanthocyanidins are considered important antioxidants as they are effective reducing agents and more recently they have been investigated for having anti-tyrosinase activity. Recently, Santos-Filho et al (2014a) reported the presence of phenolic and flavonoid compounds and identified fukugetin, guttiferone-A and 7-epiclusianone in callus obtained from seeds of G. brasiliensis containing the procambium, though the occurrence of these compounds was less than in the initial explant. However, these authors mention that a higher content of secondary metabolites in the explant is common, since it still has its differentiated tissues, which favors the secondary metabolism (Santos-Filho et al, 2014a;Nicioli et al, 2010).…”

“…Next, they were washed three times in distilled and autoclaved water. The explants (2mm thickness and 4mm in diameter) were obtained from seed segments containing the procambium region as described by Santos-Filho et al (2014a). The explants were inoculated in culture flasks (30mm × 150mm, one explant per flask) containing 30mL of MS medium (Murashige and Skoog, 1962) solidified with 0.7% of agar and supplemented with sucrose (3%), BAP (6-Benzilaminopurine, 0.5 mg L -1 ) and different salt concentrations as follows: MS medium with original concentrations of nitrogen salts (20mM NH 4 NO 3 and 18.8mM KNO 3 ), MS medium with 10mM NH 4 NO 3 and 9.4mM KNO 3 known as ½ strength (MS½) and MS medium free of NH 4 NO 3 and KNO 3 containing glutamine in final concentrations of 5, 10, 30 and 60mM.…”

“…The supernatant was collected and stored at -20 °C (Lemos et al, 1999). Biochemical analyses were carried out on the 20 th , 80 th and 140 th days of cultivation following growth curve (Santos-Filho et al, 2014a). Total content of amino acids and proteins was determined according to Yemm et al (1955) and Bradford (1976), respectively.…”

“…Samples for phytochemical analysis were collected on the 140 th day after inoculation during the deceleration period of the culture, in accordance with the growth curve previously described by Santos-Filho et al (2014a). For the preparation of the extract, 100 mg of callus were macerated in 3 mL of ethanol.…”

“…Phytochemical analyses of this plant have resulted in the isolation of different biologically active substances, especially phenolic derivatives and polyprenylated benzophenones. Since G. brasiliensis callus have been established in vitro from explants obtained from seed segments containing the procambium region (Santos-Filho et al, 2014a). So, the aim of this study was to evaluate the influence of glutamine supplementation on the endogenous content of amino acids, proteins, total phenolic, flavonoids and proanthocyanidins in Bacupari callus.…”