Green Synthesis of Silver Nanoparticles from Phaseolus vulgaris L. Extracts and Investigation of their Antifungal Activities

Abstract

B u çalışmanın amacı, Phaseolus vulgaris L.'nin (Yunus-90) yaprak, kök ve gövde özütlerini kullanarak gümüş nanopartiküllerini (AgNPs) sentezlemek ve antifungal aktivitelerini ölçmektir. Bu bağlamda, hazırlanan AgNP'ler UVvis, FT-IR, TEM, SEM ve DLS teknikleri kullanılarak karakterize edilmiştir. Ardından hem sentezlenen hem de ticari olarak satın alınan AgNP'lerin Colletotrichum sp., Fusarium oxysporum, Fusarium acuminatum, Fusarium tricinctum, Fusarium graminearum, Fusarium incarnatum, Rhizoctonia solani, Sclerotinia sclerotiorum ve Alternaria alternata'ya karşı antifungal aktivitesi; (i) agar kuyu difüzyonu, (ii) mantar kolonisinin morfolojik çeşitliliği, (iii) hif inhibisyonu ve (iv) minimum inhibisyon konsantrasyonu (MIC) analizleri ile araştırılmıştır. Yaprak özütlerinden türetilen AgNP'ler, kök ve gövde özütlerinden hazırlanan AgNP'lere göre önemli ölçüde yüksek antifungal aktivite sergilemiştir. Ticari AgNP'ler ayrıca bu araştırmada sentezlenen yeşil eşdeğerlerinden daha düşük antifungal aktivite göstermiştir. Ayrıca, sentezlenen AgNP'lerin düşük (~ 50 μg/ mL) konsantrasyonunun bile Fusarium tricinctum ve Colletotrichum sp'nin gelişimini baskılamada etkili olduğu bulunmuştur.

Green Synthesis of Silver Nanoparticles Using Walnut Shell Powder and Cynara sp. and their Antibacterial Activities

“…Silver nanoparticles are reported to have possible diverse pharmacological applications like applications demanding antimicrobial effect on bacterial strains [34,35], cancer therapy [3,25,31,36] and antifungal practices [37,38]. The preparation of metallic nano particles for cancer therapy via green synthesis makes them more reliable in terms of minimizing the possible side effects and less threatening the patient's health [3].…”

Beauveria bassiana biogenic nanoparticles for the control of Noctuidae pests

“…13,14 Metallic nanoparticles obtained through biogenic synthesis have shown promising results. Several studies report the application of these new nanomaterials for the control of diseases such as those caused by phytopathogenic fungi [15][16][17][18] and insects. [19][20][21] Capping by organic compounds from the reducing and stabilizing agents used in their synthesis is a relevant characteristic of biogenic metallic and oxide metallic nanoparticles.…”

“…Metallic nanoparticles obtained through biogenic synthesis have shown promising results. Several studies report the application of these new nanomaterials for the control of diseases such as those caused by phytopathogenic fungi 15–18 and insects 19–21 …”