The present study was conducted at Research farm of R.A.K., college of Agriculture, Sehore, Madhya Pradesh during 2020-21. The experiment was laid out using Randomized Block Design with different levels of Phosphorus with or without PSB application (T1- 45 kg P2O5/ha, T2- 60 kg P2O5/ha, T3- 75 kg P2O5/ha, T4-90 kg P2O5/ha, T5 - 45 kg P2O5/ha + PSB @ 5g/kg seed, T6 - 60 kg P2O5/ha + PSB @ 5g/kg seed, T7 - 75 kg P2O5/ha + PSB @ 5g/kg seed, T8 - 90 kg P2O5/ha + PSB @ 5g/kg seed). Treatment T7 found to be best for yield and yield attributes of berseem. Maximum plant height (54.8, 71.5 and 65.5 cm), number of branches/plant (5.44, 6.72 and 8.53), dry matter accumulation (4.90, 6.64 and 5.00 g/plant) at 1st cutting, 2ndcutting and at harvest respectively, dry matter (13.01 %), crude protein (19.99 %), crude protein yield (420.21 kg/ha), Gross return (87,660 ₹/ha), net return (59,412 ₹/ha) and B:C ratio (3.03). While minimum recorded with treatment T1 (45kg P2O5/ha without PSB application).