Group 2 innate lymphoid cells constrain type 3/17 lymphocytes in shared stromal niches to restrict liver fibrosis

Abstract

Group 2 innate lymphoid cells (ILC2s) cooperate with adaptive Th2 cells as key organizers of tissue type 2 immune responses, while a spectrum of innate and adaptive lymphocytes coordinate early type 3/17 immunity. Both type 2 and type 3/17 lymphocyte associated cytokines are linked to tissue fibrosis, but how their dynamic and spatial topographies may direct beneficial or pathologic organ remodelling is unclear. Here we used volumetric imaging in models of liver fibrosis, finding accumulation of periportal and fibrotic tract IL-5+ lymphocytes, predominantly ILC2s, in close proximity to expanded type 3/17 lymphocytes and IL-33-high niche fibroblasts. Ablation of IL-5+ lymphocytes worsened carbon tetrachloride- and bile duct ligation-induced liver fibrosis with increased niche IL-17A+ type 3/17 lymphocytes, predominantly yd T cells. In contrast, concurrent ablation of IL-5+ and IL-17A+ lymphocytes reduced this progressive liver fibrosis, suggesting a cross-regulation of type 2 and type 3 lymphocytes at specialized fibroblast niches that tunes hepatic fibrosis.

Dynamic fibroblast-immune interactions shape wound healing after brain injury

“…83 To calculate fibroblast TGF β scores, we utilized a previously generated bulk sequencing dataset of primary lung fibroblasts treated with TGF β (1ng/mL) or PBS for 48 hours. 84 TGF β -upregulated genes were identified from normalized data (log2FC > 0, q < 0.05). Module scores were generated using Seurat’s AddModuleScore function.…”