Tripartite motif‐containing proteins precisely and positively affect host antiviral immune response

Wei, Yunan; Chen, S; Wang, M; Cheng, Anchun

doi:10.1111/sji.12669

Cited by 10 publications

(8 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several of the multigene families identified are potentially implicated in antiviral immunity. TRIM, one of the most represented multigene family in our sets of DEGs (one third), can target viral proteins for ubiquitination, in association with ubiquitin ligases (also identified here), to inhibit viral replication and induce RLR-STING and TLR-NF-κB signaling pathways, which contribute to antiviral defense [29]. IFI44 multigene family are interferon-alfa inducible proteins, which are associated with infection of several viruses and can affect viral replication [30].…”

Section: Discussionmentioning

confidence: 77%

Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome

et al. 2020

View full text Add to dashboard Cite

Background: As a major threat to the oyster industry, Pacific Oyster Mortality Syndrome (POMS) is a polymicrobial disease affecting the main oyster species farmed across the world. POMS affects oyster juveniles and became panzootic this last decade, but POMS resistance in some oyster genotypes has emerged. While we know some genetic loci associated with resistance, the underlying mechanisms remained uncharacterized. So, we developed a comparative transcriptomic approach using basal gene expression profiles between different oyster biparental families with contrasted phenotypes when confronted to POMS (resistant or susceptible). Results: We showed that POMS resistant oysters show differential expression of genes involved in stress responses, protein modifications, maintenance of DNA integrity and repair, and immune and antiviral pathways. We found similarities and clear differences among different molecular pathways in the different resistant families. These results suggest that the resistance process is polygenic and partially varies according to the oyster genotype. Conclusions: We found differences in basal expression levels of genes related to TLR-NFκB, JAK-STAT and STING-RLR pathways. These differences could explain the best antiviral response, as well as the robustness of resistant oysters when confronted to POMS. As some of these genes represent valuable candidates for selective breeding, we propose future studies should further examine their function.

show abstract

Section: Discussionmentioning

confidence: 77%

Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome

et al. 2020

View full text Add to dashboard Cite

show abstract

“…TRIMs have the E3-ligase activity and played key roles in the E1/E2/E3 ubiquitin-associated multienzyme cascade system. TRIMs can inhibit virus infection by both activating innate immune signaling pathways through ISGs and degradation of virus capsid proteins . A deeper understanding of the TRIMs’ involvement of ISG15 regulation may be helpful in antivirus study.…”

Section: Discussionmentioning

confidence: 99%

“…TRIMs can inhibit virus infection by both activating innate immune signaling pathways through ISGs and degradation of virus capsid proteins. 60 A deeper understanding of the TRIMs' involvement of ISG15 regulation may be helpful in antivirus study. RSAD2 (Viperin), another classical ISG, was upregulated at both levels.…”

Section: ■ Conclusionmentioning

confidence: 99%

Comparative Transcriptomic and Proteomic Analyses Prove that IFN-λ1 is a More Potent Inducer of ISGs than IFN-α against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells

Zhao¹,

Zhai

et al. 2020

J. Proteome Res.

View full text Add to dashboard Cite

Type III interferon (IFN-λ) is currently considered to be largely nonredundant to type I interferon (IFN-α) in antivirus infection, especially in epithelial cells. Previous studies reported that, compared with IFN-α, IFN-λ exhibited stronger induction of interferon-stimulated genes (ISGs) at the transcriptional level in intestinal epithelial cells and stronger inhibition of porcine epidemic diarrhea virus (PEDV). In this study, the different mechanisms of ISG upregulation induced by IFN-α and IFN-λ1 were compared at the mRNA and protein levels in the porcine intestinal epithelial cell model (IPEC-J2). It was proved that IFN-λ1 consistently exhibited stronger stimulation effects at both levels. At the mRNA level, 132 genes were significantly upregulated upon IFN-λ1 stimulation, while 42 genes upon IFN-α stimulation. At the protein level, 47 proteins were significantly upregulated upon IFN-λ1 stimulation, but only 8 proteins were upregulated upon IFN-α stimulation. The shared upregulated genes/proteins by IFN-λ1 in both transcriptional and translational omics, especially the regulation factors of ISG15, were involved in the JAK−STAT signaling pathway. Compared to IFN-α, IFN-λ1 could induce more consistent upregulation of the key ISGs (ISG15, USP18, OASL, and RSAD2) at 3−24 h postinduction as measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation. It was further confirmed through functional analysis that ISG15 and RSAD2 could inhibit PEDV infection in dose-dependent manners. This study provided solid evidence that IFN-λ1 could induce a more unique and higher ISG expression level, which exhibited anti-PEDV effects on porcine intestinal epithelial cells.

show abstract

“…However, rare studies considered this topic. TRIM containing proteins have been shown to play vital roles in modulating cell growth and migration processes (Wei et al 2018). Many TRIM containing proteins are highly expressed in tissues or organs, contributing to the maintenance of normal tissue functions and homeostasis, and thus affect cell growth and migration (Huang et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Tripartite motif containing 28 (TRIM28) promotes the growth and migration of endometrial carcinoma cells by regulating the AKT/mTOR signaling pathway

Chen¹,

Cheng²,

Long³

2023

gpb

View full text Add to dashboard Cite

Although the medical science has been developed for decades, the molecular mechanism of endometrial cancer (EC) is not yet completely clear. Previous studies have shown that the tripartite motif containing 28 (TRIM28) plays a crucial role in tumor development. However, TRIM28 is rarely studied in EC, and its role and mechanism need to be further determined. This study was aimed to delve into the related molecular mechanism underling the role of TRIM28 in EC cell growth and migration. qPCR assays and Western blot assays revealed that the expression level of TRIM28 was higher in EC tissues or cell lines (HEC1B, AN3CA, and Ishikawa) than normal tissue or human endometrial epithelial cells (hEEC), respectively. Then, CCK-8 cell viability assay and clone formation assay were performed in HEC1B and AN3CA cell lines after overexpression or knockdown of TRIM28. The results verified that suppression of TRIM28 expression inhibited the proliferation of EC cells. The wound scratch healing assay and transwell assay were performed in HEC1B and AN3CA cell lines after overexpression or knockdown of TRIM28. The results showed that suppression of TRIM28 expression inhibited the invasion and migration of EC cells. Finally, the Western blot assays hinted that overexpression or knockdown of TRIM28 in HEC1B and AN3CA cell lines would promote or inhibit the phosphorylation of AKT and mTOR protein. These findings indicated that TRIM28 promoted the growth and migration of EC cells via regulating the AKT/mTOR pathway.

show abstract

Tripartite motif‐containing proteins precisely and positively affect host antiviral immune response

Cited by 10 publications

References 86 publications

Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome

Differential basal expression of immune genes confers Crassostrea gigas resistance to Pacific oyster mortality syndrome

Comparative Transcriptomic and Proteomic Analyses Prove that IFN-λ1 is a More Potent Inducer of ISGs than IFN-α against Porcine Epidemic Diarrhea Virus in Porcine Intestinal Epithelial Cells

Tripartite motif containing 28 (TRIM28) promotes the growth and migration of endometrial carcinoma cells by regulating the AKT/mTOR signaling pathway

Contact Info

Product

Resources

About