A Comparison of Four Methods for the Serotyping of Group B Streptococci

Triscott, Mark; Davis, G. H. G.

doi:10.1038/icb.1979.53

Cited by 8 publications

(6 citation statements)

References 8 publications

(10 reference statements)

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“…Many GBS capsular polysaccharide typing methods have been described (1,5,10,14,18,35,36), with the most common methods based on serological tests, i.e., immunodiffusion and commercial latex agglutination methods. Other PCR-based molecular methods for typing GBS strains have also been described (19,23,24).…”

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confidence: 99%

International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (Group B Streptococci)

Afshar¹,

Broughton²,

Creti

et al. 2011

J Clin Microbiol

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We report the results from the first international multicenter external quality assessment (EQA) studies for molecular and serological typing of group B streptococcus (GBS) strains as part of DEVANI (Design of a Vaccine against Neonatal Infections), a pan-European program. A questionnaire-based surveillance was undertaken among eight laboratories participating in DEVANI and six laboratories not participating in DEVANI from 13 countries in order to assess their current microbiological procedures for GBS screening, diagnosis, and typing. GBS strains from three EQA distributions were characterized using molecular and serological methods based on GBS capsular polysaccharide typing. Participants were asked to test the first distribution using their current serotyping and genotyping methods. The Strep-B-Latex agglutination method was the most widely used method, with a typeability value of >90%. A multiplex PCR assay for GBS capsular gene typing was also used by 2 of 14 centers, which achieved a typeability value of 93%; this assay detected only 9 of 10 GBS capsular polysaccharide genes. From the second and third EQA studies, standardized protocols were prepared for serological and molecular typing of GBS strains based on the Strep-B-Latex agglutination method and a novel multiplex PCR assay that detected all 10 GBS capsular types (Ia to IX). These standardized protocols are being used by many European laboratories, and as the use of these methods increases, it is imperative to continuously improve and assess laboratory performance and offer training to any laboratories that have technical difficulties.

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confidence: 99%

International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (Group B Streptococci)

Afshar¹,

Broughton²,

Creti

et al. 2011

J Clin Microbiol

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“…Many GBS serotyping methods have been reported previously, including immunoprecipitation (26), enzyme immunoassay (14), coagglutination (11), counterimmunoelectrophoresis and capillary precipitation (24), latex agglutination (27), fluorescence microscopy (6), and inhibition enzyme-linked immunosorbent assay (1), and antisera are necessary for most of these methods. Commercial antisera are expensive and available for only six serotypes (Ia to V), which does not satisfy the needs of traditional serotyping methods (1).…”

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confidence: 99%

Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus ( Streptococcus agalactiae )

Wen

Wang

et al. 2006

J Clin Microbiol

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Group B Streptococcus (GBS; Streptococcus agalactiae) is an important cause of sepsis and meningitis. Nine GBS serotypes, based on capsular polysaccharide (CPS) antigens, have been described. Their distribution varies worldwide and needs to be monitored to understand the epidemiology of GBS disease and inform the development of vaccines. In this study, we sequenced cpsH of GBS serotype II (cpsHII) and compared it with that of the other eight serotypes to identify serotype-specific regions. We then developed a DNA microarray based on the cpsH gene and used it to test 88 GBS isolates-9 serotype reference strains and 79 clinical isolates-and 7 other bacterial and fungal species which are commonly present in the vagina flora. The microarray was shown to be specific and reproducible. This is the first report of a microarray which can identify the nine GBS serotypes. The use of a microarray has advantages over traditional serotyping methods and will be of practical value in both reference and diagnostic laboratories.

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“…Various serotyping methods have been used, including immunoprecipitation (29), enzyme immunoassay (10), coagglutination (6), counterimmunoelectrophoresis and capillary precipitation (27), latex agglutination (31), fluorescence microscopy (5), and inhibition enzyme-linked immunosorbent assay (2). These methods are labor-intensive and require high-titer serotype-specific antisera, which are expensive and difficult to make and which are commercially available for only six serotypes, serotypes Ia to V (2).…”

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confidence: 99%

Serotype Identification of Group B Streptococci by PCR and Sequencing

et al. 2002

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Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution will be needed to guide the development and use of GBS conjugate vaccines. We designed sequencing primers based on the previously published sequences of the capsular polysaccharide (cps) gene clusters to further define partial cps gene clusters for eight of the nine GBS serotypes (serotypes Ia to VII). Subsequently, we designed and evaluated primers to identify serotypes Ia, Ib, III, IV, V, and VI directly by PCR and all eight serotypes (serotypes Ia to VII) by sequence heterogeneity. A total of 206 clinical GBS isolates were used to compare our molecular serotype (MS) identification method with conventional serotyping (CS). All clinical isolates were assigned an MS, whereas 188 of 206 (91.3%) were assigned a serotype by use of antisera. A small number of isolates (serosubtypes III-3 and III-4) showed different serotype specificities between PCR and sequencing, but the PCR results correlated with those obtained by CS. The overall agreement between the MS identification method and CS for isolates for which results of both tests were available was 100% (188 of 188 isolates). The MS identification method is a specific and practical alternative to conventional GBS serotyping and will facilitate epidemiological studies.

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A Comparison of Four Methods for the Serotyping of Group B Streptococci

Cited by 8 publications

References 8 publications

International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (Group B Streptococci)

International External Quality Assurance for Laboratory Identification and Typing of Streptococcus agalactiae (Group B Streptococci)

Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus ( Streptococcus agalactiae )

Serotype Identification of Group B Streptococci by PCR and Sequencing

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