Gross vascularization of experimentally induced transplanted tumors of the central and peripheral nervous system

Pakula, H; Hd, Mennel; Kj, Zülch

doi:10.1007/bf00685014

Cited by 16 publications

(1 citation statement)

References 5 publications

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“…Other investigators have used static imaging techniques including corrosion casting [21], microfocal radiography [22] and serial reconstruction [23]. Dynamic in vivo methods include transparent chambers [24] and corneal implants [15]. These allow visualization of the dynamic processes but do not lend themselves to precise quantitation of topography and geometry of the vasculature.…”

Section: Discussionmentioning

confidence: 99%

A new glioma model in rat: The C6 spheroid implantation technique permeability and vascular characterization

1987

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The C6 spheroid implantation glioma model is a simple, easily reproduced model for primary gliomas in which C6 astrocytoma cells are grown in vitro as spheroids and subsequently implanted into the brains of Sprague Dawley rat hosts. This report describes the growth, histology, vessel architecture and vascular permeability of the resulting tumors. The appearance of the tumor was investigated by light and electron microscopy, and by using the alkaline phosphatase technique. The leakage of tracer was measured from vessels in the tumor and peritumoral area at various times during tumor development. The spheroid implant produces a fully vascularized, rapidly growing tumor with many of the characteristics of glioblastoma multiforme, from an avascular focus of neoplastic cells. The major advantage of this model is that the tumors grow in a spheroidal fashion and the tumor-brain interface can be easily located. Many of the important events in the process of vascularization take place at the tumor-brain interface. Two distinctive vascular events appear to occur simultaneously: proliferation of blood vessels and their growth into the tumor mass so that they develop into typical, permeable tumor vessels, and migration of tumor cells along normal vessels into the surrounding brain. Tumor vessels were permeable to the tracer Evans Blue (EB) from the earliest days of ingrowth. Leakage of the EB increased as the tumors increased in size, but eventually leakage plateaued as tumors developed necrotic centers. It is well known that the structural and permeability characteristics of vessels associated with the tumor affect tumor growth. This model will be useful for a number of proposed studies including assessment of various clinical therapies on tumor growth and development, and more specifically, quantitative analysis of the vascularization process in tumors.

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Section: Discussionmentioning

confidence: 99%

A new glioma model in rat: The C6 spheroid implantation technique permeability and vascular characterization

1987

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In Vivo ³¹P and ¹H NMR studies of rat brain tumor pH and blood flow during acute hyperglycemia: Differential effects between subcutaneous and intracerebral locations

Ross

Mitchell

Merkle

1989

Magnetic Resonance in Med

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Surface coil NMR spectroscopy was used to monitor the hyperglycemia-induced alterations in pH and blood flow in vivo in C6 gliomas implanted both subcutaneously and intracerebrally in rats. Tumor pH was calculated from the chemical shift difference between PCr and Pi in the 31P NMR spectra. Subcutaneous glioma pH decreased 0.8 units by 1 h after intraperitoneal administration of an aqueous 50% glucose solution (6 g glucose per kg body weight). In contrast, hyperglycemia failed to significantly alter the pH of intracerebral gliomas which were monitored for 90 min following administration of glucose. Tumor blood flow (TBF) was determined both pre- and post-glucose administration using deuterium NMR by monitoring the time course of D2O washout following intratumoral injection of saline D2O. Subcutaneous and intracerebral TBF were found to have an average change of -78.1% (range -47.4 to -93.3%, n = 5) and -21.1% (range +6.0 to -37.8%, n = 9), respectively. In addition, laser Doppler blood flow measurements of rat skin and subcutaneous glioma revealed a dramatic reduction in blood flow in both tissues following glucose administration. These results indicate that the effects of acute hyperglycemia are site dependent and that hyperglycemia alone is not beneficial for inducing intracellular acidosis in intracerebral tumors.

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Neuropathologie

Peiffer¹

1984

Pathologie

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Gross vascularization of experimentally induced transplanted tumors of the central and peripheral nervous system

Cited by 16 publications

References 5 publications

A new glioma model in rat: The C6 spheroid implantation technique permeability and vascular characterization

A new glioma model in rat: The C6 spheroid implantation technique permeability and vascular characterization

In Vivo ³¹P and ¹H NMR studies of rat brain tumor pH and blood flow during acute hyperglycemia: Differential effects between subcutaneous and intracerebral locations

Neuropathologie

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Gross vascularization of experimentally induced transplanted tumors of the central and peripheral nervous system

Cited by 16 publications

References 5 publications

A new glioma model in rat: The C6 spheroid implantation technique permeability and vascular characterization

A new glioma model in rat: The C6 spheroid implantation technique permeability and vascular characterization

In Vivo 31P and 1H NMR studies of rat brain tumor pH and blood flow during acute hyperglycemia: Differential effects between subcutaneous and intracerebral locations

Neuropathologie

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In Vivo ³¹P and ¹H NMR studies of rat brain tumor pH and blood flow during acute hyperglycemia: Differential effects between subcutaneous and intracerebral locations