Group B Streptococci in the Human Population

Jelínková, J

doi:10.1007/978-3-642-66653-7_4

Cited by 45 publications

(48 citation statements)

References 147 publications

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“…Strains 8/66 and 24/60 were used for the preparation of antisera to the group polysaccharide and to protein X, respectively. Antisera were elicited in rabbits according to the modified immunization scheme of Jelinkova (1977). A single dose (0.5 ml) was given, and then 7 d later three doses were administered on three successive days for 4 weeks.…”

Section: Methodsmentioning

confidence: 99%

Immunoelectron Microscopic Study of the Location of Group-Specific and Protein Type-Specific Antigens of Group B Streptococci

Wagner¹,

Wagner²,

Kubín³

et al. 1980

Microbiology

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95The ultrastructural location of the group-specific polysaccharide and the type-specific protein antigens R and X of group B streptococci was studied by means of the direct immunoferritin technique. The group-specific antigen was located on the outer wall layer. The specificity of the reaction was proved by the inhibition of labelling after absorption of the antibody-ferritin conjugate with group B polysaccharide. On the other hand, the demonstration of the polysaccharide was not sterically hindered by protein type antigens. As with group A and C streptococci the group polysaccharide could be localized on both the outer and inner surfaces of isolated walls. The protein antigens R and X were also demonstrated on the wall surface. The specificity of the reaction was ensured by making use of the enzymic sensitivity of these antigens. The location of the R protein on long filaments protruding from the cell surface resembles that of M protein of group A streptococci. In contrast to the group polysaccharide both the R and X protein antigens are localized only on the outer surface of isolated walls.

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Section: Methodsmentioning

confidence: 99%

Immunoelectron Microscopic Study of the Location of Group-Specific and Protein Type-Specific Antigens of Group B Streptococci

Wagner¹,

Wagner²,

Kubín³

et al. 1980

Microbiology

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“…III and non-typable by the GP method. The presence of crossreacting protein antigens such as the X and R antigens described by Jelinkova (1977) and Wilkinson (1975) may have been responsible for discrepant Ib and 111 type allocations but was not proved. When strains classed as non-typable by GP were allocated to types by other methods, we cannot be certain which result should be viewed as wrong.…”

Section: Resultsmentioning

confidence: 94%

“…Other cross-reacting serotypes (Il/Ib, II/Ic etc.) are occasionally found and a small percentage of human isolates are nontypable (Jelinkova, 1977). The serotype classification is also significant because immunity to group B streptococci appears to be type-specific, certain serotypes appear more virulent than others and immunotherapy has been proposed for certain infections (Shigeoka, Hall and Hill, 1978).…”

Section: Introductionmentioning

confidence: 99%

“…The classical method for serotyping is the Lancefield precipitin test (Lancefield, 1938), or the modification of Jelinkova (1977), which is slow, relatively complex and not always easy to interpret. Other methods that have been used and offer various advantages are immunofluorescence (Romero and Wilkinson, 1974), CIEP (Hill et al 1974), MID (Cropp et al, 1974) and COA (Kirkegaard and Field, 1977).…”

Section: Introductionmentioning

confidence: 99%

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A Comparison of Four Methods for the Serotyping of Group B Streptococci

Triscott

Davis

1979

Aust J Exp Biol Med

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Summary. Group B streptococci are implicated in a wide range of clinical conditions in human adults and neonates. The Group is subdivided into five serotypes Ia, Ib, Ic, n and III, which are differentiated on the basis of capsular polysaccharides. In the interests of epidemiology and efficiency a cheap, rapid method which is easily interpreted would be advantageous. In this study four methods of serotyping, namely, counterimmunoelectrophoresis (CIEP), microimmunodiffusion (MID), coagglutination (COA), and the Lancefield capillary precipitin (CP) test were compared in terms of ease of operation and interpretation, accuracy and rapidity. Todd Hewitt Broth (THB) cultures and acid extracts of the group B streptococcal strains were used as antigens for these methods. It was concluded that COA using THB cultures allows cheap and rapid screening for presumptive serotyping, having a 93-96% correlation with the CP test. MID gives an accurate (100% correlation with the CP test) and unambiguous confirmatory diagnosis of serotype.

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“…Jensen (1980) found R-protein in 93% of type I11 strains from the female urogenital tract in Denmark whereas Jelinkova (1977) reported that only 16% of human type I11 isolates in Czechoslovakia contained R-protein; the differences in results might be ascribable to mucosal adherence because the strains reported by Jelinkova were unselected. However, the present results clearly indicate that R-protein might contribute to the capacity of GBS type 111 to colonise the mucosal membranes.…”

Section: Discussionmentioning

confidence: 98%

Colonisation of the upper respiratory tract of mice with group B streptococci type III with reference to the R-protein

Kurl

Christensen

1984

Journal of Medical Microbiology

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SUMMARY. Nine strains of group B streptococci type 111, five with R-protein ( R + ) and four without (R-) were tested for capacity to colonise the upper respiratory tract in mice and to adhere to human buccal cells. In the mouse model, 80-pl inocula of dilutions of overnight cultures of the strains in Todd Hewitt broth were placed in the external nares under light ether anaesthesia. A pilot experiment demonstrated that it was reasonable to study the throat colonisation 2 and 4 days after inoculation. Groups of 18-20 mice were then given inocula containing 8 x lo6 cfu/ml of five R + and four R -strains. At day 4, significantly more mice were colonised with type 111, R + strains (73% of the animals) than with type 111, R-strains (44%) (p

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Group B Streptococci in the Human Population

Cited by 45 publications

References 147 publications

Immunoelectron Microscopic Study of the Location of Group-Specific and Protein Type-Specific Antigens of Group B Streptococci

Immunoelectron Microscopic Study of the Location of Group-Specific and Protein Type-Specific Antigens of Group B Streptococci

A Comparison of Four Methods for the Serotyping of Group B Streptococci

Colonisation of the upper respiratory tract of mice with group B streptococci type III with reference to the R-protein

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