Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines

Khorrami, Sadegh; Zarepour, Atefeh; Zarrabi, Ali

doi:10.1016/j.btre.2019.e00393

Cited by 69 publications

(44 citation statements)

References 45 publications

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“…Due to the fact that black pomegranate peel extract is rich in phenolic compounds, the loading step was confirmed by the peaks attributing to the phenolic structures. This implies that the synthesized CCMNPs have properly been loaded with black pomegranate peel extract (Khorrami et al 2019). The recorded zeta potential of the MNPs and CCMNPs manifested that the synthesized nanoparticles form a stable ferrofluid (Martinkova et al 2018).…”

Section: Discussionmentioning

confidence: 95%

“…The mixture was then sonicated for 8 min and purified using Whatman qualitative filter paper Grade. 1 (Merck, Germany) (Zhang et al 2018;Khorrami et al 2019).…”

Section: Preparation Of Bppementioning

confidence: 99%

“…The cells were then moved to 96 well plates with 2 × 10 5 cells in each well and incubated. Following this step, the cell media were removed and replaced with media containing 1000 to 7.8 µg/ml of BPPE, BPPE-loaded-CCMNPs, and MNPs, which were prepared by serial dilution (Khorrami et al 2019;Fatahi et al 2020). After each period of incubation (24 h and 48 h), the cell media were removed and replaced with PBS dissolved MTT (5 mg/ml) and incubated for 4 h. The MTT salt was afterward washed out with DMSO and the optical density of the samples was recorded by ELISA plate reader (ELX 808 IU Biotek) at λ max = 570 nm.…”

Section: In Vitro Cytotoxicity Assaymentioning

confidence: 99%

“…Since flavonoids such as anthocyanins could be found in red, blue, and purple plant pigments (Mazza and Miniati 2018), it could be determined that black peel pomegranate has a higher amount of such compounds. Therefore, it could be more efficient for therapeutic purposes (Khorrami et al 2019).…”

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confidence: 99%

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Breast cancer drug delivery by novel drug-loaded chitosan-coated magnetic nanoparticles

2021

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Background Breast cancer is one of the most challenging cancers among women which is considered one of the most lethal cancers to this date. From the time that cancer has been discovered, finding the best therapeutic method is still an ongoing process. As a novel therapeutic method, nanomedicine has brought a vast number of materials that could versatilely be used as a drug carrier. The purpose of this study is to develop a novel black pomegranate peel extract loaded with chitosan-coated magnetic nanoparticles to treat breast cancer cells. Results The morphology and size distribution of the nanoparticles studied by dynamic light scattering, atomic force microscopy, scanning, and transitional electron microscopy showed the spherical shape of the nanoparticles and their promising size range. Studies by Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometer, and zeta sizer confirmed the synthesis, substantial crystallinity, magnetic potential of the nanoparticles, and their satisfactory stability. The DPPH assay revealed that the obtained black pomegranate peel extract has 60% free radical scavenging activity. The cytotoxicity studies by MTT and LDH assay carried out on NIH/3T3, MBA-MB-231, and 4T1 cells confirmed that the magnetic nanoparticles had no significant cytotoxicity on the cells. However, the drug-loaded nanoparticles could significantly eradicate cancerous cells which had more efficiency comparing to free drug. Furthermore, free drug and drug-loaded nanoparticles had no toxic effect on normal cells. Conclusion Owing to the results achieved from this study, the novel drug-loaded nanoparticles are compatible to be used for breast cancer treatment and could potentially be used for further in vivo studies.

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Section: Discussionmentioning

confidence: 95%

“…The mixture was then sonicated for 8 min and purified using Whatman qualitative filter paper Grade. 1 (Merck, Germany) (Zhang et al 2018;Khorrami et al 2019).…”

Section: Preparation Of Bppementioning

confidence: 99%

Section: In Vitro Cytotoxicity Assaymentioning

confidence: 99%

mentioning

confidence: 99%

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Breast cancer drug delivery by novel drug-loaded chitosan-coated magnetic nanoparticles

2021

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“…However, AgNPs showed more cytotoxicity as compared to extract alone. Synthesized nanoparticles significantly enhanced and led to more than 81% and 89% cell death against BT‐20 and MCF‐7; however, extract induced more than 70% cell death against both the cancerous cell lines (Khorrami et al, 2019).…”

Section: Biomedical Applicationsmentioning

confidence: 99%

A review on recent developments in the biosynthesis of silver nanoparticles and its biomedical applications

Arif

Uddin

2020

Med Devices & Sens

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Green chemistry is the significant field of research as it offers simple, cost-effective, easy, biocompatible and eco-friendly methods for the synthesis of nanomaterials. With the development of nanotechnology, the synthesis of silver nanoparticles (AgNPs) has become important areas of research due to their various applications in industrial, pharmacological and medicinal fields as they exhibited good antibacterial, antifungal, antioxidant, anti-inflammatory, antiviral, anticoagulant, thrombolytic, cytotoxic and photocatalytic properties due to their chemical stability and good biocompatibility.How to cite this article: Arif R, Uddin R. A review on recent developments in the biosynthesis of silver nanoparticles and its biomedical applications.

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A comprehensive view on the fisetin impact on colorectal cancer in animal models: Focusing on cellular and molecular mechanisms

Zamanian,

Taheri,

Ramadan

et al. 2024

Anim Models and Exp Med

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Flavonoids, including fisetin, have been linked to a reduced risk of colorectal cancer (CRC) and have potential therapeutic applications for the condition. Fisetin, a natural flavonoid found in various fruits and vegetables, has shown promise in managing CRC due to its diverse biological activities. It has been found to influence key cell signaling pathways related to inflammation, angiogenesis, apoptosis, and transcription factors. The results of this study demonstrate that fisetin induces colon cancer cell apoptosis through multiple mechanisms. It impacts the p53 pathway, leading to increased levels of p53 and decreased levels of murine double minute 2, contributing to apoptosis induction. Fisetin also triggers the release of important components in the apoptotic process, such as second mitochondria‐derived activator of caspase/direct inhibitor of apoptosis‐binding protein with low pI and cytochrome c. Furthermore, fisetin inhibits the cyclooxygenase‐2 and wingless‐related integration site (Wnt)/epidermal growth factor receptor/nuclear factor kappa B signaling pathways, reducing Wnt target gene expression and hindering colony formation. It achieves this by regulating the activities of cyclin‐dependent kinase 2 and cyclin‐dependent kinase 4, reducing retinoblastoma protein phosphorylation, decreasing cyclin E levels, and increasing p21 levels, ultimately influencing E2 promoter binding factor 1 and cell division cycle 2 (CDC2) protein levels. Additionally, fisetin exhibits various effects on CRC cells, including inhibiting the phosphorylation of Y‐box binding protein 1 and ribosomal S6 kinase, promoting the phosphorylation of extracellular signal‐regulated kinase 1/2, and disrupting the repair process of DNA double‐strand breaks. Moreover, fisetin serves as an adjunct therapy for the prevention and treatment of phosphatidylinositol‐4,5‐bisphosphate 3‐kinase catalytic subunit α (PIK3CA)‐mutant CRC, resulting in a reduction in phosphatidylinositol‐3 kinase (PI3K) expression, Ak strain transforming phosphorylation, mTOR activity, and downstream target proteins in CRC cells with a PIK3CA mutation. These findings highlight the multifaceted potential of fisetin in managing CRC and position it as a promising candidate for future therapy development.

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Green synthesis of silver nanoparticles at low temperature in a fast pace with unique DPPH radical scavenging and selective cytotoxicity against MCF-7 and BT-20 tumor cell lines

Cited by 69 publications

References 45 publications

Breast cancer drug delivery by novel drug-loaded chitosan-coated magnetic nanoparticles

Breast cancer drug delivery by novel drug-loaded chitosan-coated magnetic nanoparticles

A review on recent developments in the biosynthesis of silver nanoparticles and its biomedical applications

A comprehensive view on the fisetin impact on colorectal cancer in animal models: Focusing on cellular and molecular mechanisms

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