Growth curve and development of the internal calli structure of Eucalyptus camaldulensis Dehn

Mendonça, Evânia Galvão; Paiva, Luciano Vilela; Stein, Vanessa Cristina; Pires, Marinês Ferreira; Santos, Breno Régis; Pereira, Fabricio José

doi:10.1590/s1516-89132012000600012

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…Interestingly, a subset of four unknown proteins was strongly up-regulated by CAG in calli; At1G47400 and AT1G47395, described both as hypothetical proteins, At2G30766; belongs to cytochrome P450 family and AT1G13609 which seems to be part of Defensin-like (DEFL) family protein. The last two genes have also been proved that they are up-regulated under Fe deficiency stress conditions but in leaves [ 36 ]. Fe is known as a component of the photosystems and essential for photosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis reveals responses to Cycloastragenol in Arabidopsis thaliana

et al. 2020

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Cycloastragenol (CAG), a molecule isolated from ‘Astragalus membranaceus’, stimulates the telomerase activity and cell proliferation significantly. It has been proven that CAG has the ability to prevent some diseases in humans. In this study, we aimed to figure out the CAG effects on the different signaling mechanisms in plants and to broadly analyze the genome-wide transcriptional responses in order to demonstrate CAG as a new key molecule that can potentially help plants to overcome different environmental stresses. RNA-seq strategy was employed to assess the transcriptional profiles in A. thaliana calli. Our work primarily focused on an overall study on the transcriptomic responses of A. thaliana to CAG. A total of 22593 unigenes have been detected, among which 1045 unigenes associated with 213 GO terms were differentially expressed and were assigned to 118 KEGG pathways. The up-regulated genes are principally involved in cellular and metabolic processes in addition to the response to a stimulus. The data analysis revealed genes associated with defense signaling pathways such as cytochrome P450s transporter, antioxidant system genes, and stress-responsive protein families were significantly upregulated. The obtained results can potentially help in better understanding biotic and/or abiotic tolerance mechanisms in response to CAG.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis reveals responses to Cycloastragenol in Arabidopsis thaliana

et al. 2020

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“…Meanwhile, at the stationary phase, no weight increase of culture occurs, but the maximum accumulation of secondary metabolites occurs. Cellular death happens during the decline phase [ 46 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Zaman

Azzeme

Ramle

et al. 2020

Plants

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Polyalthia bullata is an endangered medicinal plant species. Hence, establishment of P. bullata callus culture is hoped to assist in mass production of secondary metabolites. Leaf and midrib were explants for callus induction. Both of them were cultured on Murashige and Skoog (MS) and Woody Plant Medium (WPM) containing different types and concentrations of auxins (2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA), picloram, and dicamba). The callus produced was further multiplied on MS and WPM supplemented with different concentrations of 2,4-D, NAA, picloram, dicamba, indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA) media. The quantification of total phenolic content (TPC), total flavonoid content (TFC) and antioxidant capacity was further carried out on P. bullata callus, and the results were subjected to correlation analysis. Among the media, the WPM + 16.56 µM picloram (53.33 ± 22.06%) was the best for callus induction while MS + 30 µM dicamba was the best for callus multiplication. The TPC, TFC, and EC50 of DPPH scavenging activity were determined at 0.657 ± 0.07 mg GAE/g FW, 0.491 ± 0.03 mg QE/g, and 85.59 ± 6.09 µg/mL in P. bullata callus, respectively. The positive correlation between DPPH scavenging activity with TPC was determined at r = 0.869, and that of TFC was at r = 0.904. Hence, the P. bullata callus has an ability to accumulate antioxidants. It therefore can be a medium for secondary metabolites production.

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“…Growth curve revealed that specie displayed significant plasticity in growth rates across of the 30 days. The allocation of biomass to different plant organs depends on species, ontogeny, medium concentration and on the environment where of the explant growth (Mendonça et al, 2012;Chen, 2015). The effects of days were greater suggesting that just in the case of days, biomass allocation is an important factor in plant response to growth.…”

Section: Growth Curvementioning

confidence: 99%

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

Rocha

Araújo

Assis

et al. 2023

PCC&M

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Lippia dulcis Trevir. (Verbenaceae) is a species with medicinal potential that is used as a tranquilizer and to control diabetes in the western region of Pará, Brazil. The objective of this study was to determine the establishment and clonal propagation of Lippia dulcis through single nodal segments grown in different concentrations of salts in a MS (Murashige and Skoog) medium (MS, MS/2, MS/4) and determine the best period for subculturing the species. Nodal segments cultivated in the MS medium showed a higher survival percentage and better development than those grown in more dilute media (MS/2, MS/4). The concentration of the salts affected the dry weight gain of some plant parts. Plantlets cultivated in the MS medium obtained better leaf dry weight and total leaf area than plantlets cultivated in half-strength MS. The half-strength MS medium proved to be most effective for root length growth and root dry weight gainin vitro. The growth curve showed that day 30 was the time for subculturing the species. Starting with only one nodal segment, 3125 plantlets were obtained after sixth months of cultivation at a multiplication rate of five. The plantlets of Lippia dulcis were removed from the flasks and acclimatized with success in a greenhouse.

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Growth curve and development of the internal calli structure of Eucalyptus camaldulensis Dehn

Cited by 9 publications

References 21 publications

Transcriptomic analysis reveals responses to Cycloastragenol in Arabidopsis thaliana

Transcriptomic analysis reveals responses to Cycloastragenol in Arabidopsis thaliana

Induction, Multiplication, and Evaluation of Antioxidant Activity of Polyalthia bullata Callus, a Woody Medicinal Plant

Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures

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