Green Synthesize and Characterization of Copper Nanoparticles Using Iranian Propolis Extracts

Abstract

The propolis produced by bees is used by them to protect their hives. The cavity inside the hive’s walls is filled in during cold days to reduce entry points and mummify any intruders to ensure their survival. A current focus in nanotechnology and nanoscience is the green biosynthesis of nanoparticles (NPs) using biomaterials. Research on green methods for making metal oxide NPs is gaining momentum to safeguard the environment from the potential dangers associated with toxic chemicals. This study aimed to synthesize copper NPs (CuNPs) via propolis extraction, a novel application of nanoscience. The study was conducted under a range of pH, time conditions, and concentration ratios, and its properties were characterized by UV-Vis absorption spectra, XRD, and FTIR. An FTIR analysis revealed that compounds found in propolis extract could have an effect on the surface modification of the synthesized NPs. The propolis (Khalkhal) extract spectrum exhibited a sharp peak at 3422 cm−1, caused by free hydroxyl groups and their intra/intermolecular hydrogen bonds. There were sharp peaks at 2925, 1637, and 1515 to 1076 cm−1 associated with the C = O and C = C aromatic stretching frequencies. According to UV-Vis spectrophotometry investigation, CuO NPs exhibit a characteristic peak at 385 nm, showing significant surface plasmon resonance (SPR) with propolis (Khalkhal) extract. Furthermore, specific wavelengths of CuO NPs demonstrate peaks at 243, 292, and 350 nm for propolis (Gilan) extract. The green synthesis of CuNPs from Gilan and Khalkhal propolis can be an appropriate candidate for clinical applications such as drug delivery systems, drug formulation, and biomedical applications.

Evaluation of antibacterial and antifungal properties of copper oxide nanoparticles after combination with tissue conditioner used in complete prosthesis

“…The green biosynthesis of CuO nanoparticles was successfully conducted using a non-toxic, cost-effective, easy, and eco-friendly approach. These copper nanoparticles will probably be used in pharmaceutical formulations, drug delivery systems, and biomedical applications in the future since they can be prepared from natural products using a green biosynthesis method [32]. According to the ndings from XRD, FTIR, and TEM investigations, the CuO nanoparticles made in the present study had monoclinic crystalline structures.…”

The Effect of Cytotoxicity and Antimicrobial of Synthesized CuO NPs from Propolis on HEK‐293 Cells and Lactobacillus acidophilus

“…CSp3-H and aromatic stretching frequencies of C�O and C�C were related to the peaks at 2920, 1614, and 1515 to 1057 cm −1 . An absorption band of 603 cm −1 was observed in the monoclinic phase of CuO NPs [35].…”

“…According to FTIR analysis, propolis extract compounds were found to modify the surface of synthesized NPs. Tere was a sharp peak at 3422 cm −1 [35].…”

Nano Propolis, Zinc Oxide Nanoparticles, and Their Composites: A Novel Green Synthesis with Synergistic Antioxidant and Anticancer Properties

“…Owing to propolis containing reducing agents and stabilizing biocompounds, it has a potential application in the green synthesis of metal NPs as selenium, iron, copper, and silver nanoparticles [40][41][42][43][44][45]. However, there is a lack of studies on producing ZnO NPs using propolis.…”

“…silver nanoparticles [40][41][42][43][44][45]. However, there is a lack of studies on producing ZnO NPs using propolis.…”