Grooved Titanium Surfaces Orient Growth and Migration of Cells from Human Gingival Explants

Brunette, D. M.; Kenner, Gail S.; Gould, Timothy

doi:10.1177/00220345830620100701

Cited by 170 publications

(90 citation statements)

References 8 publications

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“…However, the effect of cylindrical topography on cell nuclei alignment disappeared when fiber diameter was bigger than 200 μm. Brunette et al (Brunette et al, 1983) studied outgrowth of human gingival explants on a titanium surface etched with trapezoid shape grooves (groove upper width 130 μm and lower width 60 μm, ridge width 10 μm). The direction of outgrowth was strongly guided by the grooves.…”

Section: Tissue Subunits (Submillimeter) Scale Topographymentioning

confidence: 99%

Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds

Wang¹

2011

Advances in Biomimetics

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Section: Tissue Subunits (Submillimeter) Scale Topographymentioning

confidence: 99%

Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds

Wang¹

2011

Advances in Biomimetics

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“…72 " 74 Thus, it appears that the direction of cellular outgrowth from a source (e.g., tissue explants) can be guided by the topology of the substratum and this has been demonstrated with grooved titanium surfaces. 73 Preliminary studies using percutaneous implants have shown that grooving the surface can decrease the length and rate of epithelial migration. 7375 Although the research in this area is revealing important information, very little is known about the effects of changing surface topography of substrata on the proliferation and matrix synthetic profiles of cells.…”

Section: Regeneration Of Connective Tissue Attachment To Previousmentioning

confidence: 99%

Experimental Regeneration of The Periodontium

Aukhil

Nishimura

Fernyhough

1990

Critical Reviews in Oral Biology & Medicine

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“…[1][2][3][4][5][6][7] Although various, often ingenious methods have been used to produce different topographies for studies of cell behaviors, the introduction of substrata produced by micromachining enabled a more precise control of substrata geometry to be achieved. 8 Micromachined substrata have now been used in diverse studies of cell and cytoskeletal behavior in vitro 2,4,5,9 and as implant surfaces in vivo. 10,11 A consistent finding is that grooved micromachined substrata alter cell shape and cytoskeletal organization.…”

Section: Introductionmentioning

confidence: 99%

Effects of titanium substratum and grooved surface topography on metalloproteinase-2 expression in human fibroblasts

Chou

Firth

Uitto

et al. 1998

J. Biomed. Mater. Res.

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The chemical and topographic effects of commercially pure titanium on cell morphology and the regulation of matrix metalloproteinase-2 (MMP-2) gene expression, synthesis, and activity were investigated in early passage human gingival fibroblasts. Scanning electron microscopy showed that on smooth titanium (Ti), fibroblasts remained well spread and randomly oriented throughout the culture period. In contrast, cells on V-shaped grooved titanium (VTi) were oriented along the grooves by 16 h and proliferated in this organization throughout the culture period. The effects of substratum surface chemistry on MMP-2 expression were found to be distinct from those of topography. Northern hybridization analysis of fibroblasts cultured on Ti revealed an MMP-2 mRNA time-course expression pattern parallel to that observed on the tissue culture plastic (TCP) dishes, but at significantly lower levels at each time-point. The Ti mRNA levels were decreased by 34% at 16 h, 55% at 40 h, and 45% at 90 h relative to TCP. In contrast, MMP-2 mRNA expression on VTi showed both an altered time-course expression pattern and altered levels compared to Ti and TCP. Relative to TCP, VTi MMP-2 mRNA levels were approximately 80% less at 16 h and approximately 50% less at 40 h, but not significantly different at 90 h. Relative to Ti, VTi MMP-2 mRNA levels were approximately 75% less at 16 h, but approximately 40% greater at 40 h and approximately 70% greater at 90 h. These differences may be explained in part by the observed changes in MMP-2 mRNA half-life which decreased by approximately 40% on Ti but increased by over fourfold on VTi relative to TCP. The smooth Ti also showed an approximate twofold increase of MMP-2 secretion in the late cultures over TCP controls. These results indicate that substratum surface chemistry and topography-induced changes in cell shape can alter MMP-2 expression in normal fibroblasts. The molecular approach to investigating the major molecules involved in tissue degradation may provide sensitive indicators of tissue remodeling at the tissue-biomaterial interface.

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Grooved Titanium Surfaces Orient Growth and Migration of Cells from Human Gingival Explants

Cited by 170 publications

References 8 publications

Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds

Biomimetic Topography: Bioinspired Cell Culture Substrates and Scaffolds

Experimental Regeneration of The Periodontium

Effects of titanium substratum and grooved surface topography on metalloproteinase-2 expression in human fibroblasts

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