Green synthesized multiple fluorescent nitrogen-doped carbon quantum dots as an efficient label-free optical nanoprobe for in vivo live-cell imaging

Atchudan, Raji; Edison, Thomas Nesakumar Jebakumar Immanuel; Perumal, Suguna; Selvam, N. Clament Sagaya; Lee, Yong Rok

doi:10.1016/j.jphotochem.2018.12.011

Cited by 128 publications

(52 citation statements)

References 50 publications

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“…Carbonization or pyrolysis methods were exploited to fabricate multicolor CDs (blue, green, yellow, and red). CDs have found applications in a various areas including biological sensing [17], bioimaging [18, 19, 20, 21], drug delivery, and photovoltaic devices, although several issues such as time-consuming and tedious synthesis and need for expensive instruments in the production processes remain [22, 23, 24, 25]. Thus, low-cost, rapid, and reliable synthesis of high quality and low cytotoxic CDs in large scales is still highly desirable [26].…”

Section: Introductionmentioning

confidence: 99%

Effect of carbonization degree of carbon dots on cytotoxicity and photo-induced toxicity to cells

Esfandiari

Bagheri

Ehtesabi

et al. 2019

Heliyon

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BackgroundPristine carbon dots (CDs) derived from citric acid pyrolysis are used in a variety of biomedical research such as imaging and drug delivery. However, potential cytotoxic effects of pyrolysis temperature on cells is underexplored. To address this need, we studied toxicity of the CDs to breast cancer cells using MTT and LDH assays. In addition, we investigated photo-induced cytotoxicity of the synthesized CDs in a wide concentration range under white light.ResultsOur results suggest little cytotoxicity of the CDs after 24 h exposure of cells. Only the high quantum yield CDs caused a significant toxicity to cells at the highest concentrations of 2.0 and 1.5 mg/ml compared to other CDs at similar concentrations. The synthesized CDs entered the cells without any significant cytotoxicity. The CDs also caused a concentration- and irradiation time-dependent photo-induced cytotoxicity.ConclusionThe optimization of synthesis conditions from this study may help develop safe and efficient CDs for imaging and drug delivery.

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Section: Introductionmentioning

confidence: 99%

Effect of carbonization degree of carbon dots on cytotoxicity and photo-induced toxicity to cells

Esfandiari

Bagheri

Ehtesabi

et al. 2019

Heliyon

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“…Figure 4 shows TEM image of CNs, N-CNs and S-CNs multiple conglomerates of the carbon spheres are abundant and, in some cases, chains of beads-like accretions of the carbon spheres. No obvious spherical assemblies are found in the TEM image of the S-CNs, which is indicative of their amorphous nature [23]. Acid red 57 (AR) and basic fuchsin (BF) adsorption [40] International Journal of Chemical Engineering 3 e specific surface area is estimated by using the Brunauer-Emmett-Teller (BET) manner, and the pore size distributions are found by means of the Barrett-Joyner-Halenda (BJH) method equation using the adsorption isotherm model, which are shown in Figure 5.…”

Section: Tem Images Of Nanospheresmentioning

confidence: 94%

“…e produced solid substance was saved for the other purpose. Pure sulfurand nitrogen-doped carbon nanospheres were obtained via a similar process, but 2 mL of NH 3(aq) solution was added in extract to prepare N-CNs and 1 mL of concentrated sulfuric acid (98%) was added in extract to prepare S-CNs [22,23] Table 1. +2 .…”

Section: Synthesis Of the Carbon Nanospheres Sulfur-andmentioning

confidence: 99%

“…e pattern of CNs shows an intense XRD peak broad in range (2θ = 19-21°), which applies on d-spacing of (4.50, 4.34, and 4.08) A°, while the XRD pattern of S-CNs appear a wide diffraction band at 23.6°that applies on d-spacing of 5.48 A°, where the presence of functional groups on the surface of S-CNs makes it larger interlayer spacing than of CNs. Also, the presence of ammonia groups on the N-CNs surface makes it a more sharp peak than CNs [23,29].…”

Section: Xrd Analysismentioning

confidence: 99%

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Green Synthesis of S- and N-Codoped Carbon Nanospheres and Application as Adsorbent of Pb (II) from Aqueous Solution

Hussain

Alwan

Alshamsi

et al. 2020

International Journal of Chemical Engineering

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In this paper, green and facile synthesis of sulfur-and nitrogen-codoped carbon nanospheres (CNs) was prepared from the extract of Hibiscus sabdariffa L by a direct hydrothermal method. Finally, sulfur-carbon nanospheres (CNs) were used as the adsorbent to remove Pb +2 ions from aqueous solutions because of the high surface area of S-CNs from CNs and N-CNs. e synthesized nanospheres were examined by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy, transmission electron microscopy (TEM), and nitrogen adsorption-desorption isotherms. e results show spherical shapes have a particle size of up to 65 nm with a high surface area capable of absorbing lead ions efficiently. Additionally, the factors affecting the process of adsorption that include equilibrium time, temperature, pH solution, ionic intensity, and adsorbent dose were studied. e equilibrium removal efficiency was studied employing Langmuir, Freundlich, and Temkin isotherm forms. e kinetic data were analyzed with two different kinetic models, and both apply to the adsorption process depending on the values of correlation coefficients. e thermodynamic parameters including Gibbs free energy (ΔG°), standard enthalpy change (ΔH°), and standard entropy change (ΔS°) were calculated for the adsorption process.

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“…Tremendous efforts have been made to develop methods for the preparation of CQDs with controllable particle size, high production yield and adjustable surface functionalization [18,19]. By using the three isomers of phenylenediamine, Jiang et al developed a simple hydrothermal method to synthesize red, green and blue light-emitting CQDs [20].…”

Section: Introductionmentioning

confidence: 99%

Polyethylene Glycol6000/carbon Nanodots as Fluorescent Bioimaging Agents

Chien

et al. 2020

Nanomaterials

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Photoluminescent nanomaterials have immense potential for use in biological systems due to their excellent fluorescent properties and small size. Traditional semiconductor quantum dots are heavy-metal-based and can be highly toxic to living organisms, besides their poor photostability and low biocompatibility. Nano-sized carbon quantum dots and their surface-modified counterparts have shown improved characteristics for imaging purposes. We used 1,3, 6-trinitropyrene (TNP) and polyethylene glycol6000 (PEG6000) in a hydrothermal method to prepare functional polyethylene glycol6000/carbon nanodots (PEG6000/CDs) and analyzed their potential in fluorescent staining of different types of bacteria. Our results demonstrated that PEG6000/CDs stained the cell pole and septa of gram-positive bacteria B. Subtilis and B. thuringiensis but not those of gram-negative bacteria. The optimal concentration of these composite nanodots was approximately 100 ppm and exposure times varied across different bacteria. The PEG6000/CD composite had better photostability and higher resistance to photobleaching than the commercially available FM4-64. They could emit two wavelengths (red and green) when exposed to two different wavelengths. Therefore, they may be applicable as bioimaging molecules. They can also be used for differentiating different types of bacteria owing to their ability to differentially stain gram-positive and gram-negative bacteria.

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Green synthesized multiple fluorescent nitrogen-doped carbon quantum dots as an efficient label-free optical nanoprobe for in vivo live-cell imaging

Cited by 128 publications

References 50 publications

Effect of carbonization degree of carbon dots on cytotoxicity and photo-induced toxicity to cells

Effect of carbonization degree of carbon dots on cytotoxicity and photo-induced toxicity to cells

Green Synthesis of S- and N-Codoped Carbon Nanospheres and Application as Adsorbent of Pb (II) from Aqueous Solution

Polyethylene Glycol6000/carbon Nanodots as Fluorescent Bioimaging Agents

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