Green tea extract attenuates non alcoholic fatty liver disease by decreasing hyperlipidemia and enhancing Superoxide dismutase activity in cholesterol-fed rats

Abstract

BACKGROUND/AIM: Health benefits of green tea for a wide variety of ailments, including the cancer, heart disease, and liver disease, were reported. It is believed to have beneficial effects in the prevention and treatment of many diseases, one of which is non-alcoholic fatty liver disease (NAFLD). This study inspects the protective effect of green tea against atherosclerosis and NAFLD in comparative approach between curative and preventive models. MATERIALS AND METHODS: Twenty four of Wistar rats were studied for 150 days. After 15 days of adaptation period, rats were divided into four groups including normal Group (NG), control Hypercholesterolemic diet Group (CHDG), preventive Group (PG) and curative Group (CG) that followed respectively the following regimens: 1 mL/kg of sunflower oil for 150 days, 1 mL/kg of cholesterol solution prepared at 1.5% (w/v) in sunflower oil span 150 days, 1 ml/kg of cholesterol solution at 1.5% (w/v) in sunflower oil with 3 mL/kg GTLE for 60 days and 1 mL/kg of cholesterol solution at 1.5% (w/v) in sunflower oil for 30 days followed by 3 mL/kg of GTLE for 30 days. These both PG and CG groups were ingested with cholesterol 1.5% (w/v) during remaining period. RESULTS: The results showed significant increase, except for NG, during the 30 first days (p≤0.001) in lipid serum profiles including Total Cholesterol (TC), Triacylglycerol (TG) and Low-Density Lipoprotein cholesterol (LDL-c). However, the High-Density Lipoprotein cholesterol (HDL-c) profile decreased during the treatment (p≤0.001). The ingestion of GTLE in treated groups (CG and PG) declined significantly (p≤0.001) in blood lipid concentrations (TC: 67%, TG: 23%, LDL-c: 81.12%) except for the HDL-c that increased up to 15%. The Atherogenic Index (AI) also decreased significantly (p≤0.001) up to 48%, in CG and PG. PG and NG marked same SOD activity values (130.91±7.66 versus 141.31±8.21 U/mL), while CG showed the lowest level. Liver sections were well protected in protective model than curative one.

Plant‐Based Foods and Their Bioactive Compounds on Fatty Liver Disease: Effects, Mechanisms, and Clinical Application

“…The aqueous extract of green tea alleviated NAFLD in C57BL/6 mice by modulating the expression of miR-34a and miR-194, resulting in increasing the expression of SIRT1, PPAR- α , and insulin-induced gene 2 (Insig2), as well as decreasing the expression of apolipoprotein a5, 3-hydroxy-3-methyl glutaryl coenzyme A synthase (HMG-CoA synthase), and HMG-CoA reductase [ 110 ]. Also, the consumption of the green tea extract alleviated hyperlipidemia and enhanced the activity of superoxide dismutase (SOD) in Wistar rats [ 113 ]. Moreover, oral gavage of green tea polyphenols could inhibit the hepatic lipogenesis in Zucker rats by upregulating the AMPK signaling pathway, with reducing the levels of insulin, glucose, ALT, AST, TNF- α , and interleukin-6 (IL-6) [ 114 ].…”

“…Oxidative Medicine and Cellular Longevity dismutase (SOD) in Wistar rats [113]. Moreover, oral gavage of green tea polyphenols could inhibit the hepatic lipogenesis in Zucker rats by upregulating the AMPK signaling pathway, with reducing the levels of insulin, glucose, ALT, AST, TNF-α, and interleukin-6 (IL-6) [114].…”

Effect of stevia and pectin supplementation on physicochemical properties, preservation and in‐vivo hypoglycemic potential of orange nectar

“…Total titratable acidity (TTA) was determined according to Wibowo et al (2015). Total sugar contents expressed as (mg/l) were determined according to Labdi et al (2018), calibration curve made of different solutions of glucose in water varying from 100 mg/L to 1,000 ml/l was used for the measure the results were extrapolated according the equation ( y = 0.0005 * x −0.0016, R 2 = .996).…”

Gambir (Uncaria gambir Roxb.) as A Potential Alternative Treatment for Hyperlipidemia

“…28 Several other studies were by Suzuki et al reported methylated catechins direct the strong lipid-lowering activity in mice; Labdi et al confirmed the protective role of green tea intake in against atherosclerosis on hypercholesterol-fed rat; Ahmad et al reported the drinks supplemented with catechins and EGCG are effective against obesity, hypercholesterolemia and hyperglycemia in rats. 29,30,31…”