“…Bacteria in the form of biofilms cause many infections to humans 14,15 and animals, and they are also a serious concern in the industrial world whenever a water-based process is involved (nautical shipping, paper manufacturing, cooling systems, drinking water facilities, health care, medical devices, and food processing). Therefore, in order to better mimic the natural way of growing, in the present investigation B. multivorans C1576 was cultivated in biofilm mode, using a semipermeable cellulose membrane as solid support, 16 and in non-biofilm mode on agar media. Two different growth media were used: Yeast Extract Mannitol (YEM), 17 which induces the mucoid phenotype in BCC species, and Müller Hinton (MH), which is largely used in microbiological laboratories for different bacterial assays.…”