SUDZINA, M., MELICH, M., KŇAZOVICKÁ, V., FELŠÖCIOVÁ, S., KOCIUBINSKI, G., ANDREJI, J., PAVLIČOVÁ, S., KRÁČMAR, S.: Physicochemical characterization of natural honeys from diff erent regions in Slovakia. Acta univ. agric. et silvic. Mendel. Brun., 2009, LVII, No. 2, pp. 125-134 This study is intended to determine the physicochemical characteristics of Slovakian honeys, and compare them with specifi cations described in the Codex Alimentarius Standard, the European Honey directive, the Slovak Codex Alimentarius and Slovak standard No. 1/2006. In addition, we tried to fi nd out correlations between individual constituents of honeys. Physicochemical characterization was carried out following the harmonized methods dictated by the International Honey Commission IHC. Honey samples (n=50) were collected from three honey types (blossom, blends, honeydew), and from the three main Slovakian geographical regions (named 'east', 'middle' and 'west'). The physi co che mi cal evaluation included moisture, reduced sugars, proline, hydroxymethylfurfural (HMF), conductivity, diastase and invertase activity, pH and water activity, following the techniques proposed by the European Honey Commission (EHC). The moisture content in the honey varied from 15.26 to 17.65 %, HMF levels ranged from 21.83 to 63.00 mg . kg , the pH value varied from 4.06 to 4.80 and the water activity content varied from 0.55 to 0.57. Signifi cant diff erences (P ≤ 0.05) were found between HMF and fructose, glucose and saccharose and conductivity and pH, signifi cant differences (P ≤ 0.01) between fructose and glucose and signifi cant diff erences (P ≤ 0.001) were found between HMF and conductivity. Between blossom honey and honeydew honey were found statistical signifi cant diff erences in HMF (P ≤ 0.05) and conductivity between honeydew honey and blossom honey (P ≤ 0.001). Among regions were determined statistical diff erences between HMF and invertase (P ≤ 0.05) and conductivity (P ≤ 0.01).