In vitro culture of species and varieties of four Amaranthus L. species

Bennici, A.; Schiff, Silvia; Bovelli, Ruggero

doi:10.1007/bf00041752

Cited by 20 publications

(6 citation statements)

References 12 publications

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“…When a high concentration of auxin (2,4-D) was used in combination with lower concentration of cytokinin (BAP), a low frequency of callus induction was noted (Table 2). Similar reports of 2, 4-D plus kinetin or NAA plus BAP on callus development was observed in Amaranthus by Flores et al (1982) and Bennici et al (1992). When a high concentration of cytokinin was used with a low concentration of auxin, a low frequency of callus induction was noted.…”

Section: Callus Inductionsupporting

confidence: 82%

“…In previous research, Bennici et al (1992) studied 4 different species of Amaranthus (A. caudatus, A. hypochondriacus, A. cruentus and A. hybridus) for callus induction, growth and organogenic processes from hypocotyl and epicotyl cultures and found that 5.4 µM of naphthalene acetic Acid (NAA) plus 4.4 µM of benzyl amino purine (BAP) or 2.3 µM of 2,4-dichlorophenoxyacetic acid (2,4-D) plus 2.3 µM of kinetin were the best combinations for callus induction and complete plantlets were obtained from rooted adventitious shoots. Callus production and shoot regeneration from primary callus or hypocotyl explants have been reported by Flores et al (1982), Flores and Teutonico (1986) in A. cruentus, A. hypochondriacus, A. tricolor and by Bagga et al (1987) in A. paniculatus.…”

Section: Introductionmentioning

confidence: 87%

“…Some rooting response was also observed on MS medium supplemented with 1 mg/L NAA. Bagga et al (1987) found that hypocotyl segments of A. paniculatus formed roots on B5 medium supplemented with NAA and Bennici et al (1992) reported that amaranthus responded well in forming roots with IAA plus kinetin and/or IAA plus BAP.…”

Section: Rooting Of Regenerated Shootsmentioning

confidence: 99%

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In vitro propagation of Alternanthera sessilis (sessile joyweed), a famine food plant

Singh¹,

Kandasamy²,

Odhav

2009

Afr. J. Biotechnol.

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A complete protocol for micropropagation of Alternanthera sessilis using leaf explants was developed. Leaf explants from A. sessilis established on Murashige and Skoog medium were treated with various concentrations and combinations of auxins and cytokinins to determine the best method for callus formation, shoot regeneration and root formation. MS medium supplemented with 1 mg/l BAP and 1 mg/l 2,4-D was determined to be the most suitable for callus induction. After 2 weeks, the callus was then transferred to shooting media which consisted of half strength MS basal medium, 1 mg/l IAA and 1 mg/ l BAP. After 4 weeks, structures were observed with their basal ends embedded on the callus. These structures turned into green colored shoot buds. MS medium supplemented with 1 mg/l IAA and 1 mg/l BAP showed maximum shoot multiplication. For rooting, the optimal medium was half strength MS medium supplemented with 1 mg/l IBA. Rooted plantlets were then transferred to sunbag vessels which provided the high humidity environment. The hardened plants were then successfully established in the soil medium and can function in the natural environment.

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Section: Callus Inductionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 87%

See 1 more Smart Citation

In vitro propagation of Alternanthera sessilis (sessile joyweed), a famine food plant

Singh¹,

Kandasamy²,

Odhav

2009

Afr. J. Biotechnol.

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“…High molecular weight DNA that was also of high quality and purity was extracted for the GBS method using tissue from aseptically grown seedlings. To obtain fresh tissue for the extraction, seeds were sterilized in 5% HgCl 2 for 5 min followed by 5 min rinses in autoclaved water ( Bennici et al, 1992 ). Sterilized seed was then transferred into individual magenta box (Sigma–Alrich Co. LLC), one per genotype containing 100 ml of Murashige-Skoog media with 2 g of sucrose and placed in a growth chamber for 14 days.…”

Section: Methodsmentioning

confidence: 99%

Diversity in Grain Amaranths and Relatives Distinguished by Genotyping by Sequencing (GBS)

Blair

2017

Front. Plant Sci.

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The genotyping by sequencing (GBS) method has become a molecular marker technology of choice for many crop plants because of its simultaneous discovery and evaluation of a large number of single nucleotide polymorphisms (SNPs) and utility for germplasm characterization. Genome representation and complexity reduction are the basis for GBS fingerprinting and can vary by species based on genome size and other sequence characteristics. Grain amaranths are a set of three species that were domesticated in the New World to be high protein, pseudo-cereal grain crops. The goal of this research was to employ the GBS technique for diversity evaluation in grain amaranth accessions and close relatives from six Amaranthus species and determine genetic differences and similarities between groupings. A total of 10,668 SNPs were discovered in 94 amaranth accessions with ApeKI complexity reduction and 10X genome coverage Illumina sequencing. The majority of the SNPs were species specific with 4,568 and 3,082 for the two grain amaranths originating in Central America Amaranthus cruentus and A. hypochondriacus and 3,284 found amongst both A. caudatus, originally domesticated in South America, and its close relative, A. quitensis. The distance matrix based on shared alleles provided information on the close relationships of the two cultivated Central American species with each other and of the wild and cultivated South American species with each other, as distinguished from the outgroup with two wild species, A. powellii and A. retroflexus. The GBS data also distinguished admixture between each pair of species and the geographical origins and seed colors of the accessions. The SNPs we discovered here can be used for marker development for future amaranth study.

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“…At the same time as the main objective of obtaining regenerants, researchers obtained a callus tisssue. Rapid and intensive callus formation from hypocotyl explants was observed for A. cruentus L. (6 mg/l NAA + 0.1 mg/l BA) and A. hybridus L. (6 mg/l 2.4-D + 0.1 mg/l KIN (Bennici et al, 1992). Arya and colleagues chose A. paniculatus L. as an object of research.…”

Section: Achievements In Regeneration Of Amaranthus L Speciesmentioning

confidence: 99%

Achievements in Genetic Engineering of Amaranthus L. Representatives

Yaroshko¹

2021

International Journal of Secondary Metabolite

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Despite the fact that in the modern world more than a thousand edible plants are used for food, only 3 staple cereal crops are grown worldwide: wheat, rice, and maize. Growing a limited number of crops often causes many problems: ranging from the loss of biodiversity, due to the constant cultivation of the same monocultures in the same areas, to the deterioration of soil quality. A way out of this situation is the selection of new untraditional and neglected plants that could grow in a wide range of temperatures, produce high yields and at the same time have a balanced amino acid composition. Pseudocereals of the genus Amaranthus L. meet these criteria. Amaranth grain and plant raw materials are used in many industries: food, medicine, cosmetics. Modern technologies do not stand still. Along with traditional methods of plant breeding, the rapid pace of development involves genetic engineering of plants, which allows the process of creating improved plants to be speeded up several times. The purpose of this study is to analyze and systematize the achievements in the field of regeneration and genetic transformation of representatives of the Amaranthus genus. The results can be used for a practical application: the genetic transformation of species of the genus Amaranthus and other close genera of plants.

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In vitro culture of species and varieties of four Amaranthus L. species

Cited by 20 publications

References 12 publications

In vitro propagation of Alternanthera sessilis (sessile joyweed), a famine food plant

In vitro propagation of Alternanthera sessilis (sessile joyweed), a famine food plant

Diversity in Grain Amaranths and Relatives Distinguished by Genotyping by Sequencing (GBS)

Achievements in Genetic Engineering of Amaranthus L. Representatives

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