Green Tea (Camelia Sinensis) Suppresses B Cell Production Of IgE Without Inducing Apoptosis

Hassanain, E.; Silverberg, Jonathan I.; Norowitz, Kevin B.; Chice, Seto; Wright, L.; Forgy, C. Connan; Bluth, Martin H.; Brody, Neil; Joks, Rauno; Durkin, Helen G.; Smith‐Norowitz, Tamar A.

doi:10.1016/j.jaci.2009.12.079

Cited by 17 publications

(18 citation statements)

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“…One of the active principles was identified as dimethylmatairesinol. Many natural extracts have been shown to suppress IgE production by U266 cells, [2][3][4][5][6] but these studies failed to identify the active principle. For example, green tea extract has been shown to suppress IgE production in U266 cells, but the structure of the active component(s) remains to be determined.…”

Section: Resultsmentioning

confidence: 99%

“…[1][2][3][4][5][6] We have used these cells to evaluate suppressive effect of IgE production or secretion and examined methanol extracts from more than 200 species of plant. As a result, we found that a methanol extract from leaves of the camphor tree Cinnamomum camphora (C. camphora Lauraceae) decreased the amount of IgE in the culture medium of U266 cells dose-dependently.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Dimethylmatairesinol as an Immunoglobulin E-suppressing Component of the Leaves of Cinnamomum camphora

Tanabe

Fukutomi

Yasui

et al. 2011

JOURNAL OF HEALTH SCIENCE

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Immunoglobulin E (IgE) plays an important role in allergic diseases. In this study, we found that a methanol extract of leaves of the camphor tree Cinnamomum camphora (C. camphora) reduced the amount of IgE secreted by human myeloma U266 cells. When the methanol extract was fractionated by extraction with organic solvents, the ethyl acetate fraction showed the highest activity. The fraction was further separated into several subfractions by preparative TLC. We identified the component of one of the active subfractions as dimethylmatairesinol. Authentic dimethylmatairesinol exhibited similar activity. Thus, the extract of C. camphora and its components including dimethylmatairesinol have potential as an anti-allergic agent.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Dimethylmatairesinol as an Immunoglobulin E-suppressing Component of the Leaves of Cinnamomum camphora

Tanabe

Fukutomi

Yasui

et al. 2011

JOURNAL OF HEALTH SCIENCE

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“…7,8,10 Namun, hasil penelitian ini berbeda dengan penelitian lainnya yang dilakukan oleh Hassanain dkk. 13 di New York tentang peran teh hijau secara in vitro dengan melakukan kultur terhadap sel yang menyekresi IgE selama 72 hari. Penelitian dilakukan pada 2 kelompok, yaitu kelompok yang diberi ekstrak teh hijau dan kelompok tanpa ekstrak teh hijau.…”

Section: Pembahasanunclassified

“…13 Suhu pemanasan ekstrak itu ternyata sangat memengaruhi kandungan catechin yang ada dalam teh hijau. 12,13 Kandungan catechin yang paling tinggi yaitu pada suhu 118,1°C dibanding dengan suhu 50°C, 70°C, dan 90°C. Ekstrak dalam penelitian ini dipanaskan pada suhu 45°C sehingga kemungkinan hasil kandungan catechin yang diperoleh hanya sedikit.…”

Section: Pembahasanunclassified

Aktivitas Polifenol Teh Hijau (Camellia sinensis (L) O. Kuntze) Sebagai Imunomodulator melalui Respons Supresi Imunoglobulin E (IgE) pada Rinitis Alergika

Yusni¹,

R²,

Achmad³

2015

mkb

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AbstrakRinitis alergika adalah kondisi inflamasi mukosa nasal yang diakibatkan oleh interaksi antara alergen dan imunoglobulin E (IgE). Imunomodulator merupakan bagian terpenting dalam pengobatan rinitis alergika dan salah satu tanaman obat yang mempunyai aktivitas imunomodulator adalah teh hijau (Camellia sinensis L.) terutama polifenol. Tujuan penelitian adalah menganalisis aktivitas polifenol teh hijau sebagai imunomodulator pada pasien rinitis alergika. Penelitian ini dilaksanakan dari bulan Juni-Desember 2011 di laboratorium Fisiologi Fakultas Kedokteran Unsyiah Banda Aceh dan pemeriksaan IgE dilakukan di laboratorium swasta. Desain penelitian adalah quasi experimental dengan rancangan pretest-posttest with control group. Subjek penelitian adalah 12 pasien rinitis alergika, yaitu 6 orang sebagai kelompok kontrol (diberikan plasebo) dan 6 orang sebagai kelompok perlakuan (diberikan kapsul polifenol teh hijau 2x350 mg/hari, selama 14 hari). Analisis data menggunakan uji normalitas, uji homogenitas, dan uji-t (p<0,05). Hasil penelitian menunjukkan kadar imunoglobulin E sesudah pemberian perlakuan pada kelompok perlakuan lebih rendah dibanding dengan kelompok kontrol (1.475,2±940,7 vs 494,3±366,5 IU), namun tidak bermakna (p=0,05). Simpulan pemberian polifenol teh hijau menurunkan sekresi IgE, namun tidak bermakna dan membutuhkan penelitian lebih lanjut. [MKB. 2015;47(3):160-66] Kata kunci: Imunoglobulin E, imunomodulator, polifenol teh hijau (Camellia sinensis L.), rinitis alergika Activity of Green Tea (Camellia sinensis (L) O. Kuntze) Polyphenols as Immunomodulator through Response of Suppression Immunoglobulin E (IgE) in Allergic Rhinitis AbstractAllergic rhinitis is an inflammatory condition of the nasal mucosa caused by interactions between allergens and immunoglobulin E (IgE). Immunomodulatory is an important part of the treatment of allergic rhinitis. One of the medicinal plants that have immunomodulatory activities is green tea (Camellia sinensis L.), specifically polyphenols. The purpose of this study was to analyze the activity of green tea's polyphenols as an immunomodulator in patients with allergic rhinitis. This study was conducted in June to December 2011 in the laboratory of Physiology, Faculty of Medicine Unsyiah with the IgE examinations conducted in private laboratories. This study is a pretest-postest quasi experimental study with control group design. Subjects were 12 patients with allergic rhinitis; 6 people were included in the control group (placebo) and 6 in the treatment group (green tea's polyphenols 2x350 mg/day, for 14 days). Analysis of the data was performed using the normality test, homogeneity test, and t-test (p<0.05).The results showed that the levels of immunoglobulin E after the administration of green tea's polyphenols in the treatment group was lower than the control group (1.475.2±940.7 vs 494.3±366.5 IU), but not significantly (p=0.05). In conclusion, the administration of green tea's polyphenols can insignificantly decrease the secretion of IgE. Hence, further resea...

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“…There are some reports on IgE suppression by food components such as flavones (Yano et al 2007;Hassanain et al 2010). We also found that some species of strawberries (Fragaria x ananassa) suppressed total IgE production in in vitro immunization (IVI) where a population of human peripheral blood lymphocytes was cultured with the ceder pollen antigen, Cry j 1, in a medium containing human plasma, fetal bovine serum (FBS), IL-2, IL-4, IL-6, and muramyl dipeptide (Mitsuda et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Purification and identification of an IgE suppressor from strawberry in an in vitro immunization system

et al. 2012

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We purified and identified an IgE suppressor from the strawberry 'Toyonoka', based on the decrease of IgE production in in vitro immunization (IVI). Gel filtration experiment indicated that fractions in a 15-48 kDa range and <10 kDa have an IgE suppressive activity. Furthermore, the fraction in 15-48 kDa was subjected to chromatofocusing and found to have activities at isoelectric points, pI 6.0, 7.0, and 8.0-9.2. We focused on the active fractions of pI 8.0-9.2 and the purified a large amount of strawberry extracts by cation exchange resins in batch. A purified 39 kDa protein showed homology to plant glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in N-terminal amino acid sequence and had GAPDH enzymatic activity. Nucleotide sequence and deduced amino acid sequence of the obtained cDNA clone of the protein matched with the sequence of Fragaria x ananassa GAPDH in the GenBank with >98% identical nucleotides and >99% identical amino acids, respectively. The purified strawberry GAPDH suppressed total IgE production in IVI in a dose-dependent manner. From these results, we identified GAPDH as IgE suppressor in the strawberry. Our study may be applicable to the development of new methods to relieve allergic conditions using GAPDH and the screening of other functional factors for human health.

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Green Tea (Camelia Sinensis) Suppresses B Cell Production Of IgE Without Inducing Apoptosis

Cited by 17 publications

References 0 publications

Identification of Dimethylmatairesinol as an Immunoglobulin E-suppressing Component of the Leaves of Cinnamomum camphora

Identification of Dimethylmatairesinol as an Immunoglobulin E-suppressing Component of the Leaves of Cinnamomum camphora

Aktivitas Polifenol Teh Hijau (Camellia sinensis (L) O. Kuntze) Sebagai Imunomodulator melalui Respons Supresi Imunoglobulin E (IgE) pada Rinitis Alergika

Purification and identification of an IgE suppressor from strawberry in an in vitro immunization system

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