Grouper TRIM13 exerts negative regulation of antiviral immune response against nodavirus

Abstract

The tripartite motif (TRIM)-containing proteins have attracted particular attention to their multiple functions in different biological processes. TRIM13, a member of the TRIM family, is a RING domain-containing E3 ubiquitin ligase which plays critical roles in diverse cellular processes including cell death, cancer and antiviral immunity. In this study, a TRIM13 homolog from orange spotted grouper, Epinephelus coioides (EcTRIM13) was cloned and characterized. The full-length of EcTRIM13 cDNA encoded a polypeptide of 399 amino acids which shared 81% identity with TRIM13 homolog from large yellow croaker (Larimichthys crocea). Amino acid alignment analysis showed that EcTRIM13 contained conserved RING finger and B-box domain. Expression patterns analysis indicated that EcTRIM13 was abundant in liver, spleen, kidney, intestine and gill. Moreover, the transcript of EcTRIM13 in grouper spleen was differently regulated after injection with Singapore grouper iridovirus (SGIV) or polyinosin-polycytidylic acid (poly I:C). Under fluorescence microscopy, we observed the tubular structure in wild type EcTRIM13 transfected cells, but the RING domain mutant resulted in the fluorescence distribution was changed and the bright punctate fluorescence was evenly situated throughout the cytoplasm, suggesting that the RING domain was essential for its accurate localization. Overexpression of EcTRIM13 in vitro obviously increased the replication of red spotted grouper nervous necrosis virus (RGNNV), and the enhancing effect of EcTRIM13 on virus replication was affected by the RING domain. Furthermore, the ectopic expression of EcTRIM13 not only negatively regulated the interferon promoter activity induced by interferon regulator factor (IRF) 3, IRF7, and melanoma differentiation-associated protein 5 (MDA5), but also decreased the expression of several interferon related factors. In addition, the overexpression of EcTRIM13 also differently regulated the transcription of pro-inflammatory factors. Together, our results firstly demonstrated that fish TRIM13 exerted negative regulation of antiviral response against nodavirus infection.

Advances in the study of nodavirus

“…Tripartite motif-containing (TRIM) proteins are multi-domain proteins that exert important immune regulatory roles on TLR and RLRs mediated antiviral innate immunity ( Huang et al, 2016a ). The innate antiviral immunities of fish against nodavirus infection are summarized in Fig.…”

“…To date, fish TRIM25, TRIM32 and TRIM39 exerted positive antiviral responses via activation of MDA5 expression ( Wang et al, 2016 ; Yang et al, 2016 ; Yu et al, 2017 ). On the other hand, TRIM13 exerted negative regulation on antiviral immunity against nodavirus infection through downregulation of MDA5 and the downstream IFN signaling pathway ( Huang et al, 2016a ).…”

Tripartite motif‐containing proteins precisely and positively affect host antiviral immune response

“…Additionally, TRIM25 induced ubiquitination of RIG‐I and promoted its interactions with MAVS in the RIG‐I signalling pathway, which eventually conferred on the host an inducible I‐IFN immune response. Whereas TRIM62 interacts with TRIF, TRIM5 interacts with TAK1 and TRIM23 and TRIM13 interact with NEMO in the TLR signalling pathway to activate the innate immune response by synthesizing and attaching distinct ubiquitin chains to various substrates.…”

Peer Review #2 of "Advances in the study of nodavirus (v0.2)"

“…However, a recent study showed a contrary function of LGP2, in which the overexpression of LGP2 suppressed the expression of MDA5, Mx promoter, ISRE, pro-inflammatory cytokines and type I interferon genes, thus resulting in a high viral load (Yu et al, 2016). Manuscript to be reviewed (Huang et al, 2016a). The innate antiviral immunities of fish against nodavirus infection are summarized in Fig.…”

“…To date, fish TRIM25, TRIM32 and TRIM39 exerted positive antiviral responses via activation of MDA5 expression (Wang et al, 2016b;Yang et al, 2016;Yu et al, 2017). On the other hand, TRIM13 exerted negative regulation on antiviral immunity against nodavirus infection through downregulation of MDA5 and the downstream IFN signaling pathway (Huang et al, 2016a).…”