Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties

Abstract

Background The growing threat of microbial resistance against traditional antibiotics has prompted the development of several antimicrobial nanoparticles (NPs), including silver NPs (AgNPs). In this article, a simple and eco-friendly method for the synthesis of AgNPs using the cranberry powder aqueous extract is reported. Materials and methods Cranberry powder aqueous extracts (0.2%, 0.5%, and 0.8% w/v) were allowed to interact for 24 hours with a silver nitrate solution (10 mM) at 30°C at a ratio of 1:10. The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy and their concentrations were determined using atomic absorption spectroscopy. The prepared NPs were evaluated by transmission electron microscopy, measurement of ζ-potential, and Fourier-transform infrared spectroscopy. The in vitro antimicrobial properties of AgNPs were then investigated against several microbial strains. Finally, in vivo appraisal of both wound-healing and antimicrobial properties of either plain AgNPs (prepared using 0.2% extract) or AgNP-Pluronic F-127 gel was conducted in a rat model after induction of a Staphylococcus aureus ATCC 6538P wound infection. Results The formation of AgNPs was confirmed by ultraviolet-visible spectroscopy, where a surface-plasmon resonance absorption peak was observed between 432 and 438 nm. Both size and concentration of the formed AgNPs increased with increasing concentration of the extracts. The developed NPs were stable, almost spherical, and polydisperse, with a size range of 1.4–8.6 nm. The negative ζ-potential values, as well as Fourier-transform infrared spectroscopy analysis, indicated the presence of a capping agent adsorbed onto the surface of the particles. In vitro antimicrobial evaluation revealed a size-dependent activity of the AgNPs against the tested organisms. Finally, AgNPs prepared using 0.2% extract exhibited a substantial in vivo healing potential for full-thickness excision wounds in rats. Conclusion AgNPs were successfully synthesized from a silver nitrate solution through a simple green route, using cranberry powder aqueous extract as a reducing as well as capping agent.

“Smart” Antimicrobial Nanocomplexes with Potential to Decrease Surgical Site Infections (SSI)

“…The ζ-potential values of AgNP dispersions (Table 1) proved the efficiency of the capping agents in stabilizing the NPs by providing intensive negative charges. This result is in agreement with previous investigations using cranberry powder aqueous extract [89]. In our compounds the ζ-potential values change with the introduction of PI to higher negative zeta potentials (Table 1).…”

Silver nanoparticles-based plasmonic assay for the determination of sugar content in food matrices

“…This red-shift is caused by an increase of the adsorbed layer around the MNPs [13,16,17] due to the increasing sugars shell around the AgNPs core [16]. This behavior has been already reported for AgNPs formed with increasing concentration of natural compounds [46][47][48]. In our conditions, the red shift was ≤ 18 nm (from the lower to the higher concentration) for all the tested sugars.…”

Phytosynthesis of Silver Nanoparticles Using Perilla frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities

“…39 After confirmation of phytosynthesis, the solution of PF@AgNPs was centrifuged for 20 min at 15,000 × g. The supernatant was removed and the pellet was redispersed in distilled water and centrifuged again for 20 min at 15,000 × g. This procedure was repeated for three times. 40 Finally, the pellet was collected and dried into a fine powder that was used for further analysis. Fourier transform infrared (FTIR) analysis of PF@AgNPs was carried out at 4000-500 cm −1 using KBr pellets (Perkin-Elmer Ltd).…”

“…47,48 Ascorbic acid was used as the standard antioxidant. In DPPH assay, PF@AgNPs (20,40,60,80, and 100 µg) were dissolved in 1 mL methanol in separate test tubes. Then, 2 mL DPPH stock solution (1 mM/L) was added, and the tubes were incubated in the dark for 45 min.…”

“…In ABTS assay, ABTS radical working solution was prepared prior to the assay. ABTS radical working solution (0.9 mL) was added to 0.1 mL of the different concentrations (20,40,60,80, and 100 µg/mL) of PF@AgNPs, shaken well for 45 sec, and incubated in the dark for 15 min, followed by measurement of the absorbance at 734 nm. DPPH/ ABTS scavenging activity was calculated using the equation: % scavenging = [(control absorbance − test compound absorbance)/control absorbance] × 100.…”