Abstract. The present study investigated the supplementation of soursop leaves powder (Annona muricata L.) on body weight gain and carcass percentage of male Tegal duck. The study was conducted from 29 November 2015 to 3 January 2016 in duck cage in Sokaraja Kulon, Purwokerto. One hundred male Tegal duck were fed basal feed consisted of 30% corn, 7% soy bean meal, 6.1% vegetable oil, 17% poultry meat meal, 38.2% ricebran, 0.1% L-lysin HCL, 0.3% DL-methionin, 0.2% topmix, 0.1% NaCl, and 1% CaCO3. The experimental research used completely randomized design involving basal feed plus 0, 5, 10, and 15% supplemental soursop leaves meal, each with 5 replicates. The diameter of muscle fiber, lipid cell, body weight gain and carcass percentage were measured. The obtained data were subject to analysis of variance followed by orthogonal polynomial test. Result showed that treatments non significantly (P>0.05) affected the diameter of chest muscle fiber, carcass percentage and carcass but significantly affected (P<0.05) body weight gain with equation Y = 427.74 -67.10 X + 2.27 X 2 . Conclusively, supplemental soursop leaves meal (Annona muricata L.) in feed cannot increase the muscle fiber diameter of intermuscular lipid cell, carcass percentage and carcass components. Excessive supplement even lowers body weight gain of male Tegal duck. Key words: soursop leaves, flavonoid, carcass, muscle fiber, male Tegal duck Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi tepung daun sirsak (Annona muricata L.) dalam pakan terhadap pertambahan bobot badan dan persentase karkas pada itik Tegal jantan. Penelitian telah dilaksanakan pada 29 November 2015 -3 Januari 2016 di kandang itik Sokaraja Kulon, Purwokerto. Materi penelitian menggunakan 100 ekor itik tegal jantan, pakan basal yang diberikan terdiri atas jagung 30%, tepung kedelai 7%, minyak sayur 6,1%, tepung daging 17%, dedak 38,2%, L-lysin HCL 0,1%, DLmethionin 0,3%, topmix 0,2%, NaCl 0,1%, dan CaCO3 1%. Metode penelian yang digunakan metode eksperimen dengan rancangan Rancangan Acak Lengkap. Perlakuan terdiri atas : pakan basal ditambah 0% tepung daun sirsak, pakan basal ditambah 5% tepung daun sirsak, pakan basal ditambah 10% tepung daun sirsak, pakan basal ditambah 15% tepung daun sirsak. Tiap perlakuan diulang 5 kali dan variabel yang diamati adalah diameter otot, sel lemak, pertambahan bobot badan dan carcass percentage. Data yang diperoleh dianalisis menggunakan analisis ragam dan dilanjutkan dengan uji orthogonal polinomial. Hasil analisis ragam menunjukkan bahwa perlakuan berpengaruh tidak nyata (P>0,05) terhadap diameter of breast muscle fiber, tebal sel lemak, carcass percentage dan bagian-bagian karkas, akan tetapi berpengaruh nyata (P<0,05) terhadap pertambahan bobot badan dengan persamaan garis Y = 427,74 -67,10X + 2,27X 2 . Kesimpulan penelitian ini adalah penambahan tepung daun sirsak (Annona muricata L.) dalam pakan belum mampu meningkatkan diameter otot, sel lemak intramuskuler dan persentase karkas dan bagian-bagiannya, bahkan jika pember...