Green Synthesized Silver Nanoparticles Using Tridax Procumbens for Topical Application: Excision Wound Model and Histopathological Studies

Abstract

The objective of this study was to synthesize silver nanoparticles from the leaves of Tridax procumbens and develop its topical gels using chitosan to investigate the wound healing efficacy concomitant with the histopathological study. Green synthesized silver nanoparticles (AgNPs) were prepared by reacting silver nitrate (0.3 M) with leaf extract and characterized by particle analysis, FTIR, XRD, SEM, BET, and TGA. The results revealed formed AgNPs were nano-sized (138 ± 2.1 nm), monodispersed (PDI: 0.460 ± 0.3), inter-particle repulsion (zeta: −20.4 ± 5.20 mV), stabilized, crystalline and, spherical with size ranging from 80–100 nm as per SEM micro photos. The BET analysis of AgNPs presents the surface area (12.861 m2/g), pore volume (0.037 cc/g), and pore radius (24.50 nm).TGA results show a loss of 13.39% up to 300 °C. The topical formulation was developed by loading AgNPs in chitosan-based gels, evaluated by pH, thermal cycling, centrifugal, and spreadability tests. AgNPs chitosan gels results showed skin compatibility, higher stability, and spreading ability. The maximum antibacterial zone of inhibition was found to be 25 ± 0.98 mm for bacillus subtitles and 30 ± 1.99 mm for Klebsiella pneumoniae, respectively. Nanosilver-containing gel also showed excellent compatibility with erythrocytes. Excision wound model was used to assess the wound healing property of the developed AgNP gels, the results of which indicated a significantly progressive healing process in test-group of animals treated with chitosan-based gels containing AgNPs. A histopathological study further confirmed the almost normal skin structure of treated animal tissue compared to standard and negative control. Thus, green synthesized AgNPs loaded chitosan-based topical gel can potentially be used for wound healing application.

Green Synthesis of Silver Nanoparticles Coated by Water Soluble Chitosan and Its Potency as Non-Alcoholic Hand Sanitizer Formulation

“…Therefore, chitosan has a synergistic effect, enhancing the antibacterial activity of silver nanoparticles in the formulation of gel hand sanitizers. Silver nanoparticles coated with a polymer (chitosan) have also been successfully synthesized in several previous studies [52,66,67]. A similar diameter of the inhibition zone was found in the present study compared to previous research.…”

“…Silver nanoparticles coated with a polymer (chitosan) have also been successfully synthesized in several previous studies [52,66,67]. A similar diameter of the inhibition zone was found in the present study compared to previous research.…”

Bacterial cellulose as a potential biopolymer in biomedical applications: a state-of-the-art review

“…They are all heavily influenced by available surface area, pore size distribution, and other factors. 314,315 The immunological response to the first stage of wound healing necessitates significant participation of elements from the blood (e.g., granulocytes) and WBC fractions (e.g., macrophages, mast cells, lymphocytes, and neutrophils). To avoid microbial infections, the attracted cells generate many helpful chemicals such as epidermal growth factors, chemokinetic factors, and other substances.…”

Alginate as a Promising Biopolymer in Drug Delivery and Wound Healing: A Review of the State-of-the-Art

“…Skin injuries can be acute or chronic and can occur in arterial insufficiency, diabetes mellitus, immunological disorders, and other infections. Rarely does complete re-epithelialization happen in repairing skin defects; hence, increased attention is diverted to preparing wound dressings to promote wound healing and reduce scars while also protecting from microbial infections and dehydration at the wound site [ 336 , 337 ].…”