Background
Tissue conditioners are used for treating and improving the tissues supporting complete dentures. On the other hand, recent advances in nanotechnology have revolutionized various fields of science, including dentistry. Moreover, some nanoparticles exert antimicrobial properties.The present study aimed to investigate the antimicrobial properties of copper oxide nanoparticle-based tissue conditioner used in complete prostheses.
Methods
The present experimental study included 126 tissue conditioner samples with different concentrations of copper oxide nanoparticles (0%, 0.625%, 1.25%, 2.5%, 5%, 10%, 20% w/w). The samples were incubated with Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans in 24-well plates for 24 hours. Then, samples from the wells were re-incubated for 24 hours, and the microorganisms were counted.
Results
The culture media containing E. faecalis and P. aeruginosa showed significantly different growth between different nanoparticle concentrations following 24 hours (P < 0.001), showing a reduction in bacterial growth with increased nanoparticle concentration. Both bacteria did not show any growth at the 20% concentration. However, C. albicans showed significant differences in growth between different nanoparticle concentrations following 48 hours (P < 0.001), showing a reduction in growth with increased nanoparticle concentration. Also, the least growth was observed at the 20% concentration.
Conclusions
In the present study, the CuO nanoparticles were made properly in the suitable sizes. Moreover, the tissue conditioners containing copper oxide nanoparticles showed acceptable antimicrobial properties against E. faecalis, P. aeruginosa, and C. albicans.