Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities

Abstract

Abstract. Indrayani I, Haslianti H, Asmariani A, Muskita WH, Balubi M. 2020. Growth, biomass and lipid productivity of a newly isolated tropical marine diatom, Skeletonema sp. UHO29, under different light intensities. Biodiversitas 21: 1498-1503. Light is one of the important factors affecting growth and biochemical composition of microalgae. The aim of this study was to determine growth, biomass and lipid productivity of a newly isolated marine diatom, Skeletonema sp.UHO29 under different light intensities. The Skeletonema sp. was cultured in 300 mL conical flasks containing 150 mL of f/2 medium under three different light intensities (28, 60 and 110 μmol photons m-2 s-1 ) with three replications. The cultures were incubated under ambient room temperatures, and a cycle of 12 hours light and 12 hours dark, in batch modes for two weeks. The results showed that the highest specific growth rate (0.56 d-1) was achieved at the highest light intensity (110 μmol photons m-2 s-1) and the lowest (0.11 d-1) was obtained at the lowest light intensity (28 μmol photons m-2 s-1). The highest biomass yield (0.62 g L-1) and biomass productivity (0.34 g L-1 d-1) was achieved at the highest light intensity. However, the highest lipid yield (0.175 g L-1), lipid content (28.78% ash-free dry weight) and lipid productivity (0.067 g L-1 d-1) were achieved at medium light intensity. The alga prefers high light intensity for higher growth rate and biomass productivity but it produces more lipids at medium light intensity. This study indicated that the Skeletonema sp.UHO29 is a potential species for outdoor mass cultivation and is a promising microalgal strain for use as a biodiesel feedstock due to its high growth rate, high biomass and lipid productivity.

Growth, biomass, and carotenoids content analysis of Navicula sp. and Chlorella sp. in batch cultures with different salinities

“…The filtered seawater is then kept in a 1000 mL Erlenmeyer and wrapped in aluminum foil for the following sterilization process using an autoclave (Wiseclave WACS-1045). The seawater sterilization was done at 121°C for 15 minutes to ensure the absence of contamination by biotic pathogens (Indrayani et al 2018(Indrayani et al , 2019(Indrayani et al , 2021. The seawater filtering and preparation were carried out at the Productivity and Aquatic Environment Laboratory, Faculty of Fisheries and Marine Sciences, Halu Oleo University, Kendari, Indonesia.…”

“…The immense potential of microalgae found rooted in diverse strains makes the seawaters of Southeast Sulawesi essential to be studied and explored spatially. The information that may be further explored can range from species according to their geography, growth promoter (Solimeno et al 2015;Sathasivam et al 2018;Zhuang et al 2021) and contents for optimal utilization as prime raw material in the nutraceutical industry (Indrayani et al 2018(Indrayani et al , 2019(Indrayani et al , 2021Hynstova et al 2018;Helena et al 2020;Sathasivam et al 2018;Zhuang et al 2021).…”

“…Several environmental parameters, especially salinity, strongly influence the physiology of marine microalgae. Fluctuations in water salinity, or 'anomalies', directly affect microalgae biological activity (El-Baz et al 2002;Indrayani et al 2018Indrayani et al , 2019Indrayani et al , 2021Pisal and Lele 2005). This observation of the salinity-induced activity phenomenon is the scientific basis for the different salinity treatments introduced to microalgae cultivation media obtained from Southeast Sulawesi waters.…”

“…in batch cultures with different salinities" is essential to do, considering the limited availability of the latest related research, particularly in Southeast Sulawesi waters. Until this article was written, the current research was only conducted by Indrayani et al (2021). Hence, this study aims to determine the effect of different salinities on cultivation media on the growth, biomass, and carotenoids content of Chlorella sp.…”