Growth Characteristics of Chlorella sorokiniana in a Photobioreactor during the Utilization of Different Forms of Nitrogen at Various Temperatures

Abstract

The cultivation of microalgae requires the selection of optimal parameters. In this work, the effect of various forms of nitrogen on the growth and productivity of Chlorella sorokiniana AM-02 when cultivated at different temperatures was evaluated. Regardless of the temperature conditions, the highest specific growth rate of 1.26 day−1 was observed in modified Bold’s basal medium (BBM) with NH4+ as a nitrogen source, while the highest specific growth rate in BBM with NO3− as a nitrogen source achieved only 1.07 day−1. Moreover, C. sorokiniana grew well in medium based on anaerobic digester effluent (ADE; after anaerobic digestion of chicken/cow manure) with the highest growth rate being 0.92 day−1. The accumulation of proteins in algal cells was comparable in all experiments and reached a maximum of 42% of dry weight. The biomass productivity reached 0.41–0.50 g L−1 day−1 when cultivated in BBM, whereas biomass productivity of 0.32–0.35 g L−1 day−1 was obtained in ADE-based medium. The results, based on a bacterial 16S rRNA gene sequencing approach, revealed the growth of various bacterial species in ADE-based medium in the presence of algal cells (their abundance varied depending on the temperature regimen). The results indicate that biomass from C. sorokiniana AM-02 may be sustainable for animal feed production considering the high protein yields.

Anaerobic Digestion of Chicken Manure in the Presence of Magnetite, Granular Activated Carbon, and Biochar: Operation of Anaerobic Reactors and Microbial Community Structure

“…Thus, biomethane released from anaerobic reactors can be further used for the local production of heat and electricity [ 4 ]. In addition, CO 2 from biogas and nutrients from digestate can be used to produce algal biomass [ 5 , 6 , 7 ].…”

Application of chlorella vulgaris cultures in biotechnology and food industry

“…Изготовители продуктов питания, кормов, косметики, товаров для здоровья, также заинтересованы в повышении продуктивности C. Vulgaris [1]. Промышленные предприятия, культивирующие C. vulgaris в крупных масштабах, испытывают потребность в эффективных способах усиления роста и продуктивности C. Vulgaris [14,47,49].…”

Biotechnological Approaches for Biomass and Lipid Production Using Microalgae Chlorella and Its Future Perspectives

“…Chlorella can use ammonium for growth, making it possible to use this alga for bioremediation to remove ammonium [75]. Ziganshina et al reported that the highest growth rate (1.26/day) was observed in modified Bold's basal medium (BBM) with ammonium, while the growth rate in BBM with nitrate was only 1.07/day [76]. Thus, although Chlorella strains seem tolerant to ammonium, the degree of growth inhibition by ammonium varies between the strains [71,77,78].…”