Growth characteristics ofEscherichia coliHB101[pGEc47] on defined medium

Abstract

This paper shows that differences in growth behavior of Escherichia coli strain HB101 and strain HB101[pGEc47] can be related to yeast extract‐enriched medium rather than plasmid properties. An optimal medium for growth of E. coli HB101[pGEc47] was designed based on the individual yield coefficients for specific medium components (NH4+ 6 g g−1, PO43− 14 g g−1, SO42− 50 g g−1). The yield coefficient for l‐leucine depends on the glucose content of the medium (20 g g−1 for 3% glucose, 40 g g−1 for 1% glucose) and the yield coefficient for l‐proline depends on the cultivation mode (20 g g−1 for batch cultivation, 44 g g−1 for continuous cultivation). Growth on defined medium after medium optimization is as rapid as on complex medium (0.42–0.45 h−1). The critical dilution rate (DR) in the defined medium above which undesired production of acetic acid occurs is in the range of 0.23–0.26 h−1. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:92–100, 1998.

Process development for production of recombinant human interferon-? expressed in Escherichia coli

“…The glucose concentration was easily maintained at zero throughout the fermentation, and acetate was controlled below 2 g l )1 . The final cell density was approximately 100 g dry cell wt l )1 after 36 h cultivation, which is equal to or higher than that reported by other researchers for recombinant plasmid-containing E. coli expressing foreign proteins [8,10,12,15,18,19,22,29,30,31,33]. Plasmid stability decreased continuously throughout the fermentation to $50% in the late fermentations, and the rHu-IFN-c concentration increased slightly to 3.4 g rHu-IFN-c l )1 (Fig.…”

“…M9 modified medium consisted of: 10 g glucose, 12.8 g Na 2 HPO 4 AE7H 2 O, 3 g KH 2 PO 4 , 0.5 g NaCl, 1 g NH 4 Cl, 0.24 g MgSO 4 ; 1 ml trace element solution per liter was used for preparation of seed culture, batch and fed-batch fermentations. [22].…”

Evaluation of software sensors for on-line estimation of culture conditions in an Escherichia coli cultivation expressing a recombinant protein

“…Since the medium used in the present study included constituents such as yeast extract, ammonium chloride, and sulfate, as well as a balance of CO 2 /HCO 3 , the initial deviation we obtained could to some extent be explained by this (cf. findings by Rothen et al, 1998). In principle, an extension of Eq.…”

An improved high cell density cultivation—iHCDC—strategy for leucine auxotrophic Escherichia coli K12 ER2507

“…Figure 2B displays a strong interactive effect of glucose and leucine on the yield coefficient y X/Leu . Rothen et al [4] report for E. coli HB101, which is auxotrophic for thiamine, proline, and leucine, a similar Table 1. Factor settings and results for the full factorial DoE approach to identify maximum specific growth rate and biomass yield coefficient based on leucine reduction in growth rate and yield coefficient.…”