Green synthesized monodispersed silver nanoparticles’ characterization and their efficacy against cancer cells

Abstract

Background: In biomedical research, silver nanoparticles have attracted the attention of many scientists in drug delivery and other applications. This study investigates the characterization of biologically synthesized nanoparticles and their efficacy on cancer cells. Methods: The characterization of biologically produced silver nanoparticles was accomplished using UV¬ visible absorption spectroscopy, which showed the maximum absorption at 422 nm. The structural morphology was carried out by transmission electron microscope (TEM) and atomic force microscope (AFM), and the anticancer efficacy of the biosynthesized compound was investigated on two cancer cell lines, namely HT-29 (colon cancer) and EAC. Results: The morphological and structural examination revealed spherical, three-dimensional nanoparticles ranging between 5 to 50 nm. The results of the anticancer experiment, which was conducted using an MTT assay, revealed that the prepared silver had excellent anti-cancer activity against both tested cells, that is EAC cancer and HT-29 (colon cancer) cell lines. Conclusion: This study confirms that silver nanoparticles can be biologically synthesized using Rhizopus stolonifer and used as anticancer substances in cancer therapy and drug delivery.

Polianthes tuberosa-Mediated Silver Nanoparticles from Flower Extract and Assessment of Their Antibacterial and Anticancer Potential: An In Vitro Approach

“…In response to the PTAgNP treatment in the A431 cell line, the control cells emitted a low fluorescence signal while the PTAgNP treated cells exhibited a higher green fluorescent intensity, which indicates the overproduction of ROS. PTAgNPs trigger ROS, followed by oxidative stress; subsequently, the cells will respond by activating pro-inflammatory signaling cascades and inducing cell death by apoptosis [ 48 ].…”

Synthesis, Characterization and Biomedical Application of Silver Nanoparticles

“…The disadvantages of TEM include the need for a large sample section and a high vacuum. Banu et al [240] reported to use TEM (Japanese JEM-3000 F, Jeol) to study the structural characterization of the AgNPs using drop-coated film and it showed spherical shape and 5 to 50 nm in size. Similarly, AgNPs was characterized using TEM and it revealed spherical shape with the size ranging from 13 to 40 nm [241].…”

Characterization and Fabrication of Xerogel Polymeric Nanocomposites and Multifunctional Applications

“…Nevertheless, their de ciencies prevail in requisition for gauze or adjuvants for use in a bleeding wound. Moreover, the ultra-moist environment propelled by HDG is not encouraging in wound mending promotion, as well as the scabbing effect, especially at earlier phase of wound formation [47].…”