Growth and Development of Strongyloides ratti Sandground, 1925, in the Albino Rat

Wertheim, Guta; Lengy, J.

doi:10.2307/3276249

Cited by 35 publications

(9 citation statements)

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“…There was an equal distribution of worms between the anterior and posterior segments on day 6 and 60% of the worms were found in the last quarter of the intestine on day 8. DISCUSSION Previous work has shown that S. ratti arrives in the small intestine from about 24 hours after infection (Spindler, 1958;Abadie, 1963;Wertheim and Lengy, 1965). Whilst we did not attempt to determine the first time that they could be found in the intestine we did find (for example Table 2) that the number of adult worms increased until about 9 days after infection.…”

Section: Methodsmentioning

confidence: 77%

“…They reach the lungs a few hours later where they grow but do not moult. The first larvae migrate to the intestinal tract within 24 hours of infection (Spindler, 1958;Abadie, 1963;Wertheim and Lengy, 1965) where they moult twice and start to produce eggs about 4 days after infection. During the first few days after their arrival the majority of adult worms occupy the anterior third of the small intestine (Abadie, 1963;Wertheim, 1970).…”

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confidence: 99%

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Strongyloides ratti: 1. Parasitological observations on primary and secondary infections in the small intestine of rats

Moqbel

Denham

1977

J. Helminthol.

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Section: Methodsmentioning

confidence: 77%

mentioning

confidence: 99%

Strongyloides ratti: 1. Parasitological observations on primary and secondary infections in the small intestine of rats

Moqbel

Denham

1977

J. Helminthol.

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“…The Trichuris muris mouse model is an excellent model for trichuriasis [19], [20]. Strongyloides ratti in rats is a commonly used murine model for strongyloidiasis [21]. Finally, murine infection with Ascaris suum is a model which mimics the early infection of A. lumbricoides .…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Efficacy of Monepantel (AAD 1566) against Laboratory Models of Human Intestinal Nematode Infections

2011

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BackgroundFew effective drugs are available for soil-transmitted helminthiases and drug resistance is of concern. In the present work, we tested the efficacy of the veterinary drug monepantel, a potential drug development candidate compared to standard drugs in vitro and in parasite-rodent models of relevance to human soil-transmitted helminthiases.MethodologyA motility assay was used to assess the efficacy of monepantel, albendazole, levamisole, and pyrantel pamoate in vitro on third-stage larvae (L3) and adult worms of Ancylostoma ceylanicum, Necator americanus and Trichuris muris. Ancylostoma ceylanicum- or N. americanus-infected hamsters, T. muris- or Ascaris suum-infected mice, and Strongyloides ratti-infected rats were treated with single oral doses of monepantel or with one of the reference drugs.Principal FindingsMonepantel showed excellent activity on A. ceylanicum adults (IC50 = 1.7 µg/ml), a moderate effect on T. muris L3 (IC50 = 78.7 µg/ml), whereas no effect was observed on A. ceylanicum L3, T. muris adults, and both stages of N. americanus. Of the standard drugs, levamisole showed the highest potency in vitro (IC50 = 1.6 and 33.1 µg/ml on A. ceylanicum and T. muris L3, respectively). Complete elimination of worms was observed with monepantel (10 mg/kg) and albendazole (2.5 mg/kg) in A. ceylanicum-infected hamsters. In the N. americanus hamster model single 10 mg/kg oral doses of monepantel and albendazole resulted in worm burden reductions of 58.3% and 100%, respectively. Trichuris muris, S. ratti and A. suum were not affected by treatment with monepantel in vivo (following doses of 600 mg/kg, 32 mg/kg and 600 mg/kg, respectively). In contrast, worm burden reductions of 95.9% and 76.6% were observed following treatment of T. muris- and A. suum infected mice with levamisole (200 mg/kg) and albendazole (600 mg/kg), respectively.Conclusions/SignificanceMonepantel reveals low or no activities against N. americanus, T. muris, S. ratti and A. suum in vivo, hence does not qualify as drug development candidate for human soil-transmitted helminthiases.

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“…ratti is a parasitic nematode commonly used as a model of nematode infection in experimental animals (Sheldon 1937;Spindler 1958;Wertheim and Lengy 1965). The larvae usually live in soil and infect their rat host percutaneously, where they migrate to the head and finally reach the small intestine (Tada et al 1979;Dawkins 1989;Koga et al 1998Koga et al , 1999.…”

Section: Introductionmentioning

confidence: 98%

Strongyloides ratti: thermokinesis of glycolytic enzyme- and lectin-treated third-stage infective larvae in vitro

Tobata-Kudo¹,

Kudo

Tada

2005

Parasitol Res

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The infective third-stage larvae (L3s) of a parasitic nematode of rodents, Strongyloides ratti, showed three types of thermokinesis on a temperature gradient using an in vitro agarose tracking assay method. These depended both on the pattern of gradient temperature and the prior culture temperature. Most L3s (> or = 80%) isolated from rat feces cultured at 25 degrees C and placed on a gradient at temperatures between 30 degrees C and 37 degrees C showed no directional response, at 22-29 degrees C more than 50% of the L3s showed positive thermokinesis, at 21 degrees C L3s showed positive, negative and no directional responses in the same ratio, while at 18-20 degrees C, L3s showed negative thermokinesis (approx. 40%) or no directional response (approx. 60%) as in our previous study. The present study describes the effects of glycolytic enzyme- and lectin-treated positive thermokinesis of L3s. alpha-Glucosidase or concanavalin A significantly exhibited inhibitory effects on thermokinesis.

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Growth and Development of Strongyloides ratti Sandground, 1925, in the Albino Rat

Cited by 35 publications

References 5 publications

Strongyloides ratti: 1. Parasitological observations on primary and secondary infections in the small intestine of rats

Strongyloides ratti: 1. Parasitological observations on primary and secondary infections in the small intestine of rats

In Vitro and In Vivo Efficacy of Monepantel (AAD 1566) against Laboratory Models of Human Intestinal Nematode Infections

Strongyloides ratti: thermokinesis of glycolytic enzyme- and lectin-treated third-stage infective larvae in vitro

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