Growth control of human foreskin fibroblasts and inhibition of extracellular sialidase activity by 2-deoxy-2,3-dehydro-N-acetylneuraminic acid.

Ganglioside Modulates Ligand Binding to the Epidermal Growth Factor Receptor

“…No clones showed increased activity at pH 4.0. To assure the validity of the assay further, a speci®c inhibitor of sialidase activity, 2,3-dehydro-2-deoxy-N-acetylneuraminic acid, was added to both control and sialidase-transfected cells (Usuki et al, 1988). The inhibitor reduced the level of sialidase activity at pH 6.8 in all cell lines to a basal level of 550±600 relative ¯uorescence units.…”

Metabolism and intracellular transport of glycosphingolipids

“…Glycosphingolipids, whose biological roles are gradually emerging, are thought to play a role in cell differentiation and morphogenesis (Hakomori, 1984a) and may conceivably contribute to the protection of the cell against environmental fluctuations. Though gangliosides have been identified as specific binding sites for viruses and toxins (Svennerholm, 1985) and have been implicated in the modulation of membrane-bound receptors (Bremer et al, 1986;Hanai et al, 1988) and in the control of the cell cycle (Usuki et al, 1988), the precise nature of their functional role and their seeming influence on overall membrane dynamics have yet to be defined.…”

Cluster Organization of Glycosphingolipid GD1a in Lipid Bilayer Membranes: A Dielectric and Conductometric Study

“…They are composed of hydrophilic carbohydrate chains linked to ceramide, which are hydrophobic moieties composed of a sphingoid base and a long-chain fatty acid. Gangliosides are ubiquitous constituents of cell membranes, where they show cell type-specific and differentiation expression patterns. , Functionally, gangliosides act as specific binding sites for bacterial toxins and viruses and are involved in membrane receptor modulation , and in the control of the cell cycle . Moreover, these molecules appear to be relevant in cell-cell interactions, antigen recognition, cell activation, and signal tranduction. − …”

“…2,3 Functionally, gangliosides act as specific binding sites for bacterial toxins 4 and viruses 5 and are involved in membrane receptor modulation 6,7 and in the control of the cell cycle. 8 Moreover, these molecules appear to be relevant in cell-cell interactions, antigen recognition, cell activation, and signal tranduction. [9][10][11] Although there is much information available on the chemistry of gangliosides, relatively little is known about their interactions with phospholipids and particularly about their organization and distribution either in synthetic or in biological membranes.…”