Growth and Behavior of Cell Cultures: Embryogenesis and Organogenesis

Evans, David A.; Sharp, W. R.; Flick, Christopher E.

doi:10.1016/b978-0-12-690680-6.50008-5

Cited by 148 publications

(55 citation statements)

References 198 publications

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“…This difference with the results presented here might be explained by the use, in the present study, of 2,4-D in the induction medium. This auxin is known to have a positive influence on embryogenic (and rhizogenic) callogenesis (Evans et al ., 1981 ;Litz, 1984;Desai et al ., 1985 ;Rapela, 1985) . Furthermore, most authors reporting somatic embryogenesis in rose also used 2,4-D (Noriega & Sondhal,1991;Rout et al ., 1988) .…”

Section: Discussionmentioning

confidence: 99%

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

Aréne¹,

Pellegrino²,

Gudin³

1993

Euphytica

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Flowering plants of Rosa hybrida L . cv Meirutral have been obtained either from direct regeneration of adventitious shoots on leaf and root fragments, or through organogenesis and somatic embryogenesis on calli derived from anther, ovule, petal, sepal, receptacle, leaf, stem internode, root and zygotic embryo tissues . The calli derived from floral parts exhibited rhizogenesis . In this case direct induction of adventitious shoots from selected roots had to be performed in order to generate plants . A histological study of the morphogenetic calli was carried out . The plants regenerated directly and those regenerated from calli of leaf, stem internode, root and zygotic embryo tissues, together with reference plants propagated by cuttings, were compared on a phenotypic basis by taking into account petal number, form and colour, and plant growth habit . From these observations, it can be concluded that directly regenerated plants are as stable as reference plants while plants regenerated from callus are unstable, especially those derived from zygotic embryo tissues .

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Section: Discussionmentioning

confidence: 99%

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

Aréne¹,

Pellegrino²,

Gudin³

1993

Euphytica

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“…Infact, of the four species studied in relation to this problem and based on the small number of accessions, only two species (A. caudatus and A. hypochondriacus) responded positively and, in particular, only one variety for each of these species showed the capacity to regenerate in good frequency plantlets from callus. Moreover, it is interesting to note that some varieties (and species), although cultured in presence of a high level of cytokinin which is a causative agent in bud differentiation in vitro (Skoog & Miller, 1957;Evans et al, 1981;Profumo et al, 1985), regenerate roots.…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of species and varieties of four Amaranthus L. species

1992

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Callus induction, callus growth and organogenetic processes were investigated in hypocotyl and stem cultures of four species of Amaranthus each of which comprised several varieties. Callus formation occurred in almost all the varieties in 100% of explants. The combinations of naphtaleneacetic acid plus benzylaminopurine or 2,4-dichlorophenoxyacetic acid plus Kinetin were very effective in causing callus formation; the results for callus growth in presence of the same growth regulator combinations revealed in some cases significant differences among the varieties.As far organogenesis-based on a few varieties-only A. caudatus and A. hypochondriacus responded well forming shoots from callus when cultured in presence of indoleacetic acid plus Kinetin and/or indoleacetic acid plus benzylaminopurine. Root regeneration was also observed in several varieties. Complete plantlets were obtained from rooted shoots.The results are discussed in relation to hormonal effect and the genotype importance.

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“…The problem of inducing and maintaining callus in cereal cultures has been resolved to an extent by the use of 2,4-dichlorophenoxyacetic acid (2,4-D) (Evans et al ., 1981) . While 2,4-D is necessary for effective callus initiation and production, cytological and genetical abnormalities are known to result (Sunderland, 1977 ;Deambrogio & Dale, 1980) .…”

Section: Introductionmentioning

confidence: 99%

Long-term callus cultures of diploid Barley (Hordeum vulgare). I. Auxin effects on culture initiation and maintenance

Ziauddin

Kasha

1990

Euphytica

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Use of 2,4-D was superior to NAA or IAA for embryogenic callus initiation or maintenance in barley cultivar Bruce . A concentration of at least 2.0 mg/l 2,4-D was desirable for culture initiation . The developmental size of the embryo was more important than embryo age for obtaining embryogenic calli . Even brief exposures (20-40 days) of calli to concentrations of higher than 5 .0 mg/l 2,4-D or 10 .0 mg/l NAA resulted in inhibition of subsequent plant regeneration and therefore, concentrations above these could not be used for maintenance cultures . In the long-term maintenance cultures, the best production of embryogenic calli was with 0 .

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Growth and Behavior of Cell Cultures: Embryogenesis and Organogenesis

Cited by 148 publications

References 198 publications

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

A comparison of the somaclonal variation level of Rosa hybrida L. cv Meirutral plants regenerated from callus or direct induction from different vegetative and embryonic tissues

In vitro culture of species and varieties of four Amaranthus L. species

Long-term callus cultures of diploid Barley (Hordeum vulgare). I. Auxin effects on culture initiation and maintenance

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