Green synthesis of silver nanoparticles by Rivina humilis leaf extract to tackle growth of Brucella species and other perilous pathogens

Raghava, Sri; Mbae, K. M.; Umesha, S.

doi:10.1016/j.sjbs.2020.10.034

Cited by 15 publications

(12 citation statements)

References 39 publications

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“…Furthermore, the minimum inhibitory concentration (25µg/ml) also shows better activity which are displayed in Table 1. Other than that, silver nanoparticles synthesised with aqueous extract of Rivina humilis leaves and Pistacia atlantica leaf extract were also strongly inhibited at low concentrations in the growth of all tested bacteria [55,56]. Also our results strongly committed with old reports, that the AgNPs exhibited more antibacterial activity than other nanoparticles because of their physical and chemical properties [57,58].…”

Section: Abts Radical Scavenging Activitysupporting

confidence: 86%

Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application

sekar

2021

Preprint

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A simple and eco-friendly method for the green synthesis of silver nanoparticles (AgNPs) by ultrasound-assisted strategy using Barleria buxifolia leaf extract as a reducing and capping agent was established in this study. The obtained AgNPs were characterized. UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning and transmission electron microscopy (SEM and TEM), Energy Dispersive X-Ray Analyzer (EDX), X-ray diffraction, dynamic light scattering (DLS) analysis showed that the obtained AgNPs were mono dispersed spheres with uniform size of 80 nm. UV-vis spectroscopy, FTIR, and XRD analysis indicated that the surface of the obtained AgNPs was covered with organic molecules in plant extracts. The results of ABTS assays showed that high antioxidant activity was seen in the obtained AgNPs. Green synthesized AgNPs showed potent antibacterial and anti-biofilm activity against tested pathogens. Cytotoxicity assay showed that the obtained AgNPs were significantly cytotoxic to cancer cell line (MCF-7). In addition, the AgNPs synthesized in this paper can also photo catalytically degrade methylene blue dye under visible light. The potent bioactivity exhibited by the green synthesized silver nanoparticles leads towards the multiple use as antioxidant, antibacterial, anti-biofilm, cytotoxic as well as photo catalytic agent.

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Section: Abts Radical Scavenging Activitysupporting

confidence: 86%

Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application

sekar

2021

Preprint

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“…Brucellosis is a resurgent bacterial endemic infectious disease that affects domestic and wild animals as well as humans. Mammalian brucellosis is expected to affect approximately one million people each year, with 40% of cases progressing to chronic illness [ 6 , 7 ]. A mixture of antibiotics is the recommended treatment for human brucellosis induced by clinical strains of Brucella melitensis ( B .…”

Section: Introductionmentioning

confidence: 99%

“…abortus ) [ 8 – 10 ]. Nevertheless, there is a significant probability of the recurrence of illness and therapeutic failure due to increasing resistance; thus, the fundamental concern for brucellosis management is that the pathogenesis of the condition is important [ 7 ]. As a result, a new approach to treat brucellosis is greatly needed.…”

Section: Introductionmentioning

confidence: 99%

Brucella species-induced brucellosis: Antimicrobial effects, potential resistance and toxicity of silver and gold nanosized particles

et al. 2022

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Brucellosis is an endemic zoonotic disease caused by Brucella species, which are intramacrophage pathogens that make treating this disease challenging. The negative effects of the treatment regime have prompted the development of new antimicrobials against brucellosis. A new treatment modality for antibiotic-resistant microorganisms is the use of nanoparticles (NPs). In this study, we examined the antibacterial activities of silver and gold NPs (SNPs and GNPs, respectively), the resistance developed by Brucella melitensis (B. melitensis) and Brucella abortus (B. abortus) strains and the toxicity of both of these NPs in experimental rats. To test the bactericidal effects of the SNPs and GNPs, we used 22 multidrug-resistant Brucella isolates (10 B. melitensis and 12 B. abortus). The minimal inhibitory concentrations (MICs) of both types of NPs were determined utilizing the microdilution technique. To test the stability of resistance, 7 B. melitensis and 6 B. abortus isolates were passaged ten times in culture with subinhibitory concentrations of NPs and another ten times without NPs. Histopathological analysis was completed after rats were given 0.25, 0.5, 1, and 2 mg/kg NPs orally for 28 consecutive days. The MIC values (μg/ml) of the 10-nm SNPs and 20-nm GNPs against B. melitensis were 22.43 ± 2.32 and 13.56 ± 1.22, while these values were 18.77 ± 1.33 and 12.45 ± 1.59 for B. abortus, respectively. After extensive in vitro exposure, most strains showed no resistance to the 10-nm SNPs or 20-nm GNPs. The NPs and antibiotics did not cross-react in any of the evolved Brucella strains. SNPs and GNPs at doses below 2 mg/kg were not harmful to rat tissue according to organ histopathological examinations. However, a greater dose of NPs (2 mg/kg) harmed all of the tissues studied. The bactericidal properties of NPs are demonstrated in this work. Brucella strains develop similar resistance to SNPs and GNPs, and at low dosages, neither SNPs nor GNPs were hazardous to rats.

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“…Rh−AgNP's demonstrated potential anti‐brucellosis activity with effective inhibition at 800 μg/mL. Rh−AgNP's showed strong antimicrobial activity with prospective application in the biological sphere [226] …”

Section: Nanomaterials For the Treatment Of Zoonotic Diseasesmentioning

confidence: 99%

“…RhÀ AgNP's showed strong antimicrobial activity with prospective application in the biological sphere. [226] Mohammadi and co-workers synthesized silver and copper nanoalloys (AgÀ Cu) via microbial reduction of AgNO 3 and CuSO 4 by Lactobacillus casei ATCC39392. These NPs were characterized via in-vitro, ex-vivo, and in-vivo techniques.…”

Section: Rabies and Brucellosismentioning

confidence: 99%

Nanotechnology for Therapy of Zoonotic Diseases: A Comprehensive Overview

et al. 2022

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Zoonotic infections belong to multiple infectious diseases transferred from animals to humans. Now, the treatment and diagnosis of zoonotic infections are perplexing due to genetic mutations, target site modifications, and multi‐drug resistance. Despite their benefits, most diagnostic molecular techniques have certain limits in terms of repeatability and sensitivity, mainly due to the heterogeneity among the diverse family of zoonotic pathogens. Therefore, developing more efficient and cost‐effective theranostics tools is the need of the hour to address these concerns. For this purpose, nanotechnology has revolutionized medicine with versatile potential capabilities for diagnosing and treating zoonosis via the targeted and controlled delivery of antimicrobial drugs via binding to the overexpressed infectious macrophages. Massive advancements have been made in fabricating novel nano‐based formulations to control zoonosis based on the use of poly(ethylenimine)‐conjugated nanomicelles, mannosylated thiolated chitosan (MTC)‐coated PM‐loaded PLGA NPs, mannose linked thiolated nanocarriers, adjuvanted pDNA hydrogel, arginine‐based nanocarriers, quantum dots to treat and diagnose a wide range of zoonotic diseases, including zoonotic influenza, salmonellosis, leishmaniasis, rabies, brucellosis, Lyme Disease, tuberculosis, and other infections caused by West Nile Virus, emerging coronaviruses (SARS, MERS, COVID‐19), in a preferentially targeted way. Recently developed anti‐pathogen loaded‐nanoformulations with enhanced cellular uptake, biocompatibility, and hemocompatibility have shown the ability to cross biological barriers when orally administrated. Therefore, this article reviewed the latest milestones and future growth areas in the field of efficient theranostics platforms to manage zoonotic infections.

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Green synthesis of silver nanoparticles by Rivina humilis leaf extract to tackle growth of Brucella species and other perilous pathogens

Cited by 15 publications

References 39 publications

Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application

Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application

Brucella species-induced brucellosis: Antimicrobial effects, potential resistance and toxicity of silver and gold nanosized particles

Nanotechnology for Therapy of Zoonotic Diseases: A Comprehensive Overview

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