In modern nano science and technology the interaction between inorganic nanoparticles and biological structures are the most exciting areas of research. The desire to synthesize nanoparticles, using efficient and green chemistry approaches has led to the use of microorganisms. Hence the present study reports on the screening of potent Lactobacillus spp for ecofriendly and economical synthesis of silver nanoparticles. Total of nine Lactobacillus strains were isolated from milk and milk products inoculated to sterilized milk and latter (after 24hr) the whey was collected by coarse filtration (Whatman no.40). Silver nitrate (1 mg) was added to 5 ml of pale yellow filtrate then incubated at 37⁰C. Absorption maximum was measured using spectrophotometer. The silver nanoparticles of size ranging from 2 to 20nm were synthesized by Lactobacillus sp VRS-2 which was confirmed by TEM and EDS analysis. Thus, rapid and ecofriendly biosynthesis of silver nanoparticles using whey appears to be economical approach and any research in this direction is encouraging.
Biological synthesis of silver nanoparticles by using fungi is reported here. The present study is on screening of filamentous fungi for the production of silver nanoparticles extracellularly.Eight fungi, Rhizopus.spp, Aspergillus terreus, A.flavus, A.niger, A.clavatus, Acremonium.spp, A.rutilum, Trichoderma.sp. have been screened for the production of silver nanoparticle. The fungal filtrates of the above said isolates were subjected to silver nitrate. After incubation, visual observation of brown color is an indication of silver nanoparticle production. Of the eight fungi, only one Rhizopus stolonifer showed maximum absorbance at 422nm. Parametric optimization study showed maximum absorbance at 40 o C and pH 7.0. Further characterization was made by UV-Visible absorption spectroscopy which shows maximum absorption at 422 nm, Transmission Electron Microscope (TEM) revealed the formation of spherical nanoparticles with size ranging between 5 to 50 nm. EnergyDispersive Spectroscope (EDS) shows the optical absorption peak at 3kev, Fourier Transform Infrared (FT-IR) shows the bands at 1645(1), 1537(2) and 1454(3) cm -1 .
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