Group-selective prefractionation and analysis of urinary catecholamines by on-line HPLAC-HPLC Chromatography

Kemper, K.; Hagemeier, E.; Ahrens, Douglas P; Boos, Karl-Siegfried; Schlimme, E.

doi:10.1007/bf02687756

Cited by 13 publications

(2 citation statements)

References 22 publications

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“…Adenosine (Ado), inosine (Ino), guanosine (Guo), uridine (Urd) and cytidine (Cyd) were from Boehringer, Mannheim. N2-dimethylguanosine (m?Guo) and N6-(carbamoyl-threonyl)-adenosine (t 6 Ado) were isolated from urine and characterized by UV-, mass-and ^-NMR-spectroscopy s well s by characteristic chemical reactions; for further characterization t 6 Ado was chemically synthesized in parallel (27).…”

Section: Chemicalsmentioning

confidence: 99%

“…Peaks 8, 10 and 12 were characterized äs rn^no (8), m 2 Guo (10) and miGuo (12) by comparison of retention times of authentic synthetic nucleosides and co-chromatography of authentic nucleosides in a spiked control urine. To prove homogeneity of the Chromatographie zone, peaks 8, 10 and 12 were frequently pooled during the analyses of "water"fractions and additionally characterized by UV-, mass-and ^-spectroscopy äs well äs specific chemical reactions (27). Figure 4c shows the RP-HPLC Separation of a "water "fraction obtained in the clean-up of human serum ultrafiltrate.…”

Section: Hplc-analysis Of Ribonucle Side Fractionsmentioning

confidence: 99%

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Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Hagemeier¹,

Kemper²,

Ks³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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The authors wish to congratulate Professor Walther Lamprecht, Hannover, on the occasion of his sixtieth birthday Summary:We describe an on-line multi-cplumn high performance liquid Chromatographie method for the selective clean-up and analysis of major and minor ribonucleosides in physiological fluids. Quantitative data obtained for the determination of some methylated ribonucleosides in human urines are compared with those obtained with the traditional off-line method.The on-line technique developed in our laboratory is distinguished from the off-line method by the following features:(1) Sample clean-up and analysis of the target-compounds can easily be automatized, (2) Total time of analysis, for example of urinary ribonucleosides, is decreased to 35 minutes, (3) Laborious and error-prone evaporation and redissolution Steps are avoided, (4) Reliability of the overall analytical System can be controlled with ease, (5) Small sample-volumes can be applied directly, (6) Sensitive samples can be processed very rapidly under mild conditions, (7) Results obtained with the -line and off-line-techniques compare well. Hagemeier, Kemper, Boos and Schlimme: Quantitative determination of minor ribonucleosides (5) Kleine Probenvolumina können direkt appliziert werden, (6) Empfindliche Proben können in kürzester Zeit unter schonenden Bedingungen analysiert werden, (7) Die mit der "on-line" wie mit der "off-line"-Technik erhaltenen Ergebnisse zeigen eine gute ÜbereinŜ timmung. Entwicklung einer chromatographischen Methode zur quantitativen Bestimmung seltener Ribonucleoside in physiologischen Flüssigkeiten Charakterisierung und quantitative Bestimmung von seltenen Ribonucleosiden in physiologischen Flüssig

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Section: Chemicalsmentioning

confidence: 99%

Section: Hplc-analysis Of Ribonucle Side Fractionsmentioning

confidence: 99%

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Hagemeier¹,

Kemper²,

Ks³

et al. 1984

Clinical Chemistry and Laboratory Medicine

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Determination of biogenic amine neurotransmitters by RP‐HPLC/FL method on Chromolith RP18e stationary phase

Baranowska

Zydroń

2003

J of Separation Science

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The Chromolith RP-18e stationary phase (Merck, Darmstadt, Germany) was tested for the separation of biogenic amine neurotransmitters and their derivatives. Citrate, acetate, and phosphate buffer solutions with methanol and acetonitrile as modifiers were used as the mobile phases. The tested column proved its high efficiency for the separation of the compounds of interest, resulting in short retention times and permitting working with low pressure. Three gradient systems were developed. The best results were obtained for acetate buffer solution with methanol as the modifier. The system was applied to the analysis of urine samples.

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Affinity Chromatography with Immobilized Benzeneboronates

Beneš

Štambergova

Scouten

1993

Molecular Interactions in Bioseparations

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Group-selective prefractionation and analysis of urinary catecholamines by on-line HPLAC-HPLC Chromatography

Cited by 13 publications

References 22 publications

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Development of a Chromatographic Method for the Quantitative Determination of Minor Ribonucleosides in Physiological Fluids. Characterization and quantitative determination of minor ribonucleosides in physiological fluids, Part I

Determination of biogenic amine neurotransmitters by RP‐HPLC/FL method on Chromolith RP18e stationary phase

Affinity Chromatography with Immobilized Benzeneboronates

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