Dam methylation is required for efficient biofilm production in Salmonella enterica serovar Enteritidis

Castañeda, María del Rosario; Sarnacki, Sebastián H.; Llana, Mariángeles Noto; Guerra, Adriana Gabriela López; Giacomodonato, Mónica N.; Cerquetti, M C

doi:10.1016/j.ijfoodmicro.2014.10.003

Cited by 26 publications

(21 citation statements)

References 78 publications

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“… Aya Castañeda et al (2015) demonstrated that regulation of biofilm formation in S. enterica was dependent on DAM methylation. Mutants lacking the dam gene were unable to produce substantial BC or curli and had reduced csgD and csgA (major structural subunit of curli fimbriae; Aya Castañeda et al, 2015 ) but were unaffected in bcsA expression suggesting a link between DAM methylation and activation of BC biosynthesis by c-di-GMP. The discovery that DAM methylation affects biofilm gene expression is a novel avenue that warrants investigation in all biofilm producers.…”

Section: Animal–bacteria Interactions Of Bc Producersmentioning

confidence: 97%

Establishing a Role for Bacterial Cellulose in Environmental Interactions: Lessons Learned from Diverse Biofilm-Producing Proteobacteria

2015

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Bacterial cellulose (BC) serves as a molecular glue to facilitate intra- and inter-domain interactions in nature. Biosynthesis of BC-containing biofilms occurs in a variety of Proteobacteria that inhabit diverse ecological niches. The enzymatic and regulatory systems responsible for the polymerization, exportation, and regulation of BC are equally as diverse. Though the magnitude and environmental consequences of BC production are species-specific, the common role of BC-containing biofilms is to establish close contact with a preferred host to facilitate efficient host–bacteria interactions. Universally, BC aids in attachment, adherence, and subsequent colonization of a substrate. Bi-directional interactions influence host physiology, bacterial physiology, and regulation of BC biosynthesis, primarily through modulation of intracellular bis-(3′→5′)-cyclic diguanylate (c-di-GMP) levels. Depending on the circumstance, BC producers exhibit a pathogenic or symbiotic relationship with plant, animal, or fungal hosts. Rhizobiaceae species colonize plant roots, Pseudomonadaceae inhabit the phyllosphere, Acetobacteriaceae associate with sugar-loving insects and inhabit the carposphere, Enterobacteriaceae use fresh produce as vehicles to infect animal hosts, and Vibrionaceae, particularly Aliivibrio fischeri, colonize the light organ of squid. This review will highlight the diversity of the biosynthesis and regulation of BC in nature by discussing various examples of Proteobacteria that use BC-containing biofilms to facilitate host–bacteria interactions. Through discussion of current data we will establish new directions for the elucidation of BC biosynthesis, its regulation and its ecophysiological roles.

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Section: Animal–bacteria Interactions Of Bc Producersmentioning

confidence: 97%

Establishing a Role for Bacterial Cellulose in Environmental Interactions: Lessons Learned from Diverse Biofilm-Producing Proteobacteria

2015

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“…Furthermore, Dam mediated the methylation of adenine in the 5′-GATC-3′ sequence shortly after DNA replication, and was implicated as a virulence factor in bacterial pathogenesis. As documented previously, Dam methylation was required for efficient biofilm production in Salmonella enterica serovar enteritidis [18]. Dam was also crucial in modulating the pathogenicity of K. pneumoniae genotype K1 [19].…”

Section: Discussionmentioning

confidence: 62%

Exiguobacterium sp. A1b/GX59 isolated from a patient with community-acquired pneumonia and bacteremia: genomic characterization and literature review

Chen

Wang

Zhou³

et al. 2017

BMC Infect Dis

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BackgroundBacterial species belonging to the genus Exiguobacterium are facultative anaerobic, non-spore-forming, Gram-positive bacilli, and rarely associated with human infections. Herein, we reported the first case of community-acquired pneumonia (CAP) and bacteremia due to Exiguobacterium spp. in China.Case presentationAn adult male with severe CAP was hospitalized. The pathogen was isolated from his bloodstream and broncho-alveolar lavage fluid. The correct identification of the micro-organism was achieved using 16S rRNA sequencing, and its antibiotic susceptibility test was performed by microdilution method. The Whole Genome Sequencing (WGS) was used to characterize its genetic features and to elucidate its potential pathogenic mechanisms. Furthermore, its genome sequence was also compared with those of 3 publicly-available Exiguobacterium strains. A PubMed search was performed for further understanding the features of Exiguobacterium infections. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain GX59 was most closely related to Exiguobacterium AT1b (99.7%). The genome of GX59 was 2,727,929 bp in size, harbouring 2855 putative protein-coding genes, 5 rRNA operons, 37 tRNA genes and 1 tmRNA. The multiple genome comparison of 4 Exiguobacterium strains demonstrated that Exiguobacterium contained 37 genes of secretion systems, including sec, tat, FEA, Type IV Pili and competence-related DNA transformation transporter (Com). Virulence factors of the micro-organism included tlyC, NprR, MCP, Dam, which might play a critical role in causing lethal infection.ConclusionsThe study highlighted the potential pathogenicity of the genus Exiguobacterium for its unique genes encoding various virulence factors and those associated with antibiotic resistance, therefore, its clinical significance should be valued.

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“…In E. coli and its relatives, lack of Dam methylation causes pleiotropic defects, indicating that the multiple DNA-protein interactions are under GATC methylation control 22 23 . DNA methylation has been found to be required in biofilm production as absence of Dam in S. enteritidis affected the ability to form biofilm on polystyrene surfaces 24 25 26 . In addition, it has been reported that a tautomerizing demethylation could disrupt the biofilm formation in anoxic conditions in P. aeruginosa 27 .…”

Section: Introductionmentioning

confidence: 99%

Role of DNA Methylation in Persister Formation in UropathogenicE. coli

Liu

Zhang

et al. 2020

Preprint

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Uropathogenic Escherichia coli (UPEC) persister bacteria play crucial roles in clinical treatment failure and relapse. DNA methylation is known to regulate gene expression in bacteria, but its role in persister formation has not been investigated. Here, we created adenine methylation deletion mutant (Δdam) and cytosine methylation mutant (Δdcm) from UPEC strain UTI89 and found that the Δdam mutant but not Δdcm mutant had significant defect in persister formation during exposure to various antibiotics (gentamicin, fluroquinolones and cephalosporin) and stresses (acid pH and hyperosmosis), and that complementation of the dam mutant restored its persister defect phenotype. PacBio sequencing of epigenetic genomewide methylation signature and RNA sequencing of the Δdam mutant were performed to define, for the first time, the role of adenine methylation in persister formation. Methylome data analysis showed that 99.73% of m6A modifications on GATC were demethylated in the Δdam mutant, and demethylation nucleotide site related genes suggested an overwhelming effect on transcription and metabolic processes. Transcriptome analysis of the Δdam mutant in comparison to wild type showed that flagella biosynthesis, galactitol transport/utilization, and signaling related genes were upregulated while pilus, fimbriae, virulence, glycerol, nitrogen metabolism pathways and transcriptional regulators were downregulated. The comparative COG analysis of methylome and transcriptome enriched pathways identified translation, ribosomal structure and biogenesis, and cell motility were upregulated, whereas DNA repair, secondary metabolite biosynthesis and diverse transport systems, some of which are known to be involved in persister formation, were downregulated in the Δdam mutant. These findings provide new insights about the molecular basis of how DNA adenine methylation may be involved in persister formation and offer novel therapeutic targets for combating persister bacteria.

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Dam methylation is required for efficient biofilm production in Salmonella enterica serovar Enteritidis

Cited by 26 publications

References 78 publications

Establishing a Role for Bacterial Cellulose in Environmental Interactions: Lessons Learned from Diverse Biofilm-Producing Proteobacteria

Establishing a Role for Bacterial Cellulose in Environmental Interactions: Lessons Learned from Diverse Biofilm-Producing Proteobacteria

Exiguobacterium sp. A1b/GX59 isolated from a patient with community-acquired pneumonia and bacteremia: genomic characterization and literature review

Role of DNA Methylation in Persister Formation in UropathogenicE. coli

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