Biotechnological Approaches for Biomass and Lipid Production Using Microalgae Chlorella and Its Future Perspectives

Abstract

Heavy reliance on fossil fuels has been associated with increased climate disasters. As an alternative, microalgae have been proposed as an effective agent for biomass production. Several advantages of microalgae include faster growth, usage of non-arable land, recovery of nutrients from wastewater, efficient CO 2 capture, and high amount of biomolecules that are valuable for humans. Microalgae Chlorella spp. are a large group of eukaryotic, photosynthetic, unicellular microorganisms with high adaptability to environmental variations. Over the past decades, Chlorella has been used for the large-scale production of biomass. In addition, Chlorella has been actively used in various food industries for improving human health because of its antioxidant, antidiabetic, and immunomodulatory functions. However, the major restrictions in microalgal biofuel technology are the cost-consuming cultivation, processing, and lipid extraction processes. Therefore, various trials have been performed to enhance the biomass productivity and the lipid contents of Chlorella cells. This study provides a comprehensive review of lipid enhancement strategies mainly published in the last five years and aimed at regulating carbon sources, nutrients, stresses, and expression of exogenous genes to improve biomass production and lipid synthesis.

Ecotoxicity of o-Chlorobenzylidene Malononitrile (CBM) and Toxicological Risk Assessment for SCLP Biological Cultures (Saccharomyces sp., Chlorella sp., Lactobacillus sp., Paramecium sp.)

“…was cultivated in Erlenmeyer flasks containing 250 cm 3 of distilled water, MgSO 4 ·7H 2 O (0.3 g), KNO 3 (0.4 g), CaCl 2 (0.4 g), NaH 2 PO 4 ·2H 2 O (0.3 g), FeSO 4 ·7H 2 O (0.02 g), NaNO 3 (0.3 g), and NH 4 Cl (0.2 g); pH was adjusted to 6.5. Erlenmeyer flasks were placed in a laboratory shaker (ORBITAL Multi-Shaker) at 100 rpm for optimal aeration at 35 °C ± 1 °C [ 21 , 22 ].…”