Group II subfamily secretory phospholipase A₂ enzymes: expression in chronic rhinosinusitis with and without nasal polyps

Abstract

The expression of some members of group II subfamily of sPLA(2)s is upregulated in CRS and it may result from IL-1beta and TNF-alpha overexpression. Different individual group II subfamily sPLA(2)s may play different roles in the pathogenesis of CRS with and without NPs.

Distinct immunopathologic characteristics of various types of chronic rhinosinusitis in adult Chinese

“…DC-LAMP was detected by using the streptavidin-peroxidase complex method with a histostain-plus kit (Boster Biotechnology, Wuhan, China) as described previously. 13 Color development was achieved with 39,39-diaminobenzidine, which rendered positive cells brown. Finally, sections were counterstained with hematoxylin and mounted.…”

Association of chronic rhinosinusitis with nasal polyps and asthma: Clinical and radiological features, allergy and inflammation markers

“…For example, interleukin (IL-5), an eosinophil survival and differentiation factor, and eosinophil cationic protein (ECP), eotaxin, the indicators for eosinophil chemotaxis and activation, Ig E, have been found to be significantly increased in NP vs. CRS and controls (7)(8)(9)(10)(11)(12). Expression of cytokines, metalloproteinases and their inhibitors, proinflammatory enzymes (group II subfamily secretory phospholipase A2) may play different roles in the pathogenesis of CRS with and without NPs (7,9,13,14).…”

Clara Cell 10-kD Protein Suppresses Chitinase 3-Like 1 Expression Associated with Eosinophilic Chronic Rhinosinusitis

“…cDNA was reverse transcribed as stated elsewhere (7,19). By using the specific primer pairs described in Table E2 in the online supplement and SYBR Premix Ex Taq kit (TaKaRa Biotechnology, Dalian, China), cDNA equivalent to 40 ng total RNA was used to perform quantitative PCR as mentioned elsewhere (19). Relative gene expression was calculated by using the comparative CT method (7,8,19).…”

“…By using the specific primer pairs described in Table E2 in the online supplement and SYBR Premix Ex Taq kit (TaKaRa Biotechnology, Dalian, China), cDNA equivalent to 40 ng total RNA was used to perform quantitative PCR as mentioned elsewhere (19). Relative gene expression was calculated by using the comparative CT method (7,8,19). Glyceraldehyde-3-phosphate dehydrogenase (for human and BEAS-2B cell line samples) and b-actin (for mouse samples) were used as housekeeping genes for normalization and a ''no template'' sample was used as a negative control.…”